1HRN,1SMR,2G24


Conserved Protein Domain Family
renin_like

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cd05487: renin_like 
Click on image for an interactive view with Cn3D
Renin stimulates production of angiotensin and thus affects blood pressure.
Renin, also known as angiotensinogenase, is a circulating enzyme that participates in the renin-angiotensin system that mediates extracellular volume, arterial vasoconstriction, and consequently mean arterial blood pressure. The enzyme is secreted by the kidneys from specialized juxtaglomerular cells in response to decreases in glomerular filtration rate (a consequence of low blood volume), diminished filtered sodium chloride and sympathetic nervous system innervation. The enzyme circulates in the blood stream and hydrolyzes angiotensinogen secreted from the liver into the peptide angiotensin I. Angiotensin I is further cleaved in the lungs by endothelial bound angiotensin converting enzyme (ACE) into angiotensin II, the final active peptide. Renin is a member of the aspartic protease family. Structurally, aspartic proteases are bilobal enzymes, each lobe contributing a catalytic Aspartate residue, with an extended active site cleft localized between the two lobes of the molecule. The N- and C-terminal domains, although structurally related by a 2-fold axis, have only limited sequence homology except the vicinity of the active site. This suggests that the enzymes evolved by an ancient duplication event. The active site is located at the groove formed by the two lobes, with an extended loop projecting over the cleft to form an 11-residue flap, which encloses substrates and inhibitors in the active site. Specificity is determined by nearest-neighbor hydrophobic residues surrounding the catalytic aspartates, and by three residues in the flap. The enzymes are mostly secreted from cells as inactive proenzymes that activate autocatalytically at acidic pH. This family of aspartate proteases is classified by MEROPS as the peptidase family A1 (pepsin A, clan AA).
Statistics
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PSSM-Id: 133154
View PSSM: cd05487
Aligned: 7 rows
Threshold Bit Score: 613.323
Threshold Setting Gi: 37787745
Created: 9-Jan-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 2 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic residue [active site]
Evidence:
  • Citation:PMID 7608971
  • Comment:The two ASPs at the active site plays key catalytic roles in the pepsin family and conserved for all family members.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                  #                                                     
1HRN_A         9 LTNYMDTQYYGEIGIGTPpQTFKVVFDTGSSNVWVPSSKCSRLYTACVYHKLFDASdSSSYKHNGTELTLRYSTGTVSGF 88
gi 37787745   68 LINYLDTQYFGEISIGSPaQMFNVVFDTGSANLWVPSHSCSPLYTACFTHNRYDASkSLTHIFNGTGFSIQYASGNVRGF 147
gi 109287598  71 LTNFRDTQYYGEISIGTPaQIFKVVFDTGSSNLWVPSHQCSPLYSACVSHNRYDSSeSSTYKPKGTKITLTYGQGYIEGF 150
1SMR_A         9 LTNYLNSQYYGEIGIGTPpQTFKVIFDTGSANLWVPSTKCSRLYLACGIHSLYESSdSSSYMENGDDFTIHYGSGRVKGF 88
gi 126306831  64 LTNYEDTQYYGEINIGSPpQTFKVVFDTGSSDFWVPSSQCDPLYTACEFHNRYDASkSSTYKMNGSNFIIHYASGRVKGF 143
gi 149597611  47 LTNYLDAQYFGEIGIGSPaQTFKVIFDTGSANLWVPSINCKPIHSACETHNLYDASqSQTYMENGTQIAISYVSGTVKGF 126
2G24_A         5 LTNYMDTQYYGEIGIGTPpQTFKVVFDTGSSNVWVPSSKCSRLYTACVYHKLFDASdSSSYKHNGTELTLRYSTGTVSGF 84
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1HRN_A        89 LSQDIITVGGITVTQMFGEVTEMPALPFMLAeFDGVVGMGFieqAIGRVTPIFDNIISQGVLKEDVFSFYYnrdse-nsq 167
gi 37787745  148 LSEDVVVVGGIPVVQVFAEATALPAIPFIFAkFDGVLGMGYpdvAIDGITPVFDRIMSQHVLKENVFSVYYsrdp---th 224
gi 109287598 151 LSQDIVRVADIPITQFFTEAIALPSIPFMYAhFDGVLGMGYpkqAIGGVIPVFDNIMSEKVLSENVFSVYYsrhs---es 227
1SMR_A        89 LSQDSVTVGGITVTQTFGEVTQLPLIPFMLAqFDGVLGMGFpaqAVGGVTPVFDHILSQGVLKEKVFSVYYnrgp----h 164
gi 126306831 144 LSQDILTIGEIKVTQVFGEVTALPLIPFGLAwFDGILGLGYpkrSMSGITPVFDNIMAEGVLKEDVFSIYYsrss----g 219
gi 149597611 127 LSQDLVTIGGIPVIQMFAEITTLPTSSFMYAkFDGVLGMGYpaqAIGGITPVFDHILTQHVLKEDVFSVYYsrnskndhm 206
2G24_A        85 LSQDIITVGGITVTQMFGEVTEMPALPFMLAeFDGVVGMGFieqAIGRVTPIFDNIISQGVLKEDVFSFYYnrdse-nsq 163
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                               #                        
1HRN_A       168 slGGQIVLGGSDPQHYeGNFHYINLIktgVWQIQMKGVSVgssTLLCEDGCLALVDTGASYISGSTSSIEKLMEALGAkk 247
gi 37787745  225 ipGGELVLGGTDPNYHtGPFHYINTKeqgKWEVIMKGVSVgadILFCKDGCTAVIDTGSSYITGPASSISILMKTIGAve 304
gi 109287598 228 ntGGEIILGGSDPSHYtGDFHYVSTSregYWHVDLKGVSIenkIALCHDGCTATIDTGTSFISGPASSISVLMETIGAtl 307
1SMR_A       165 llGGEVVLGGSDPQHYqGDFHYVSLSktdSWQITMKGVSVgssTLLCEEGCEVVVDTGSSFISAPTSSLKLIMQALGAke 244
gi 126306831 220 knGGELILGGSDPNYYqGTFHYINTSrphFWQIQMQGVAVksyVLSCEDGCPAVVDTGTSFITGPTDSIRGLMTAIGAee 299
gi 149597611 207 vpGGEIILGGRDPTYYqGDFYYLDVSkkgFWQVNMKGVSVdrtLQFCQEGCAAMVDTGATLITGPVKDVKHMMDILGAqk 286
2G24_A       164 slGGQIVLGGSDPQHYeGNFHYINLIktgVWQIQMKGVSVgssTLLCEDGCLALVDTGASYISGSTSSIEKLMEALGAkk 243
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1HRN_A       248 r-lfdYVVKCNEGPTLPDISFHLGGKEYTLTSADYVFqesysskklCTLAIHAMDIPPPTGPTWALGATFIRKFYTEFDR 326
gi 37787745  305 laeggYTVSCNVVRLLPTVAFHLGGQEYSLTDEDYILwqsefgediCTVTFKALDVPPPTGPVWILGANFIARYYTEFDR 384
gi 109287598 308 s-rgdYVIDCNQINLLPDISFHLGDMTYSLSSSTYVLkysd--eteCTVAFSAIDIPPPRGPLWLLGATFIKQYYIEFDR 384
1SMR_A       245 krlheYVVSCSQVPTLPDISFNLGGRAYTLSSTDYVLqypnrrdklCTVALHAMDIPPPTGPVWVLGATFIRKFYTEFDR 324
gi 126306831 300 d-ggeYLVKCDLASTLPDISFNFDGKDFTLQGSDYVLedenqsdqmCLVAINGLDVSPPTGPLWVLGATFIRKFYVEFDR 378
gi 149597611 287 iggnmYAVDCKEVAQLPDISFHLGGRVFPLSSSDYVLqdsdfddvlCPLAFKGVDVHPPLGPLWVLGASFIRRYYIEFDR 366
2G24_A       244 r-lfdYVVKCNEGPTLPDISFHLGGKEYTLTSADYVFqesysskklCTLAIHAMDIPPPTGPTWALGATFIRKFYTEFDR 322
                        330
                 ....*....|
Feature 1                  
1HRN_A       327 rNNRIGFALA 336
gi 37787745  385 gNNRIGFARA 394
gi 109287598 385 qNNRIGFATS 394
1SMR_A       325 hNNRIGFALA 334
gi 126306831 379 hNNRIGFALA 388
gi 149597611 367 qNNRIGFAMA 376
2G24_A       323 rNNRIGFALA 332

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