1T6G


Conserved Protein Domain Family
xylanase_inhibitor_I_like

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cd05489: xylanase_inhibitor_I_like 
Click on image for an interactive view with Cn3D
TAXI-I inhibits degradation of xylan in the cell wall.
Xylanase inhibitor-I (TAXI-I) is a member of potent TAXI-type inhibitors of fungal and bacterial family 11 xylanases. Plants developed a diverse battery of defense mechanisms in response to continual challenges by a broad spectrum of pathogenic microorganisms. Their defense arsenal includes inhibitors of cell wall-degrading enzymes, which hinder a possible invasion and colonization by antagonists. Xylanases of fungal and bacterial pathogens are the key enzymes in the degradation of xylan in the cell wall. Plants secrete proteins that inhibit these degradation glycosidases, including xylanase. Surprisingly, TAXI-I displays structural homology with the pepsin-like family of aspartic proteases but is proteolytically nonfunctional, because one or more residues of the essential catalytic triad are absent. The structure of the TAXI-inhibitor, Aspergillus niger xylanase I complex, illustrates the ability of tight binding and inhibition with subnanomolar affinity and indicates the importance of the C-terminal end for the differences in xylanase specificity among different TAXI-type inhibitors. This family also contains pepsin-like aspartic proteinases homologous to TAXI-I. Unlike TAXI-I, they have active site aspartates and are functionally active. This family of aspartate proteases is classified by MEROPS as the peptidase family A1 (pepsin A, clan AA).
Statistics
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PSSM-Id: 133156
View PSSM: cd05489
Aligned: 19 rows
Threshold Bit Score: 362.054
Threshold Setting Gi: 15239656
Created: 8-Feb-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
protein-protein
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:protein-protein interaction interface
Evidence:
  • Structure:1t6g_b, Aspergillus niger xylanase and Triticum aestivum Xylanase inhibitor I complex, contacts determineda at 3.5A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1T6G_B        16 YTIPFHDG------ASLVLDVAGPLVWSTCdggqp---paeipCSSPTCllanaypapg----cpapscgsdKHDKPCTA 82
gi 147821120  59 QKRTPLKP------VPLVLDVNGRSLWVDCesnylsstynapqCHSTQCsranlhdcr-----tcsaqtrpgCHNNTCGL 127
gi 118487589  46 YLQTPLKP------TKLLLDLGATYTWVNCdgytss-tyqhvpCNSSIAnllgayacld----lcdgppgpnCGNNSFLL 114
gi 87240526   56 HKRTPLMQ------VPVLLDLNGKHLWVNCeqhyasstyqapyCHSTQCsranahtch-----tcvssfrpgCHNNTCGL 124
gi 125552286  48 YVTMFRQRtpq-vpVKAVLDLAGTMLWVDCdagyvsssyagvrCGAKPCrllknagcai----tcldapsagCLNDTCSE 122
gi 125529029  26 SLYTVSVGg-----APLVVDLAGPLLWSTCppah-----rtvpCSSSVCkvanwyrspascpysdggrpgsgDRGCACAA 95
gi 21537233   49 NQRTPLVP------ASVVFDLGGRELWVDCdkgyvsstyqsprCNSAVCsragstscg-----tcfspprpgCSNNTCGG 117
gi 157347925  48 CLKTPLKP------SKLLLDLGGSFSWVDCykhyvsstyhhipCNSSLCtllslnsca-----hcyrapsptCANDTCAT 116
gi 14549156   52 QKRTPLMQ------VPVLVDLNGNHLWVNCeqqyssktyqapfCHSTQCsranthqcl-----scpaasrpgCHKNTCGL 120
gi 15238970   36 NTILPIFTftlntnQEFFIHIGGPYLVRKCndglp---rpivpCGSPVCaltrrftphq----cslpsnkiiNGVCACQA 108
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1T6G_B        83 YPYNPv------sGACAAGSLshTRFVANTTDGskpv----skvnVGVLAACAPs-kLLASLPRGSTGVAGLANSGLALP 151
gi 147821120 128 NAANPi------sGETAFGELaqDVLSIPSTDGsslgq---lvtiPQFLFACAPsslAQKGFPPAVQGVVGLGHTSIALP 198
gi 118487589 115 FPDNPikpvdykkVKGINIALi-DSFALSTTQGslt-------liNNFIFSCARt-gFLKGLAKGVAGLAALGRSNVSIP 185
gi 87240526  125 MSANPv------tQQTAMGELaqDVLAIYAINGpkpgp---mvtiPQFLFSCAPsflAQKGLPNNVQGVVGLAHSPISLQ 195
gi 125552286 123 FPKNTat-----sVSTAGNIIt-DVLSLPTTFRpapgp---lataPAFLFTCGHt-fLTQGLADGATGMVSLSRARFALP 192
gi 125529029  96 YPYNP--------VSGQCGRG--DVAAVPLAANatdgknplfpvsFSAFASCAPs-gLLASLPSGVAGVAGMSRLPLSLP 164
gi 21537233  118 IPDNTv------tGTATSGEFalDVVSIQSTNGsnpgr---vvkiPNLIFDCGAt-fLLKGLAKGTVGMAGMGRHNIGLP 187
gi 157347925 117 TLHNSv------tGKSIFHSAlvDAAALPTTDGrnpgr---lallANFAFACSTt-dLLKGLAKGVTGSAGLGWSDLSLP 186
gi 14549156  121 MSTNPi------tQQTGLGELgeDVLAIHATQGstqqlg-plvtvPQFLFSCAPsflVQKGLPRNTQGVAGLGHAPISLP 193
gi 15238970  109 TAFEPf------qRICNSDQFtyGDLSISSLKPisps-----vtiNNVYYLCIPq-pFLVDFPPGVFGLAGLAPTALATW 176
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1T6G_B       152 AQVASaqk-vanRFLLCLpt----ggPGVAIFGGGPvpwp-------------qftqSMPYTPLvtkg-----------g 202
gi 147821120 199 TQLAShfg-fqqKFALCLtsp---lnHGVLFLGEAPyrlhpg----------idvshPLGSTPLsis------------r 252
gi 118487589 186 VQFNRffssspnCFAICLsgs--ksqPGVALFGSKGpydflp---------gidlskSLLYTPLisnpfgkdsdpdkprs 254
gi 87240526  196 NQLSShfg-lkrQFTMCLsrh--pnsNGAILFGDAPnnmhfgqgnnynnknnpnlfnNLVYTPLtit------------q 260
gi 125552286 193 TQLADtfg-fsrKFALCLppa---saAGVVVFGDAPytfqpg----------vdlskSLIYTPLlinp----------ek 248
gi 125529029 165 SQVASslk-verQFALCLpasg-gggDGAAIFGGGPfqllaap--------pmeiteGLRRNPVpllkn---------pk 225
gi 21537233  188 SQFAAafs-fhrKFAVCLts-----gKGVAFFGNGPyvflp-----------giqisSLQTTPLlinpvstasafsqgek 250
gi 157347925 187 VQFIAgls-lprVFALCLsgs--psaPGVGFYGSAGpyhflp---------eidlskKLIYTPLlvnpygtaldsnhgrp 254
gi 14549156  194 NQLAShfg-lqrQFTTCLsry--ptsKGAIIFGDAPnnmrqfq--------nqdifhDLAFTPLtit------------l 250
gi 15238970  177 NQLTRprlglekKFALCLpsdenplkKGAIYFGGGPyklrn-----------idarsMLSYTRLitnp----------rk 235
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1T6G_B       203 spAHYISArSIVVGdtrvpvp-------egalatGGVMLSTRLPYVLLRPDVYRpLMDAFTKalaaqhangapvaravea 275
gi 147821120 253 egEYFIQVtSIRINervvpvnp-----allnrrpGSTLISTTTPYTVLEHSIYQtFTQFYANqmswaprvqpiapf---- 323
gi 118487589 255 spEYYIGLnSIKVNgkmvalnksl-laidgetgpGGTTISTVVPYTKLQRSIYKtFILAFLKeaaspafnltatkpvk-- 331
gi 87240526  261 qgEYRIHVtSIRLNqhtvvpvsapmlssypegvmGGTLISTSIPYTILQHSLFEaFTQVFAKqyprqaqvnav------- 333
gi 125552286 249 stDYFIGLtGIKVNghtvpvnat--llaidkkgvGGTKLSTVSPYTVLERSIHQaVTDAFAKemaaiprapavepf---- 322
gi 125529029 226 ngAYYLRVtGIAVNleavpippra-ldldvrrgtGGVTLSTVASYTTLRPDVYRaLLGAFDAatsgipraaavrpf---- 300
gi 21537233  251 ssEYFIGVtAIQIVektvpinptl-lkinastgfGGTKISSVNPYTVLESSIYNaFTSEFVKqalarsikrvasvkp--- 326
gi 157347925 255 sdEYFIGVtALKVNghavdlnpa--lltvdlngnGGTKISTVAPYTVLESSIYEaLTHAFIAesaglnltvhypvkp--- 329
gi 14549156  251 qgEYNVRVnSIRINqhsvfplnki-sstivgstsGGTMISTSTPHMVLQQSVYQaFTQVFAQqlpkqaqvksv------- 322
gi 15238970  236 lnNYFLGLkGISVNgnrilfapn--afafdrngdGGVTLSTIFPFTMLRSDIYRvFIEAFSQatsgiprvsst------- 306
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1T6G_B       276 vapfgvcydtktlgnnlgGYAVPNVQLGLdg--gSDWTMTGKNSMvdvk---qgtACVAFVEMKgvaag--dgrapAVIL 348
gi 147821120 324 ----glcfdatkmtatqiGPEVANIDLVLhn-rnNVWRIVGANSMvqpr---pgvWCLGFVDGGsnp-------kaPIIL 388
gi 118487589 332 --pfgvcypasavkntqmGPAVPIIDLVLdr-qdVVWKIFGSNSMvritkksvdlWCLGFVDAGvnpmvaswiggpSIVI 408
gi 87240526  334 -------gpfgmcfdskrINQALSVEFVMdr-pdVVWRISGENLMvqpr---ngvSCLAFVNGGlhp-------kaAITI 395
gi 125552286 323 ----klcydgrkxgstrvGPAVPTVELVLes-taASWVVFGANSMvatk---ggaLCLGVVDAGtep-------qtSVVI 387
gi 125529029 301 ----etcyrasalgttrlGFAVANIDLVLgg--gRNWTLPGGSSLvqvd---eqtVCFAFLEMMgtspa--aadspAIVI 369
gi 21537233  327 ---fgacfstknvgvtrlGYAVPEIELVLhs-kdVVWRIFGANSMvsvs---ddvICLGFVDGGvna-------rtSVVI 392
gi 157347925 330 ---frvcfpaddvmettvGPAVPTVDLVMqs-ddVFWRIFGRNSMvrileegvdvWCLGFVDGGvrp-------rtSIVI 398
gi 14549156  323 -------apfglcfnsnkINAYPSVDLVMdkpngPVWRISGEDLMvqaq---pgvTCLGVMNGGmqp-------raEITL 385
gi 15238970  307 -------tpfefclstttNFQVPRIDLELan--gVIWKLSPANAMkkvs---ddvACLAFVNGGdaa-------aqAVMI 367
                        410       420
                 ....*....|....*....|....*
Feature 1              #                  
1T6G_B       349 GGAQMEDFVLDFDMEkkRLGFSRLP 373
gi 147821120 389 GSYQLEDNLLQFDLArsKLGFSSSL 413
gi 118487589 409 GGHQLEDNMLQFDLQskRLGFSSSL 433
gi 87240526  396 GSRQLEENMMMFDLArsRLGFTNSL 420
gi 125552286 388 GGHMMEDNLLEFDLEasRLGFSSYL 412
gi 125529029 370 GGFQMENNLLLFDLEkgTLGISSLL 394
gi 21537233  393 GGFQLEDNLIEFDLAsnRFGFSSTL 417
gi 157347925 399 GGHQMEDNLLQFDLGlkRLGFSSSV 423
gi 14549156  386 GARQLEENLVVFDLArsRVGFSTSS 410
gi 15238970  368 GIHQMENTLVEFDVGrsAFGFSSSL 392

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