1R8C,2ZH7,1PX5


Conserved Protein Domain Family
NT_2-5OAS_ClassI-CCAase

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cd05400: NT_2-5OAS_ClassI-CCAase 
Click on image for an interactive view with Cn3D
Nucleotidyltransferase (NT) domain of 2'5'-oligoadenylate (2-5A)synthetase (2-5OAS) and class I CCA-adding enzyme.
In vertebrates, 2-5OASs are induced by interferon during the innate immune response to protect against RNA virus infections. In the presence of an RNA activator, 2-5OASs catalyze the oligomerization of ATP into 2-5A. 2-5A activates endoribonuclease L, which leads to degradation of the viral RNA. 2-5OASs are also implicated in cell growth control, differentiation, and apoptosis. This family includes human OAS1, -2, -3, and OASL. CCA-adding enzymes add the sequence [cytidine(C)-cytidine-adenosine (A)], one nucleotide at a time, onto the 3' end of tRNA, in a template-independent reaction. This class I group includes the archaeal Sulfolobus shibatae and Archeoglobus fulgidus CCA-adding enzymes. It belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, coordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are conserved in this family.
Statistics
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PSSM-Id: 143390
View PSSM: cd05400
Aligned: 72 rows
Threshold Bit Score: 59.3357
Threshold Setting Gi: 56476813
Created: 27-Jun-2007
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Based on similarity to Archaeoglobus fulgidus CCA-adding enzyme. Active site includes NTP-, metal-, and RNA-binding residues.
  • Structure:1R8C_A, Archaeoglobus fulgidus CCA-adding enzyme bound with UTP and Mn ions, contacts determined at 4A
    View structure with Cn3D
  • Structure:2ZH7_A; A. fulgidus CCA-adding enzyme bound with a tRNA minihelix, contacts determined at 4A.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                              ###  # ###        # #                     
1R8C_A        16 PDEEEVRKGREAEEELRRRLDel---------gVEYVFVGSYARNTWLkg----sLEIDVFLLFPeefs------keelR 76
gi 86475969   20 ELSELANKRDILLKKLRDSDDip---------sFEEFNQGSYSMFTGVepl-dkeYDIDVGLRFNvnks------dyepL 83
gi 117676292  22 TYREARSKDSSILDDLRDEFKnag------ypiIETFIQGSLSTHTGIkhp-vadFDIDRAVVIDadna------pddpV 88
gi 10957170   20 KKESISLSISTLHSRLKEYFGkd---------iIDVIKFGSYTRGTILprryddkSDVDIMIVFNndyd-------ykpQ 83
gi 83591054   50 KTDEIRQHLETIRGALDKNIEg----------aIELVFGGSLSKHTYVng----lSDIDMLVRINdtsla-----naspA 110
gi 41614948   16 DYEKAKKALDYIIPILEKTPHi-----------YDVFVGGSYAKGTWLg------RDIDIFVRFPkky---------kgQ 69
gi 115421463  20 EQASINASIVTIKSRLNNYFGta---------lSEQLRFGSSTRGTILprkmdqhSDIDYMVVFAqgg--------avpQ 82
gi 156390715 100 LKDHASEALDAIFKKIQEDDKlq---------iRELVKAGSLAKGTALkd----sSDLDCVMIMNgiet------vadlK 160
gi 4468185    14 NLTDANCAVKEIVECIQSLQSysvgeeqvgfsvRDIIKAGSLGHGTSVeg----nYDIDLVVYSTeisaydvlraeshfQ 89
gi 116242687  30 CQTLIDEMVNTICDVLQEPEQfp--------lvQGVAIGGSYGRKTVLrg----nSDGTLVLFFSdlkqfq--dqkrsqR 95
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                   ##   #                 #                             
1R8C_A        77 ERGLEIGKAVldsy---------eiryAEHP-YVHGVVk-------gVEVDVVPCYKLkepk------------------ 121
gi 86475969   84 YLKEKIQEVLknhte--------ygadIKQP-CVTVKYkkdg--eiaYHIDLVVYSYEdkndkesqmyla---------- 142
gi 117676292  89 EVKKLVLKVLekrgf--------knarIKKP-CVTADYmn-----lnLHIDIVIYRKLgqqyelavgk------------ 142
gi 10957170   84 TFLDRLKKFAqqkys-------tsyvyQSNP-TIVLELn-------hIKLELVPAVKIyswsddyy-------------- 134
gi 83591054  111 DIKAYFAERLlkrlp-------nteveVGKL-AVTVRFss-----tgHEIQLLPALQTktgvria--------------- 162
gi 41614948   70 NISIYIEQTLkehnvp------yiklhGSRD-YFQTFYe-------gLKIEIVPILKLdsplerefv------------- 122
gi 115421463  83 TYLDRLKRFAeqyys-------tsdikQSSP-SLVLQLn-------hIKFDLVPALKVwgttyqi--------------- 132
gi 156390715 161 KKLPDIQEQVsaclrnpppawkiedlwNSRF-AVQFKFkmkhwlhgeVEVDLLPTFQAdvesyeglflgrrklysqmhre 239
gi 4468185    90 PWLRRIYHFLannlkg------fkfeaLKNR-SLQFIYhhts--scvIKVDLLVSPYWescedfynflkkiped------ 154
gi 116242687  96 DILDKTGDKLkfclftkw--lknnfeiQKSLdGFTIQVftk---nqrISFEVLAAFNAls-------------------- 150
                        170       180       190
                 ....*....|....*....|....*....|....
Feature 1                           #   #  #       
1R8C_A       122 ---------------niksAVDRTpFHHKWLEGR 140
gi 86475969  143 -------rgkkysndenkkWEEADpINLKNKIMN 169
gi 117676292 143 ----------lnsnvenrvWSPAEpKKLIEHIND 166
gi 10957170  135 -----------nipynsssWLVTRpKAFDNLLVE 157
gi 83591054  163 ------------dpngdgwSKVIRpIKFAEKLTN 184
gi 41614948  123 ----------tdisqfhveWVKKNiKGLENDAKY 146
gi 115421463 133 -------------pngpaaWQNTNpNDFNGQLDA 153
gi 156390715 240 kgkdilkyysaaacklqrdFIKELpSNVKDLILL 273
gi 4468185   155 ----krtiftvcasrwqreFMSRQpPIVKNFIRQ 184
gi 116242687 151 ------------------lNDNPSpWIYRELKRS 166

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