Conserved Protein Domain Family
CBM20_DPE2_repeat2

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cd05816: CBM20_DPE2_repeat2 
Disproportionating enzyme 2 (DPE2), N-terminal CBM20 (carbohydrate-binding module, family 20) domain, repeat 2. DPE2 is a transglucosidase that is essential for the cytosolic metabolism of maltose in plant leaves at night. Maltose is an intermediate on the pathway from starch to sucrose and DPE2 is thought to metabolize the maltose that is exported from the chloroplast. DPE2 has two N-terminal CBM20 domains as well as a C-terminal amylomaltase (4-alpha-glucanotransferase) catalytic domain. DPE1, the plastid version of this enzyme, has a transglucosidase domain that is similar to that of DPE2 but lacks the N-terminal CBM20 domains. Included in this group are PDE2-like proteins from Dictyostelium, Entamoeba, and Bacteroides. The CBM20 domain is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch.
Statistics
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PSSM-Id: 99890
View PSSM: cd05816
Aligned: 16 rows
Threshold Bit Score: 110.879
Threshold Setting Gi: 162669392
Created: 14-Jan-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
starch bindingstarch binding
Feature 1:starch binding site 1 [chemical binding site]
Evidence:
  • Comment:Based on the binding of substrates to the starch binding site 1 of other family members.
  • Comment:Site 1 is small relative to site 2 and exhibits minimal structural changes upon ligand binding. It acts as an initial starch recognition site.
  • Citation:PMID 9195884
  • Citation:PMID 8672460

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                      #                               #             ##   
gi 41176619   154 ILQFRICCp-YLEEGTSIYVLGSSLNLGQWKiqDGLKLVYAGDSLWQAACVMGKDDFPLKYKYCKHska---gtSVECGa 229
gi 123404859  156 RVYFSANCp-YVRAHQDVYISGSVPELGNWDakKGVKLADGKFPIWSGYVDFEKTSFPFDYKYVIAnkd--gtfIWETDg 232
gi 123439946  151 QTFFSTNCp-YVRPKQVVRIVGNVPELGNWNpkNGLVMRDGCFPIWTANKEFAKSSFPFEYKYVIEnted-gtfLWEDCe 228
gi 29347556   126 GLVIKAYAp-RINKDYCLAICGNQKALGNWDpdKAIPMSDANFPEWQIELDASKLKFPLEYKFILYhkeekkadCWENNp 204
gi 154414934  173 QVRFRLLVl-QLPPNYRVYITGDSSVFENWK--KYVPLVPINNLYWEIAIDMRNDTRPFDYKYLILkdgd-stpKWEHRp 248
gi 123413801  251 KTHFQVNCp-YVHSTQSVVVVGSIPELGEWNplHGLKLEDGKFPIWANSIDLKATSFPFEYKYVLSnnd--gtfIWEEQn 327
gi 150003954  127 SITFRALCpcLHHKRQVLAISGDCPALGNWDiqKTVLMEEIQPNEWTITLNVSTLEFPLSYKFVACnadskqveEWENHd 206
gi 150006677  131 KLVIKISAp-RVEKNQCVAITGNQECLGNWHpdKALLLSCDTFPEWHIDLDAAEIRYPLEYKFLVWdndsrqplYWESDe 209
gi 66809917   141 IIHFQVKSt-YVPPNHSIYIVGSNTQLGNWRtdRAVKLSDESYPIWKVDLEFSKDQLPFSYKYIIAdkik-svvHWEYGn 218
gi 67479419   115 VVILRCKWq-SVPSGYSIYVTGSSSEFGKWDtyKALQMKAVGMSWWELSIPIENIREAFEYKYFMKkt----evVWEDGp 189
                          90       100       110
                  ....*....|....*....|....*....|...
Feature 1         #                                
gi 41176619   230 SREIs-----VDVt---TGEs----RFVVLSDG 250
gi 123404859  233 NRHCp-----APVsv-sKYP-----LIDYVQEW 254
gi 123439946  229 NRVCp-----APVs---VNEn----LIDYVQDW 249
gi 29347556   205 NRYLa-----DPE----LKTn----ETLVISDR 224
gi 154414934  249 NHKFy-----FPF----ENP-----SVLIISDW 267
gi 123413801  328 NRHCh-----GPSl--sQYDgt-ipMTLFVNEW 352
gi 150003954  207 NRMLn-----NPEl--kKGEi---yLTPETEVH 229
gi 150006677  210 NRILs----lVPQk---QGEtv-viSGLYFRDS 234
gi 66809917   219 DRWFtasathASEysstTNEd----RDFFFNDG 247
gi 67479419   190 NRLF------APM----LGPfigckQAVIYQED 212

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