Conserved Protein Domain Family
CBM20_genethonin_1

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cd05813: CBM20_genethonin_1 
Genethonin-1, C-terminal CBM20 (carbohydrate-binding module, family 20) domain. Genethonin-1 is a human skeletal muscle protein with no known function. It contains a C-terminal CBM20 domain. The CBM20 domain is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch.
Statistics
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PSSM-Id: 99887
View PSSM: cd05813
Aligned: 5 rows
Threshold Bit Score: 168.83
Threshold Setting Gi: 47213312
Created: 11-Sep-2007
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
starch bindingstarch binding
Feature 1:starch binding site 1 [chemical binding site]
Evidence:
  • Comment:Based on the binding of substrates to the starch binding site 1 of other family members.
  • Comment:Site 1 is small relative to site 2 and exhibits minimal structural changes upon ligand binding. It acts as an initial starch recognition site.
  • Citation:PMID 9195884
  • Citation:PMID 8672460

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                     #                            #           ##   #    
gi 4503977   264 VSVRFQVHYVTSTDVQFIAVTGDHECLGRWNTYIPLHYNKDGFWSHSIFLPADTVVEWKFVLVENGGVTRWEECSNRFLe 343
gi 47213312   79 VNVTFRVQYHTQWPHQTVAVTGDLPELGSWKGFIPLEKVEEGQWSAVVSLPTESHVEWKFVLLDKGQVCRWEECRNRLLd 158
gi 125849062 437 VSVTFRIHYITHSALQLVAVTGSLQELGAWESFIPLQRAKDGFWANTVSLPVESQVEWKFVLVEDGRISRWEECGNRYLc 516
gi 118090258 168 IHVTFRVHYITHSGAQLIGVTGDHECLGQWHSYVPLKYDKDGFWSESVTLPVDTKVEWKFILVENGKVTRWEECSNRTLv 247
gi 126330900 257 VNVQFQVHYVTMSDASLVAVTGDHETLGGWHSYIPLQCGKDWFWSRSVPLPIDTTLKWKFVVVENGSIIRWEECCNRILe 336
                         90
                 ....*....|....*..
Feature 1                         
gi 4503977   344 -TGH-EDKVVHAWWGIH 358
gi 47213312  159 -TGLgDDMLVHTWWGLA 174
gi 125849062 517 lTSQdEEVFLEKCWGCC 533
gi 118090258 248 -TEH-EDQIAHQWWGYH 262
gi 126330900 337 -TGH-EDKIVYKWWGCH 351

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