Conserved Protein Domain Family
PTS_IIB_galactitol

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cd05566: PTS_IIB_galactitol 
Click on image for an interactive view with Cn3D
PTS_IIB_galactitol: subunit IIB of enzyme II (EII) of the galactitol-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII is a galactitol-specific permease with two cytoplasmic domains (IIA and IIB) and a transmembrane channel IIC domain that are expressed on three distinct polypeptide chains, in contrast to other PTS sugar transporters. The three genes encoding these subunits (gatA, gatB, and gatC) comprise the gatCBA operon. Galactitol PTS permease takes up exogenous galactitol, releasing the phosphate ester into the cytoplasm in preparation for oxidation and further metabolism via a modified glycolytic pathway called the tagatose-6-phosphate glycolytic pathway. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include galactitol, chitobiose/lichenan, ascorbate, lactose, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system.
Statistics
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PSSM-Id: 99908
View PSSM: cd05566
Aligned: 40 rows
Threshold Bit Score: 83.3555
Threshold Setting Gi: 15900549
Created: 11-May-2007
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:most of the active site residues are contained within a P-loop which forms part of a binding pocket for the phosphoryl group
  • Comment:for the E coli chitobiose transporter IIB subunit, in addition to the P-loop residues, two conserved residues are likely to be important in catalysis: a tyrosine whose side-chain points towards the phosphorylation site and a Gln residue whose side-chain lies close to the sulfur atom of the catalytic cysteine.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1              # ##  ##                                                                  
1TVM_A        22 RKIIVACGGAVATSTMAAEEIKELCQSHNI-PVELIQCRVNEiet--ymDGVHLICTTARVdr------sfGDIPLVHGM 92
gi 89111015    2 KKILVACGTGMSTSTMIAHKLQEFLTEQGI-SATTAQCCLNEipl--ncNGMDLIVTSMRTns-------dYGIPTLNGA 71
gi 116871768   4 IKVISVCGSGTVTSSMIAGKVKDVLADNGF-RATTVEVNPGGveqelssGNWDIIVHTSPLrg-------kYDIPTINAV 75
gi 150015432   4 VNVLSVCGSGTVTSSMVAGKVKETLKEKGI-SSSTTEARPTEalnlaqsGRFDFIVYTSPLpp------gdYKIPTINAF 76
gi 160914637   2 KKVIVACGSGVATSQTVASKVNRLLKEKGV-DAVVEAVDLKSvdr--hlVDACAYITITKVsk-------eYDVPVINGI 71
gi 158437532   2 KRIIVACANGVATSQAVASKVNRLLKERKV-DAVVDAVNIKSldr--diKDCVAYITITKTvk-------eYPVPVINGI 71
gi 16804136    2 KKVIVACGSGVATSQTVASKVERILKEKGV-RATVEAVDIKSleq--hiKTSDVYVAITKAnk-------eFDIPTLNGI 71
gi 69246624    3 NRLIVACGSGVATSQTIASKIQSMLEDDGI-AFPVEAVDYKSiqn--elLTAGIYVYVAQPdeevleqakdLGIEVFPGI 79
gi 160914638   2 KRIIVACGNGVATSQAVAFRINRMLEKNHLyDVKVEAVNIRElhr--elKGAVAYVEVMKTde-------kCDIPKIDGL 72
gi 28379907    2 AKIIVACGSGVASSELVVKHITDFFEDHHItGVEVEATDFKKlpd--ilANYDVYVWIARPneeiqtivdrYGIASVNGL 79
                         90
                 ....*....|....*....
Feature 1                           
1TVM_A        93 PFVSGVGIEALQNKILTIL 111
gi 89111015   72 ALLTGINDDALKQQIKALL 90
gi 116871768  76 GFLTGMDEEGFIEELLETA 94
gi 150015432  77 ACLTGIGEDKFFEDVFKTV 95
gi 160914637  72 AFLTGMNQNKELERLIEAI 90
gi 158437532  72 AFLTGVRQEDELKKLIDAV 90
gi 16804136   72 AFLTGMGMDEETEKLLNAL 90
gi 69246624   80 PFLTGMGVEPIYDSIKELV 98
gi 160914638  73 VFLYGNANQKEEEFQRLLD 91
gi 28379907   80 PIFTGQSPETSYRDIITAL 98

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