Conserved Protein Domain Family
nucellin_like

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cd05475: nucellin_like 
Nucellins, plant aspartic proteases specifically expressed in nucellar cells during degradation.
Nucellins are important regulators of nucellar cell's progressive degradation after ovule fertilization. This degradation is a characteristic of programmed cell death. Nucellins are plant aspartic proteases specifically expressed in nucellar cells during degradation. The enzyme is characterized by having two aspartic protease catalytic site motifs, the Asp-Thr-Gly-Ser in the N-terminal and Asp-Ser-Gly-Ser in the C-terminal region, and two other regions nearly identical to two regions of plant aspartic proteases. Aspartic proteases are bilobal enzymes, each lobe contributing a catalytic Asp residue, with an extended active site cleft localized between the two lobes of the molecule. One lobe may be evolved from the other through ancient gene-duplication event. Although the three-dimensional structures of the two lobes are very similar, the amino acid sequences are more divergent, except for the conserved catalytic site motif.
Statistics
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PSSM-Id: 133142
View PSSM: cd05475
Aligned: 9 rows
Threshold Bit Score: 416.387
Threshold Setting Gi: 2290202
Created: 13-Feb-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
catalytic motifcatalyticActive site
Feature 1:catalytic motif [active site]
Evidence:
  • Comment:Catalytic motif in both N- and C-terminal lobes of this family.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                              ####                                                      
gi 21592493   58 GYYNVTINIGQPP--RPYYLDLDTGSDLTWLQCDAPCVRCleaphplyqpssdlipcnd----------plckalhlnsn 125
gi 162664943  29 GLYYMAMRIGNPA--KLYYLDMDTGSDLTWLQCDAPCRSCavgphglydpkrarvvdcrr--------ptcaqvqrggqf 98
gi 18402471  201 GLYYTRILVGKPEdgQYYHLDIDTGSELTWIQCDAPCTSCakganqlykprkdnlvrsse--------afcvevqrnqlt 272
gi 115448471 201 GQYYTSIFVGNPP--RPYFLDVDTGSDLTWIQCDAPCTNCakgphplykpakekivppk----------dllcqelqgnq 268
gi 157336057  60 GFYSVSLRIGNPP--KPYTLDIDSGSDLTWLQCDAPCVSCtkaphppykpnkgpitcnd---------pmcsalhwpskp 128
gi 15219354   47 GYYSVLMQIGSPP--KAFQFDIDTGSDLTWVQCDAPCSGCtlppnlqykpkgniipcsn---------pictalhwpnkp 115
gi 158513711  36 GHFFVTMNIGDPA--KPYFLDIDTGSTLTWLQCDYPCINCnkvphglykpelkyavkcte-------qrcadlyadlrkp 106
gi 12323376   65 GYYYVLLNIGNPP--KLFDLDIDTGSDLTWVQCDAPCNGCtkykpnhntlpcshilc--------------sgldlpqdr 128
gi 2290202    36 GHFYATLNIGEPA--KPYFLDVDTGSNLTWLECHHPVHGCkgchprpphpyytpadgnlkvvcgsplcvavrrdvpgipe 113
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 21592493  126 qrcetpeQCDYEVEYAdGGSSLGVLVRDVFSMnytqglrltpRLALGCGYDQipgas-shhpLDGVLGLGRGKVSILSQL 204
gi 162664943  99 tcsgdvrQCDYEVDYVdGSSTMGILVEDTITLvltngtrfqtRAVIGCGYDQqgtlakapavTDGVIGLSSSKISLPSQL 178
gi 18402471  273 ehcenchQCDYEIEYAdHSYSMGVLTKDKFHLklhngslaesDIVFGCGYDQqglllntllkTDGILGLSRAKISLPSQL 352
gi 115448471 269 nycetckQCDYEIEYAdRSSSMGVLARDDMHIittnggreklDFVFGCAYDQqgqllaspakTDGILGLSSAGISLPSQL 348
gi 157336057 129 pckasheQCDYEVSYAdHGSSLGVLVHDIFSLqltngtlaapRLAFGCGYDQsypgpnappfVDGVLGLGYGKSSIVTQL 208
gi 15219354  116 hcpnpqeQCDYEVKYAdQGSSMGALVTDQFPLklvngsfmqpPVAFGCGYDQsypsahpppaTAGVLGLGRGKIGLLTQL 195
gi 158513711 107 mkcgpknQCHYGIQYV-GGSSIGVLIVDSFSLpasng-tnptSIAFGCGYNQgknnhnvptpVNGILGLGRGKVTLLSQL 184
gi 12323376  129 pcadpedQCDYEIGYSdHASSIGALVTDEVPLklangsimnlRLTFGCGYDQqnpgphppppTAGILGLGRGKVGLSTQL 208
gi 2290202   114 csrndphRCHYEIQYV-TGKSEGDLATDIISVngr----dkkRIAFGCGYKQeepadsppspVDGILGLGMGKAGFAAQL 188
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 21592493  205 HSQGyv-kNVIGHCLss--lGGGILFFGDDLydssRVSWTPMsre----------yskHYSPAMggellfgg----rttg 267
gi 162664943 179 AAKGia-nNVIGHCLaggsnGGGYLFFGDTLvpalGMTWTPMigrp---------lveGYQARLrsikyggevlelegtt 248
gi 18402471  353 ASRGii-sNVVGHCLasdlnGEGYIFMGSDLvpshGMTWVPMlhdsrldayqmqvtkmSYGQGMlsldge---------n 422
gi 115448471 349 ANQGii-sNVFGHCItrdpnGGGYMFLGDDYvprwGMTSTPIrsapdnlf-hteaqkvYYGDQQlsmrga---------s 417
gi 157336057 209 RSLGli-rSIVGHCLsg--rGGGFLFLGDGLsttpGIIWTPMsrks---------gesAYALGPadllfngq-----nsg 271
gi 15219354  196 VSAGlt-rNVVGHCLss--kGGGFLFFGDNLvpsiGVAWTPLlsq-----------dnHYTTGPadllfngk-----ptg 256
gi 158513711 185 KSQGvitkHVLGHCIss--kGKGFLFFGDAKvptsGVTWSPMnre-----------hkHYSPRQgtlqfnsns---kpis 248
gi 12323376  209 KSLGit-kNVIVHCLsh--tGKGFLSIGDELvpssGVTWTSLatns---------pskNYMAGPaellfndk-----ttg 271
gi 2290202   189 KGHKmikeNVIGHCLss--kGKGVLYVGDFNpptrGVTWAPMres-----------lfYYSPGLaevfidkq----pirg 251
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                ####                                                                    
gi 21592493  268 lknlLTVFDSGSSYTYFNSKAYqavtyllkrelsgkplkeardd---htlplcwqgrrpfmsieevkkyFKPLALSFktg 344
gi 162664943 249 ddvgGAMFDSGTSFTYLVPNAYtavlsavvrqaqrsgleriktd---ttlpfcwrgpspfesvadvsayFKTVTLDFggs 325
gi 18402471  423 grvgKVLFDTGSSYTYFPNQAYsqlvtslqevsgleltrddsdet--lpicwraktnfpfsslsdvkkfFRPITLQIgsk 500
gi 115448471 418 gnsvQVIFDSGSSYTYLPDEIYknliaaikyaypnfvqdssdr-----tlplclatdfpvryledvkqlFKPLNLHFgkr 492
gi 157336057 272 vkglRLVFDSGSSYTYFNAQAYkttlslvrkylngklketade-----slpvcwrgakpfksifevknyFKPFALSFtka 346
gi 15219354  257 lkglKLIFDTGSSYTYFNSKAYqtiinligndlkvsplkvaked---ktlpicwkgakpfksvlevknfFKTITINFtng 333
gi 158513711 249 aapmEVIFDSGATYTYFALQPYhatlsvvkstlskeckfltevkekdraltvcwkgkdkirtidevkkcFRSLSLKFadg 328
gi 12323376  272 vkgiNVVFDSGSSYTYFNAEAYqaildlirkdlngkpltdtkdd---kslpvcwkgkkplksldevkkyFKTITLRFgnq 348
gi 2290202   252 nptfEAVFDSGSTYTHVPAQIYneivskvrgtlsessleevkgr----alplcwkgkkpfgsvndvknqFKALSLKItha 327
                        330       340       350       360       370       380       390
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
Feature 1                                                                                
gi 21592493  345 wrsk--tlfeipPEAYLIISmkGNVCLGILNGteig---lqnLNLIGDISMQDQMIIYDNekqSIGWMPVDC 411
gi 162664943 326 twwssgkllelsPEGYLIVStqGNVCLGVLDAsvas---levTNILGDISMRGYLVVYDNmreQIGWVRRNC 394
gi 18402471  501 wlii-srklliqPEDYLIISnkGNVCLGILDGssvh---dgsTIILGDISMRGHLIVYDNvkrRIGWMKSDC 568
gi 115448471 493 wfvm-prtftilPDNYLIISdkGNVCLGFLNGkdid---hgsTVIVGDNALRGKLVVYDNqqrQIGWTNSDC 560
gi 157336057 347 ksa----qlqlpPESYLIISkhGNACLGILNGsevg---lgdSNVIGDIAFQDKMVIYDNerqQIGWVPKDC 411
gi 15219354  334 rrn---tqlylaPELYLIVSktGNVCLGLLNGsevg---lqnSNVIGDISMQGLMMIYDNekqQLGWVSSDC 399
gi 158513711 329 dkk---atleipPEHYLIISqeGHVCLGILDGskehp-slagTNLIGGITMLDQMVIYDSersLLGWVNYQC 396
gi 12323376  349 kng---qlfqvpPESYLIITekGRVCLGILNGteig---legYNIIGDISFQGIMVIYDNekqRIGWISSDC 414
gi 2290202   328 rgt---nnldipPQNYLFVKedGETCLAILDAsldpvlkelnFILIGAVTMQDLFVIYDNekkQLGWVRAQC 396

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