Conserved Protein Domain Family
PTS_IIB_mannitol

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cd05567: PTS_IIB_mannitol 
Click on image for an interactive view with Cn3D
PTS_IIB_mannitol: subunit IIB of enzyme II (EII) of the mannitol-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII is a mannitol-specific permease with two cytoplasmic domains (IIA and IIB) and a transmembrane channel IIC domain. The IIA, IIB, and IIC domains are expressed from the mtlA gene as a single protein, also known as the mannitol PTS permease, the mtl transporter, or MtlA. MtlA is only functional as a dimer with the dimer contacts occuring between the IIC domains. MtlA takes up exogenous mannitol releasing the phosphate ester into the cytoplasm in preparation for oxidation to fructose-6-phosphate by the NAD-dependent mannitol-P dehydrogenase (MtlD). The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include mannitol, chitobiose/lichenan, ascorbate, lactose, galactitol, fructose, and a sensory system with similarity to the bacterial bgl system.
Statistics
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PSSM-Id: 99909
View PSSM: cd05567
Aligned: 27 rows
Threshold Bit Score: 88.7822
Threshold Setting Gi: 15829222
Created: 2-May-2007
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 9 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:the active site residues are contained within a P-loop structure

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1             #########                                                                  
1VKR_A        14 RKIIVACDAGMGSSAMGAGVLRKKIQDAGLsqISVTNSAINNLp---PDVDLVITHRDLTERamrqvPQAQHISLTNFLD 90
gi 158445184 371 RKIVFACDAGMGSSAMGATKFHNRIKAERPd-LTVTNSSVDTIp---ADADVVVCQRVLADRaakcaPQAQLVTLDNFLA 446
gi 15902400  498 DAVVVAYGKAQGTATMGCETIRAIFRNKNIr-IPVSTAKISELgefnSKNIMIVTTISLQAEvqqaaPNSQFLIVDSLVT 576
gi 28377160  524 EIVFVHHDGHLGTATMATSLMKDRVKKANKh-VSVKNLGIDELa--dNNSLLVVSSAEAAKNlktryTQVQVLITDDLLS 600
gi 29375042  370 QQILFVCDAGMGSSAMGASLLSRQLKAVNLe-MPVTYQSVHQMk--wQPKTLVVIQAELKQLaqkyvPEKDMVSVQNFLE 446
gi 29375046  499 NEILLVHDDRVGSATMGMKVLEEILDKEKIs-MPIRKININELt--qQTQALIVTKAELTEQarkkaPKATHLSVKSMVN 575
gi 50914317  497 DEVVFAYGENKGSATMGQATLVEIFKNRDVg-IPVSKVENHQLvafnSKNILVVTNIANQSVaqqfaPQAQLLVVDSLVT 575
gi 69245310  497 KEILLVHDDRSGSATMGMTVLKDILDKNHLt-IPLRKVNIQDLk--dEPDSLIVAKESLAEKvqmaaPQSIHLSVNSMVN 573
gi 69245315  368 HSVIFACDSGMGSSAMGAALLKKKLAAEKId-LSVGYSSIYELn--nQVNQLVIVQHELAGLarekvPQSQIYVLDHFLE 444
gi 126699952 385 KKIVFACDAGMGSSAMGASRFKNRIKNLDLn-IEITNSSVDNLp---DDTQIVVTHNTLVERvaknnSSVEIVSINNFLN 460
                         90
                 ....*....|.
Feature 1                   
1VKR_A        91 SGLYTSLTERL 101
gi 158445184 447 DPHLDALYNAL 457
gi 15902400  577 TPEYDKMAARM 587
gi 28377160  601 SPKYDKMVSEL 611
gi 29375042  447 IKSYYPQVLAK 457
gi 29375046  576 PQKYETVVSLL 586
gi 50914317  576 TPEYDKMIDRM 586
gi 69245310  574 PQKYESIAQYL 584
gi 69245315  445 TDILLSRIKEA 455
gi 126699952 461 DPNLDALFKRL 471

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