1V4A


Conserved Protein Domain Family
NT_GlnE_GlnD_like

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cd05401: NT_GlnE_GlnD_like 
Click on image for an interactive view with Cn3D
Nucleotidyltransferase (NT) domain of Escherichia coli adenylyltransferase (GlnE), Escherichia coli uridylyl transferase (GlnD), and similar proteins.
Escherichia coli GlnD and -E participate in the Glutamine synthetase (GS)/Glutamate synthase (GOGAT) pathway for the assimilation of ammonium nitrogen. In nitrogen sufficiency, GlnE adenylates GS, reducing GS activity; when nitrogen is limiting, GlnE deadenylates GS-AMP, restoring GS activity. When nitrogen is limiting, GlnD uridylylates the nitrogen regulatory protein PII to PII-UTP, and in nitrogen sufficiency, it removes the modifying groups. The activity of Escherichia coli GlnE is modulated by PII-proteins. PII-UMP promotes GlnE deadenylation activity, and PII promotes GlnE adenylation activity. Escherichia coli GlnE has two separate NT domains. The N-terminal NT domain catalyzes the deadenylylation of GS, and the C-terminal NT domain the adenylylation reaction. The majority of proteins in this family contain a C-terminal NT domain which is associated with a cystathionine beta-synthase (CBS) domain pair and a CAP_ED (cAMP receptor protein effector ) domain. This family belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, coordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. For the majority of proteins in this family, these carboxylate residues are conserved.
Statistics
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PSSM-Id: 143391
View PSSM: cd05401
Aligned: 76 rows
Threshold Bit Score: 66.979
Threshold Setting Gi: 157165508
Created: 23-Jul-2007
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
metal binding
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1: metal binding triad
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1V4A_A        92 LFRRRIXVRIAWAQTLAlvte-------------esiLQQLSYLAETLIVAARDWLYdaccrewgtpcnaqgeaqPLLIL 158
gi 118475543   9 FLKSKDEDSVLASCKDGvlkckkalkkqfiklggrsfGTYQSKEIDKFFKCAFEATKisyfgef----apddeqlPISVI 84
gi 119025133   9 KQRFMAMSQPDADGVYRnga---------------akRKARTELAMGNLTQLWNEACeavsf--------dvpatGIGLG 65
gi 157165508  17 KEKFLDFKANLPKHFQKnqg--------------rnfTNFLAKEYDDFIKSYLNETMreffdef----vpqndsfAFSIL 78
gi 15610055    5 SPCAASDLAVARRELLSgnhrel---------dpvglRQTWLDLHESWLIDKADEIGiad-------------asGFAIV 62
gi 34556479   44 KGEVRHYMEGLEEKFDQnlg--------------rhfSYRHAREMDRLVAFVYKAVIrryfkey----lpplnhvPLVFI 105
gi 57833860    5 YGRTYGCRGLGTQRLRGgpaapppe----garsgpprRAALAELTDDWLTGLFDAGGag--------------prGVSLV 66
gi 148359259   9 KNTIKQFKEKLCKDFSQkani-------------tsiTRKLAVFIDTILIQLFIKNKlhf-------------gdNFCLL 62
gi 149178337  17 RTKVEQARQRGQELLRLga----------------sgLQIATAIAESIEQLLLQIIQdqfqqlpe--prqkllvqNCSIL 78
gi 81170616   50 QTTRENVLSILRRHLGSgnatvrrefe-krrmsgidaARALARQADDMVCALAELAAqkhe----------spgeTLCLC 118
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                      # #                                               #               
1V4A_A       159 GXGKLGGGELnfSSDIDLIFAwpehgctqggrreldnaqfftRXGQRLIKVldqptqdgfVYRVDXRLRPFgesgPLVLS 238
gi 118475543  85 ALGSYALSEMsvRSDIDFFISy-------------------kNVAGYNVKDivk---syiKFLKDINLDINpvyyEIDEL 142
gi 119025133  66 AVGSLARGQVgpSSDLDLAVIyepha------------ltdqQLNELANKLw--------YPIWDSGLDLDqsvrTVAQC 125
gi 157165508  79 ATGKYAQTLLsaNSELEILLVy-------------------kNLKGYNIKNflke-fseiLDSSGMKFVIKi--aEVDEI 136
gi 15610055   63 GVGGLGRRELlpYSDLDVLLLhdg---------------kpaDILRPVADRl-------wYPLWDANIRLDhsvrTVSEA 120
gi 34556479  106 ALGSYGREQLsvHSDIDLMIVykev--------------ggyNLLEMIEKMi--------HIGWDCGLKIShrvhEVGEL 163
gi 57833860   67 AVGGYGRGELspRSDLDLLLLhdgg--------------dqgAVAALADRIw--------YPVWDLGLALDhsvrTPAEA 124
gi 148359259  63 ALGSYGRRELqlHSDIDLLILhtek--------------vsnIQLQRAQKFi--------QDCWDVGLEVShqitTVSSC 120
gi 149178337  79 VIGGSGRGLMapYSDVDLLFLyrnq--------------vneEFAAFVGDIv--------RNCWDAGLKLGhslrTISDS 136
gi 81170616  119 ATGGYGAGLLapFSDIDILFLipgd--------------ptpAMTARIEFIl--------YALWDLGLRVGhatrSIAEC 176
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*
Feature 1                                                     
1V4A_A       239 FAALEDYYqeqgrdwerYAXVKARI-XGDsegvYANELRAXLRPF 282
gi 118475543 143 FDAIKDDLel------kTMFYQVRYvCASk--qLYKMTKDKIFEL 179
gi 119025133 126 EAVTDRDLpa------aMGWLDVVPiAGDt--gLIESTAVSILER 162
gi 157165508 137 FINFKDDLkf------kSETSQLRYiCGSk--sLYRLVKSEIIKI 173
gi 15610055  121 LTIANSDLma------aLGMLEARHiAGDq--qLSFALIDGVRRQ 157
gi 34556479  164 LEVSRSDIti------kTSFLEARFiCGSk--iLYMETEARIEQI 200
gi 57833860  125 RKTAGEDLkv------qLGLLDARHlAGDl--aLTAGLRTAVLAD 161
gi 148359259 121 ANLASQDLsv------iSTIMDMFLlCGHg--aLMEELIYQTHTL 157
gi 149178337 137 VKMARTEPef------aTAMIEARAiWGDe--hLAEQLIRSFYRH 173
gi 81170616  177 VRDADSDLti------rTALLDLRFlHGEr--gLARDLRCALGAD 213

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