Conserved Protein Domain Family
CBM20_Prei4

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cd05814: CBM20_Prei4 
Prei4, N-terminal CBM20 (carbohydrate-binding module, family 20) domain. Preimplantation protein 4 (Prei4) is a protein of unknown function that is expressed during mouse preimplantation embryogenesis. In addition to the N-terminal CBM20 domain, Prei4 contains a C-terminal glycerophosphoryl diester phosphodiesterase (GDPD) domain. The CBM20 domain is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch.
Statistics
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PSSM-Id: 99888
View PSSM: cd05814
Aligned: 14 rows
Threshold Bit Score: 138.225
Threshold Setting Gi: 19922666
Created: 11-Sep-2007
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
starch bindingstarch binding
Feature 1:starch binding site 1 [chemical binding site]
Evidence:
  • Comment:Based on the binding of substrates to the starch binding site 1 of other family members.
  • Comment:Site 1 is small relative to site 2 and exhibits minimal structural changes upon ligand binding. It acts as an initial starch recognition site.
  • Citation:PMID 9195884
  • Citation:PMID 8672460

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                             #                                          
gi 25901062    4 SQVAFEIRg---------tLLPGEVFAICGSCDALGNWNPQNAVALLPend----------------------------- 45
gi 19920660   44 RLWTFRVLmn-------qdLAPNEQLAIVGNCEALGNWQHSGAAILSKnee----------------------------- 87
gi 24666575   49 CKPTLREFnvrl----evpLAAEERLGLTGDVKALGEWQLSRSVALESl------------------------------- 93
gi 24666571   69 CVPLFRIFslelr--hnirLAPDEFVAVSGDHPALGLWQLEKALPLKEqd------------------------------ 116
gi 91082949   38 KRWLFRVRap--------dLKKDEVMCVSGSVPELGSWQAEKCVPLERe------------------------------- 78
gi 19527767   40 TEWPFCVVfh-------ssLNGNEYVAISGNCPSLGNWDPKEVYILAKndci---------------------------- 84
gi 19922666   47 KRNAPLVFnftltlpdadqLASFERPALVGNLPVLGAWQAGRAVLLNRt------------------------------- 95
gi 156553395   6 RDWMFRVQvp--------gLEKNDVVFVVGNLPELGAWNHNQAIQLTKehsgtdeaspaafenngssdftdfygnscdga 77
gi 71994766    7 VRVHFAVDve--------nLHAHQSVYVVGSNDVLGTWEATRAMPLVQdp------------------------------ 48
gi 71993742   98 YKAHFKVFcs--------eVREWEKIHVVGSLPVLGEWNPRKSFPLTKst------------------------------ 139
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                         #                        ##    #               
gi 25901062   46 -----------tgESMLWKATIVLSr-GVSVQYRYFKGYfle--pktIGGPCQVIvhKWETHLQPRSITPLe-------- 103
gi 19920660   88 ----------dneYSNLWTGEIYIPr-HCDTEYRYMVCAv-------DPGTEQLIvrRWETQLQPRIVKELd-------- 141
gi 24666575   94 -------------DELNWQATVALQs-CRQLEYRYFVYVe------dLSGYKQIR--RWETHFKPRSLGPCt-------- 143
gi 24666571  117 ------------kARTKWYLRVWICa-SQRFYYRYLIYSk-------NRQGNRVLr-TWEGQRVARMLQTYe-------- 167
gi 91082949   79 ------------dESDVWSLPVMIPd-RVNIQYRFCVCVv-------IDAGLQVIvrSWEAQIAPRAIHAT--------- 129
gi 19527767   85 ---------sclcNCRQFEASLEIPr-NIDIHYRYCVVIh-------DPETDEVYirFWESQLYPRVIRTC--------- 138
gi 19922666   96 ------------aILNVWSASVEIPq-NSSVEYRYFAAAv------gQSGAVQIR--RWESHVQARTFNTT--------- 145
gi 156553395  78 dsvgdcsaenseeGGRIFSQNIALPs-DADVQFRYFVAVvcepngtkNSAKTLIIr-KWETHMSPRQIKKDvpsnicaaa 155
gi 71994766   49 ------------dRFMRWKGSIVTD--VHQLKFRYFIGYnl-----mSDQGERLIvdKWEAFLHPRSTLCLaes-----r 104
gi 71993742  140 ------------dEENTFHAIVSVPssIKEFNFRYIRVMyldpsennTSEPIMVLs-KWETFNNARTCLIGves-----q 201
                        170       180       190
                 ....*....|....*....|....*....|.
Feature 1                                       
gi 25901062  104 --SEIIIDDGQFGi-----hNGVETLDSGWL 127
gi 19920660  142 -eQPAKNQSDIFGs-----fNGQEKVDRGWL 166
gi 24666575  144 --ELQCSQLDVFGits-dnsDLKPQVHRGWL 171
gi 24666571  168 --IYRSPGLDIFGeaypvsvGGGFYLERGWL 196
gi 91082949  130 --EKSPLETDEPLtyg--dyGNVCRIDRGWL 156
gi 19527767  139 --QNMLKNCDVFGkph--ddDEANQVDRGWA 165
gi 19922666  146 --KFSGNRSDEFGl-----iGNERQLSRGWL 169
gi 156553395 156 agEESLPDPDKFGy-----hNGYRKIERGWL 181
gi 71994766  105 ndECRVDRVDLFGy-----yAGRKCVSDGWL 130
gi 71993742  202 dgVARKNITDQFGs-----ySGKTQITDGWI 227

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