Conserved Protein Domain Family
PTS_IIB_bgl_like

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cd05568: PTS_IIB_bgl_like 
PTS_IIB_bgl_like: the PTS (phosphotransferase system) IIB domain of a family of sensory systems composed of a membrane-bound sugar-sensor (similar to BglF) and a transcription antiterminator (similar to BglG) which regulate expression of genes involved in sugar utilization. The domain architecture of the IIB-containing protein includes a region N-terminal to the IIB domain which is homologous to the BglG transcription antiterminator with an RNA-binding domain followed by two homologous domains, PRD1 and PRD2 (PTS Regulation Domains). C-terminal to the IIB domain is a domain similar to the PTS IIA domain. In this system, the BglG-like region and the IIB and IIA-like domains are all expressed together as a single multidomain protein. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include this sensory system with similarity to the bacterial bgl system, chitobiose/lichenan, ascorbate, lactose, galactitol, mannitol, and fructose systems.
Statistics
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PSSM-Id: 99910
View PSSM: cd05568
Aligned: 155 rows
Threshold Bit Score: 55.9727
Threshold Setting Gi: 26553899
Created: 11-May-2007
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Feature 1:active site [active site]
Evidence:
  • Comment:most of the active site residues are contained within a P-loop which forms part of a binding pocket for the phosphoryl group
  • Comment:for the E coli chitobiose transporter IIB subunit, in addition to the P-loop residues, two conserved residues are likely to be important in catalysis: a tyrosine whose side-chain points towards the phosphorylation site and a Gln residue whose side-chain lies close to the sulfur atom of the catalytic cysteine.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1              # ##  ##                                                                  
gi 26250310  436 KRVLLVCSTGIGTSHLLKSRILRAFPEWTIv-DVISAANLSqv---lpDNIELIISTINLptVTMPVAYVTAFFNDADIK 511
gi 157151385 393 NDIIIVCNEGISTSQMLNSRLQRVFAFNIL--GTFSKREALawq--qtHHADLIITTVDIpeARFPTIKVNALLLSEDIE 468
gi 29377474  396 MTAYFLFQGEPAWKAFLQQELAAYLGTRVKl-QAIEYVELSql---tlNEADIIISNFPLdhLDLPVFYLSLIPTKNELR 471
gi 160914801 402 IYCFFLCENYHQVKQYLYNKLRKILPDTIQl-IQGTFENLQn------QSVDLVITTDNIvhLPIPVIEVSPFLTLADID 474
gi 145953014 399 KNVVLVCGSGYGSSRLLEVQLNKVFHDKINilQCLSFNQFLas---elSDVDIIISTIPLnhDSIPIVLVDLKLLKKDIE 475
gi 16804821  404 QKVLIVCASGLGTSQLLESKVRKEFENTLEivGVYSFKEYEqs----gTMCDFVISTVPLrmKLHPFIQVSPFLTKADIR 479
gi 154483147 402 KNVILVCGSGHATTRMLEARLNVVFPDKINivKCVSYNEYSnytkenvKDIDFVITTVVLksNLLPSIMVDFALNNKDVE 481
gi 88854660  393 LTAALVCPNYHDLHVILRERIETVLGDELHl-RVVITRTDVdl---aeLTTDLVLTTIDLrsFADNVVLIQPFLTEADVD 468
gi 69246632  400 YQTVLVSEYSKAMATFLEARLNRELPELTIm-DLVEYKPELkrq--dfPSVDFILATLPFkhISIPVIEISPMITETDLA 476
gi 10956443  397 KKVLLVTGESESWAEYLAATLAKRFGDKIQisTVFFAKKSDh-----dVNADFIISTIPLdlGSTPIICINSIPTERDYT 471

                 ....*....
Feature 1                 
gi 26250310  512 RVTEMVITE 520
gi 157151385 469 RIQKISNQS 477
gi 29377474  472 RLAELTLHS 480
gi 160914801 475 KINKVISNL 483
gi 145953014 476 NISKSITNN 484
gi 16804821  480 NITALMKQD 488
gi 154483147 482 SINRYLSKL 490
gi 88854660  469 SIRKTIGRV 477
gi 69246632  477 YLTKYMLEH 485
gi 10956443  472 NIQYYLDLQ 480

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