1ZAP,2H6T,1J71,1EAG,2H6S


Conserved Protein Domain Family
SAP_like

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cd05474: SAP_like 
Click on image for an interactive view with Cn3D
SAPs, pepsin-like proteinases secreted from pathogens to degrade host proteins.
SAPs (Secreted aspartic proteinases) are secreted from a group of pathogenic fungi, predominantly Candida species. They are secreted from the pathogen to degrade host proteins. SAP is one of the most significant extracellular hydrolytic enzymes produced by C. albicans. SAP proteins, encoded by a family of 10 SAP genes. All 10 SAP genes of C. albicans encode preproenzymes, approximately 60 amino acid longer than the mature enzyme, which are processed when transported via the secretory pathway. The mature enzymes contain sequence motifs typical for all aspartyl proteinases, including the two conserved aspartate residues other active site and conserved cysteine residues implicated in the maintenance of the three-dimensional structure. Most Sap proteins contain putative N-glycosylation sites, but it remains to be determined which Sap proteins are glycosylated. This family of aspartate proteases is classified by MEROPS as the peptidase family A1 (pepsin A, clan AA). The overall structure of Sap protein conforms to the classical aspartic proteinase fold typified by pepsin. SAP is a bilobal enzyme, each lobe contributing a catalytic Asp residue, with an extended active site cleft localized between the two lobes of the molecule. One lobe may be evolved from the other through ancient gene-duplication event. More recently evolved enzymes have similar three-dimensional structures, however their amino acid sequences are more divergent except for the conserved catalytic site motif. This family of aspartate proteases is classified by MEROPS as the peptidase family A1 (pepsin A, clan AA).
Statistics
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PSSM-Id: 133141
View PSSM: cd05474
Aligned: 93 rows
Threshold Bit Score: 226.678
Threshold Setting Gi: 50550411
Created: 7-Sep-2006
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 2 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic residue [active site]
Evidence:
  • Comment:The two ASPs at the active site plays key catalytic roles in the pepsin family and conserved for all family members.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                           #                                                           
1ZAP_A       12 VTYAADITVGsnnqKLNVIVDTGSSDLWVPdvnidcqvtysdqtadfckqkgtydpsgssasqdlntPFSIGYGDgSSSQ 91
gi 50545607  59 YWYSVALQLGspaqNFNVLLDTGSSDLWVYasndtqdcennacaft-----gtfnadqsstyqflndDFSIHYVS-GSSV 132
gi 50547309  57 YYYSVALSLGtpaqDFNVILDTGSSDLWVFasnatqscenhacdft-----gqfnaldsssyhfignNYSIEYVSgDNAT 131
2H6T_A       12 VSYASDITVGsnkqKLTVVIDTGSSDLWVPdsqvscqagqgqdpnfck-negtyspssssssqnlnsPFSIEYGDgTTSQ 90
gi 50549149  60 QYYSIDLTLGtpaqKFNVLLDTGSSDLWVYnvsdttdcaggacknt-----gtfdssksstyhsiddKYFIQYVS-GNAS 133
gi 50548299  47 TYYRAKVSLGtpaqVFNVIFDTGSSDLWVPnydskass----------------------sykylnsDFNVSYTAvNGIL 104
gi 50553016  47 VYISTYISVGtppqSFEVSFDTGSSDLWIPavgddqtggpglf-----------npqasstfkslgtSFNAAYVG-GTAQ 114
gi 50548245  43 RYLYTNITLGtppqKFEVTFDTGSADLWVPtapdqqcsgqcpatag------afdadasstysllnnNFDVGYIGdPNNR 116
gi 50550399 409 LYYFVDAYLGtpgqKNSLLVDTGSSDLWVIdeangghfnkn----------------dsstwkhlnwDFKNAYQA-GQIS 471
gi 50554633  58 MAYNVDIKVGg--qKVTVQIDTGSSDLWVFssdsiycendpslctky----gsynpkksqnskdtgeHFDIQYGI-GDAS 130
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1ZAP_A       92 GTLyKDTVGFGgvsIKNQVLADvds-----tsiDQGILGVGYKtnea-----ggsYDNVPVTLKKQGVIAKNAYSLYLNs 161
gi 50545607 133 GNWgTDTLHIGgvsLTDFQFAAaas-----aggGQGVLGISLQgsesv--ppgnqYENFPLKLKSDGYIDRAVFSLYLDd 205
gi 50547309 132 GNWgTDTLSIGsvkLENLQFAAvth-----tsaQSGILGISLQgsesvv-fvkneYPNFPVQLVNQGWIDRTVYSLYLNs 205
2H6T_A       91 GTWyKDTIGFGgisITKQQFADvts-----tsvDQGILGIGYKthea-----egnYDNVPVTLKNQGIISKNAYSLYLNs 160
gi 50549149 134 GNWgTDTLSLEgvsLPDFRFATidn-----aqgNTGILGVSLPgsesvr-kgestYDNFPIALQKAGYIDRRVYSLYLNd 207
gi 50548299 105 GDWaTETLKLGpvtLENFQFGNvkg-----nhaGGGVFGVAFRaqemv--kpgdyYDNFPYAVKKAGYINKAAYSVFLDd 177
gi 50553016 115 GYWvTDNVNAAglsLSNLQLGAvsnm----qdqKQGILGVSFKqvemsvrngqpqYYNFPFSAKEQGFIDHVLYSLHFDg 190
gi 50548245 117 GNWvSDTLTVGsvtLPQFSFGTvq-------teDGGIFGVSFEeqessa-ddgetYPNFVFASKQQGYIDRAVFSIFSPs 188
gi 50550399 472 GEWgTDSVTIDnatVHDLVMGVantsaltsgdnVMGLLGLGFEglegtqqtdigqYKNYPRRLVDSGLTDVAAYSVWLNs 551
gi 50554633 131 GNYyKDDASLGeakVTDFQFGVstgd---kssgDVSVFGIGPLadea----trshYPNFPALLKEQGVIKKNVYGMAFGl 203
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                            #          
1ZAP_A      162 pd-saTGQIIFGGVDnAKYSGSLIALPVTSD--------RELRISLGSVEvsgkti----ntdnvDVLLDSGTTITYLQQ 228
gi 50545607 206 ls-ssTGNLLFGGVDkAKYEGDLAVLPLTSS--------AAFQVAYSGISvgqns------ygggNAVLDSGTSYTYIPD 270
gi 50547309 206 in-atEGTLLLGGIDtAKYSGHLEVLDLADH--------DDFSVPYSDVSwngns-----ygrasAAVFDSGTSYIYMPN 271
2H6T_A      161 rq-atSGQIIFGGVDnAKYSGTLIALPVTSD--------NELRIHLNTVKvagqs-----inadvDVLLDSGTTITYLQQ 226
gi 50549149 208 tn-afNGTFLLGGIDhAKFNGSLTVLPLPSP--------DEFAVKYENIIldgkp-----iakpgYTVLDSGTSFSYIPE 273
gi 50548299 178 pd-tnEATFLLAGVDhAKFEGDLSWLNVSDPa-------AGALVQLKDISlgskh-----mtvdaPVVLDTGTIFTSVPD 244
gi 50553016 191 ph-spDGTFLLGGIDhAKYSGDLHYYNVANPs-------AGPQINFKSLVldghe-----ldfnlPVTLDSGSLAIAFPD 257
gi 50548245 189 as-ssEVTFLLGGIDhAKFSGDLAWNKVPDPsrgasilvDDFSVNGVNFSly------------sSFAVDTGSVATLIPD 255
gi 50550399 552 rq-tgQGNFLFGGVDtAKYEGELQTIDMPDVvvg--nmrGYLTTHMTNIAinnpngsvsnlpkdlYAMFDTGAPSTLLPD 628
gi 50554633 204 pgdkqNSEITFGAYNsGRYSGSLKTVPITTQ--------GHFGIKCDKAKigdkt-----ilegeDVVLDSGTSLTYLTK 270
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1ZAP_A      229 DLADQIIKAFNgkltqdsn-gnsfyevDCNLsg--dVVFNFSKn------AKISVPASDFAastqgdd-gqpydkCQLLF 298
gi 50545607 271 QDFQQLAQDLNfqetdqs---tglpiiDCDSne--pVTFSFDG-------KDIVVPSSQMViplstlvgddsetqCVFGI 338
gi 50547309 272 DVFYGLSSDINlnettna--dtnlqyiACDAdi--nVVVTFGN-------TDITLGADQLVlhlsellddktqteCVFGI 340
2H6T_A      227 GVADQVISAFNgqetydan-gnlfylvDCNLsg--sVDFAFDKn------AKISVPASEFTaplyted-gqvydqCQLLF 296
gi 50549149 274 TVYNTIAKKHNlgdvtf----fglpsiDCKAnv--sLDFEFDG-------VTIHAQREQLIidpg-------igfCFFGV 333
gi 50548299 245 PIYQELKKTLNlgkfnkf---lginyiDCDAkm--sVAFNFPGatvladeKSLVVPIGPFYgnd--------dkrCGFGI 311
gi 50553016 258 TQFRAIGSGLNltnynkd---mglyyiDCQAei--sVDFKFDG-------LTISANSSSLVlpmgffsgdttdpsCVLGV 325
gi 50548245 256 DVFQHLTRTVQvgswipd---lgvytiDCNAep--sISFNLPNgtihahpETMVVPISRLSgnqs-------dtgCYFGL 323
gi 50550399 629 DVGLAFKDQFNlsynsa----wglyygDCNQgg--tFTFTLGG-------KDFTMDVKDFVvdwvsa--tipegqCFFGI 693
gi 50554633 271 SNYDAVINQVQaagikltdagqgisalPCSDigklsMEYTFSG-------KTIKVSGKDMTipatyldssdtsglCIFGI 343
                       330       340       350
                ....*....|....*....|....*....|....*...
Feature 1                                             
1ZAP_A      299 DVNk------ANILGDNFLRSAYIVYDLDDNEISIAQV 330
gi 50545607 339 QSAast--qdYVLFGDTFLRAAYVVYDLDQEQVGIAQA 374
gi 50547309 341 QSTdys--sdNILFGDVFLRSVYAVYDLDNYQIGVAQA 376
2H6T_A      297 GTSd------YNILGDNFLRSAYIVYDLDDNEISLAQV 328
gi 50549149 334 LPStys--qnITLFGDTFLRNAYVVYDLEDLQIGIAQA 369
gi 50548299 312 QSTtvf--ggDPIMGDTVMRAAYVVYDLEDHRCGVAQA 347
gi 50553016 326 QNSalyespnDAVLGEPFLKNAYVVYDLEDYSIGLAPA 363
gi 50548245 324 VSSety--dgISILGDSFLRNAYAVFDLEDYKLGFAQA 359
gi 50550399 694 GPTrg----kMAMFGDTFLRSFYAVFDLDNRKASLAKA 727
gi 50554633 344 TEStgq-lsnLNLFGDTFLRAIYSVYDLDNNQVSIAQA 380

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