Conserved Protein Domain Family
GlmM

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cd05802: GlmM 
GlmM is a bacterial phosphoglucosamine mutase (PNGM) that belongs to the alpha-D-phosphohexomutase superfamily. It is required for the interconversion of glucosamine-6-phosphate and glucosamine-1-phosphate in the biosynthetic pathway of UDP-N-acetylglucosamine, an essential precursor to components of the cell envelope. In order to be active, GlmM must be phosphorylated, which can occur via autophosphorylation or by the Ser/Thr kinase StkP. GlmM functions in a classical ping-pong bi-bi mechanism with glucosamine-1,6-diphosphate as an intermediate. Other members of the alpha-D-phosphohexomutase superfamily include phosphoglucosamine mutase (PNGM), phosphoacetylglucosamine mutase (PAGM), the bacterial phosphomannomutase ManB, and the bifunctional phosphomannomutase/phosphoglucomutase (PMM/PGM). Each of these enzymes has four domains with a centrally located active site formed by four loops, one from each domain. All four domains are included in this alignment model.
Statistics
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PSSM-Id: 100095
View PSSM: cd05802
Aligned: 76 rows
Threshold Bit Score: 551.32
Threshold Setting Gi: 162447891
Created: 22-May-2007
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the structures of Candida albicans AGM1 (N-Acetylglucosamine-phosphate mutase) and Pseudomonas aeruginosa PMM/PGM bound to their substrates and divalent metal ions.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1          # #  #                                                                        
gi 401561      6 YFGTDGIRGRVgdapiTPDFVLKLGWAAGKVlarh--gsrKIIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPA 83
gi 33239723   20 FFGTDGIRGKSqt-flTNSLVSQIGYWCNHVllg----egPILIGQDSRASSERIASALAHGLATKNREIWLLGLCPTPA 94
gi 33860799    4 IFGTDGIRGIFdk-elTYSLAYKVGYALGVStkt----nnPILIGRDTRVSGYILIEAISRGINAAGKEFIYLGICPTPA 78
gi 145846184   4 YFGTDGFRGEAnv-vlTVEHAFKVGRYLGWYfgqd--hkaRIVIGKDTRRSSYMFEYALAAGLTASGADAYLLHVTTTPS 80
gi 139438688   3 YFGTDGFRGEAnv-glTVEHAYKIGRFVGWYyganrerkaRVIVGKDTRRSSYMFEAALVSGLVASGADAYMLHVIPTPG 81
gi 160914181   4 YFGTDGARGRAne-tlTLDMAVKIGQYLGWYygqk--rhaKILIGKDTRLSSDMFEMGLAAGATSTGASVYLLGVCPTPS 80
gi 162447891   5 YFGTDGIRGIAfe-klNSKLAFKLGQAIAKKfs-----psEIIIGQDTRLSSNMLAYGVAYGAALAGVNVKIAGVVSTPM 78
gi 84029234   42 LFGTDGIRGKVge-llSAPLALQIGFWAGVVlrnhadqlgPVILGQDSRNSSDMLAMALSAGLTAAGLEVWYLGLCPTPC 120
gi 33864090   19 SFGTDGIRGRVgt-llTPAFILQVGYWCGQVlpd----qgPVLIGMDSRSSGAMVASALTAGLTAAGREVWTLGLCPTPA 93
gi 160901521   4 LFGTDGIREVVne-klTVDLAMKLGNALANLfgs---eykKLYIARDTRNSGKVLEMALASGALVGGMNVESCGVLTTPG 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                          ###       #                                                   
gi 401561     84 VAYLTRTfraeAGIVISASHNPFYDNGIKFFSiDGTKLPDAVEEAIEAEMEKeiscv----dsaeLGKASRIVdAAGRYI 159
gi 33239723   95 VSHLIKKynasGGLMISASHNPPEDNGIKIFDkTGEKISLEKQIFIDNKLKRkvlip----ickdKDNCINRNdLLKDYK 170
gi 33860799   79 IPFLIKKekfsGGIMISASHNPPEYNGIKIFDnNGEKIKKKLENQIELILETannlk---lstykKTSIRENNnLFNIYT 155
gi 145846184  81 VSYVVRTedfdCGIMISASHNPFYDNGIKVINsAGHKLEAEVEEKIEAYIDGeigelp-latkekIGRTVDYAaGRNRYI 159
gi 139438688  82 VAHQTVEgcfdCGIMISASHNPFCDNGIKLVNsDGFKMDEDVLELIEDYIDGksevpl--atgnhIGATVDYMqGRNRYI 159
gi 160914181  81 VSYLIQKehfdCGIMVSASHNPFHDNGIKLFNgNGCKMNPEIEEKIEAYIDDnstedvvvakderIGSVIPWSeGLELYE 160
gi 162447891  79 IAYYSKVkd-iIGVMITASHNPYTDNGIKVIK-SGYKMLDEEELNLETYIDDnevl-----sstkLGSIEITHdVEDIYI 151
gi 84029234  121 VAYLTSMseaiGGVMISASHNPPEDNGIKIFGaNGGKLSQALQAEIEKGLRGnlpit---snvsnCGRHYSRWeLVKNYG 197
gi 33864090   94 VPGLIRKlgaaGGLMVSASHNPPEDNGIKVFGaDGAKLSPAKQGLIEAGLRGeaigdkgrptitsCGPAYQRNeLLSHYR 173
gi 160901521  80 LAFITKIek-sIGVVISASHNPPMYNGLKVFC-EGFKISDETEEKLEEKILKgllky---sdyrdIGRYIEDS-SKKEYV 153
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 401561    160 EFCKATFPnelslsELKIVVDCANGATyHIAPNVLRELGANVIAIGCEPNGvNINAEVGATDVRALQARVLaekaDLGIA 239
gi 33239723  171 NSLLNTVDkes-liDIPIVLDLCWGSAsSCGEKLFKALGANVISINAIPDGeKINVNCGSTHLEHIKKVVLesnaQMGFA 249
gi 33860799  156 EGLINTMGden-ldGMKIVLDTCYGSAtTCAASIFQKLGANVKVINNEQDGlKINLNCGSTCLDPIIKAIKennaDMGFS 234
gi 145846184 160 GHLISVATrs--fkDMRVGLDCSNGSSsAIAKSVFDALGAKTYVINNEPDGtNINTDCGSTHIHVLQEFVKekklDVGFA 237
gi 139438688 160 ASLIASANfs--lqGVKIGIDCANGSAsSVAKPVFDALGADVRVINAAPDGfNINVDCGSTHMERLQRHVVeqglDVGFA 237
gi 160914181 161 SWLKSIVPmd--lnGMHILLDLANGSAtSCAAETLAALGATVEVIHSAPNGiNINTKCGSTHPEELQRMMKegnyDAGFA 238
gi 162447891 152 NVYQDLNIpk---tSMKITYDSANGANyLISKKVIESFASHTYQIGNRPDGlNINLNVGSTHLESIINAVKsngsDIGLS 228
gi 84029234  198 EALKRPWQnkvnlqGMKVVLDLAWGAAvGLAPSVFAEMGAEVISLHNAADGdRINVNCGSTHLEMLQAAVQehnaDLGFA 277
gi 33864090  174 DALLASVLhqr-ldGVPIVLDLCWGAAtACGAEVFAALGADLTVLHGEPDGrRINVGCGSTQLEPLRRAVIergaIMGFA 252
gi 160901521 154 DYVVSLYKknlqcnDLKIAVDVANGAAgAIINDIFGELGLNYTVYQNAPDGfNINENCGSTSPQTLSKIVKeekyDFGIL 233
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         # # ##                                       #                    ###          
gi 401561    240 FDGDGDRVIMVDHEGNKVDGDQIMYIIAREglrqg-qlrgGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEK 318
gi 33239723  250 FDGDADRMIAIDGKGRVIDGDHSLYLWGSSlqdknmlpeqRLVTTVMSNLGLEKAWLNRGGKLTRTPVGDQHVHKAMLTN 329
gi 33860799  235 FDGDADRVIGVDSEGNIIDGDHILFLWGRElleeklltnnTIISTKMANLGFENTWKKIGGILHRTEVGDKFIFEAIKKK 314
gi 145846184 238 YDGDADRCIAVDENGKVIDGDLILYICGKYlmeqgrlegnTIVTTVMSNLGLYKACDKIGMKYEQTAVGDKYVYENMLKN 317
gi 139438688 238 YDGDADRCLAVDERGRVVDGDQILYVCGVYlnkhgrlsggTVVPTIASNFGLVKSLELAGLSAVTSGVGDRHVYAKMREG 317
gi 160914181 239 FDGDADRLIACDEDGNLINGDYTMFICGCYmkdngllkddVVVTTVMSNLGLYHAFKDKGIRYEQTAVGDKYVFECMNEH 318
gi 162447891 229 FDGDGDRLLVVDKDGTTFDGDYIVYIIAKYlkskgklkkdTVVLTQMSNPGMLKAFKTLGIKVLQTPVGDKYVSEEIMNH 308
gi 84029234  278 FDGDADRVLAVDPTGRPVNGDYILYLWGLYlkqqnqlpdnLIVSTVMANLGFEKAWQQQGGKLIRTAVGDQYVQAEMIKT 357
gi 33864090  253 FDGDADRMLALDGHGRVVDGDHVLYLWGSDlqdrqalpqqRLVATVMSNLGFERAWQQRGGVLERTPVGDQHVYAAMVES 332
gi 160901521 234 FDGDADRCLFIDRFGNLVDGDVLMAINALKlkaqerlknkIVVSTIMSNLGLEEYLKKNEIHLIRTDVGDKYVLEKMLQE 313
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1              # # #                                                                     
gi 401561    319 GWRIGAENSGHVILLDKTTTGDGIVAGLQVLAAMArnhmSLHDLCSGM-KMFPQILVNVRYta--gSGDPLEHESVkavt 395
gi 33239723  330 KASLGGEQSGHILSTLNDLCGDGLLAAIQLSSICNrkgiLLSEWRDQSfKPYPQKLISVPIakhitQNYLNKSEKFrlsi 409
gi 33860799  315 KALLGGEQSGHILSKINDFCGDGILTAIQISKYCKkkniSLRSWLNSSfSPYPQKLTNVLLnf-kfKNIDKSYKEFinet 393
gi 145846184 318 GYILGGEQSGHIIFSKHARTGDGILTSLLIMEVILekkqSLGTLAGEV-KIYPQLLKNVRVt---dKKTARENPAVqeav 393
gi 139438688 318 GYSLGGEQTGHTIFGDIERTGDGIMTSLRVMEVIRaereTLSQLTAPC-RLLPQAQVAVHVa---dKDAALENMGVqnai 393
gi 160914181 319 GYSIGGEQSGHIIFHAHANTGDGLLTAMKLLEIMKntgqSLKQLGADL-FIYPQLLINTPVk---dKHAALEDAELlaav 394
gi 162447891 309 DLSIGGENSGHIIINDLLPSGDGLFAGVYILKILEehktSLKDYTKEV-EMYPQKMVNIKNv----DKEVLKHPEIiklv 383
gi 84029234  358 GAMLGGEQSGHILCSHYGMTGDGLLTALHLASLVKqsgvSLAELIDQSfQTYPQLLRNVRVvdrdrRLSWQNCTPVqqai 437
gi 33864090  333 NAALGGEQSGHILSAAHGLCGDGVLTALQLATLCHgrglSLGEWLDQSfQAFPQKLVNVRVpdlerRMGWQHCEPLqeav 412
gi 160901521 314 NATIGGEQSGHIIFLDRSTTGDGIITALETLETLKyfskSLDNFLQEI-PKYPQHLENVTVk---dKVKIMKDSRIenli 389
                        410       420       430       440
                 ....*....|....*....|....*....|....*....|....*
Feature 1                        # ###    #                   
gi 401561    396 aEVEAALGn-rGRVLLRKSGTEPLIRVMVEGeDEAQVTEFAHRIA 439
gi 33239723  410 aEAELDLGk-eGRVFIRKSGTEPLVRVMVESiDKLLVESLTTKIA 453
gi 33860799  394 iESFSNIKkdnCRVYIRPSGTEPVLRILVEArNQQEVDSLSTKIT 438
gi 145846184 394 qKAADELGs-dGRILVRESGTEPVIRVMVEAaTDEICEKYVNSVV 437
gi 139438688 394 aEAEAALAg-eGRVLVRKSGTESVIRVLAEApDQASADAAMKRIA 437
gi 160914181 395 dEVAKELGd-eGRILVRPSGTEPLVRVMVEAkTDALCEKYVYRIV 438
gi 162447891 384 eEVRRNLSe-dSLLLVRPSGTEPLVRVTISCqDILELDKYMGLLV 427
gi 84029234  438 aLAEKAMGd-tGRILVRASGTEPVIRVMVEAaNAELANYWTNELV 481
gi 33864090  413 lAAEAAMGe-dGRVLVRASGTEPLLRVMIEAaDSAAVEFWTAQLA 456
gi 160901521 390 rKYQSIEG---FRIVVRPSGTEPKIRIMTEGsNEETIKTCVTEFS 431

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