Conserved Protein Domain Family
PTS_IIB_fructose

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cd05569: PTS_IIB_fructose 
Click on image for an interactive view with Cn3D
PTS_IIB_fructose: subunit IIB of enzyme II (EII) of the fructose-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII (also referred to as FruAB) is a fructose-specific permease made up of two proteins (FruA and FruB) each containing 3 domains. The FruA protein contains two tandem nonidentical IIB domains and a C-terminal IIC transmembrane domain. Both IIB domains of FruA are included in this alignment. The FruB protein (also referred to as diphosphoryl transfer protein) contains a IIA domain, a domain of unknown function, and an Hpr-like domain called FPr (fructose-inducible HPr). This familiy also includes the IIB domains of several fructose-like PTS permeases including the Frv permease encoded by the frvABXR operon, the Frw permease encoded by the frwACBD operon, the Frx permease encoded by the hrsA gene, and the Fry permease encoded by the fryABC (ypdDGH) operon. FruAB takes up exogenous fructose, releasing the 1-phosphate ester in to the cytoplasm in preparation for metabolism primarily via glycolysis. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include fructose, chitobiose/lichenan, ascorbate, lactose, galactitol, mannitol, and a sensory system with similarity to the bacterial bgl system.
Statistics
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PSSM-Id: 99911
View PSSM: cd05569
Aligned: 94 rows
Threshold Bit Score: 69.8235
Threshold Setting Gi: 120612886
Created: 15-May-2007
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:most of the active site residues are contained within a P-loop which forms part of a binding pocket for the phosphoryl group
  • Comment:for the E coli chitobiose transporter IIB subunit,(not included in this IIB subgroup) in addition to the P-loop residues, two conserved residues are likely to be important in catalysis: a tyrosine whose side-chain points towards the phosphorylation site and a Gln residue whose side-chain lies close to the sulfur atom of the catalytic cysteine.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1              # ##  ##                                                                  
2R48_A         5 LLAITSCPNGIAHTYXAAENLQKAADRLGVSIKVETQGGIgvENKLTEEEIREADAIIIAADRSVnk--dRFIGKKLLSV 82
gi 153888698  68 LLAILGADGRTTRAMLAGAALRHAARESGYKLDVDVRGPDt-ASVVDTAALATAKAVVWAGLSPDaa--aRDANPRTLEV 144
gi 131491      4 IVAVTAGAKGVAHTHLAAEALSATAQALGHQIRVERHSAEgvEAPLQGAEIAAADVVLIAADLRIed--vRFVTKPVYRT 81
gi 67153863  176 LLIVTACPSGMVTSVLCARLLEAAALRLGWTTRVEVHDPRaiGSPLAEEDIAAADLVVVVKTGELal--dRFAGKRLLQS 253
gi 117621519   3 AILVTACPAGIATSFLAAKRIEQQAKLAGWELTLDVGSSVqpRQVPSKEQIAAADLVLVVASAPVdl--aAYDGKRVYQG 80
gi 149376865   3 LIIVTACPQGVATSFLAARALERASQQKGWEARTDIRSPQqpLSSLPVDVIASADLVVAATGTPTdl--nAYAGKRLYQI 80
gi 83717371    4 LIGLVAAPARTAAPTFAAHALGRAAQARGIALALETRSALgvQTPLSADQIGEASAVLIAADDPAsideaPFAGKTIHRV 83
gi 77381025    3 LAIVTSCPNGMVTSVLCARLLDAAAQRQGWSTSVEVVDAAhpERELSAATIEAAEWVLLVATGPVdm--tRFIGKKVFRI 80
gi 83593305    4 FIAVVGGGERSTQALLVAEALRRAASGAGHRIDIEVRSDQgvVNALSDDAIVRARAVILIGTGDLdv--gRFPGLPKLET 81
gi 157407727   4 LLAVVGGGDLSTHAVLAAEALRKAAGRRNTPITLELRGKGasGNPLSDAAIAEARAVLLVGEGDLge--aRFGALHRARA 81
                         90
                 ....*....|....*...
Feature 1                          
2R48_A        83 gvqdgIRKPEELIQKALN 100
gi 153888698 145 tldevLADARAVLARVMH 162
gi 131491     82 staraVTQTAAVLAEALA 99
gi 67153863  254 tpadaLQDPQRFLEEAAA 271
gi 117621519  81 smdlaLQDPAALLEAAAS 98
gi 149376865  81 pvtaaLPDAAAVLDQAEK 98
gi 83717371   84 sideaIRDADAVLAHASG 101
gi 77381025   81 apaqaLQDVEDVLRRGAV 98
gi 83593305   82 tieavLADVGAVLGRAEG 99
gi 157407727  82 aiedvLTDVNAVFDRLGA 99

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