Conserved Protein Domain Family
CBM20_DSP

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cd05817: CBM20_DSP 
Dual-specificity phosphatase (DSP), N-terminal CBM20 (carbohydrate-binding module, family 20) domain. This CBM20 domain is located at the N-terminus of a protein tyrosine phosphatase of unknown function found in slime molds and ciliated protozoans. The CBM20 domain is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch.
Statistics
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PSSM-Id: 99891
View PSSM: cd05817
Aligned: 6 rows
Threshold Bit Score: 147.621
Threshold Setting Gi: 145478487
Created: 22-Jan-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
starch bindingstarch binding
Feature 1:starch binding site 1 [chemical binding site]
Evidence:
  • Comment:Based on the binding of substrates to the starch binding site 1 of other family members.
  • Comment:Site 1 is small relative to site 2 and exhibits minimal structural changes upon ligand binding. It acts as an initial starch recognition site.
  • Citation:PMID 9195884
  • Citation:PMID 8672460

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                   #                                  #               ##
gi 146185619  20 QVAFSVRCPTYFGQAVYISGNCDQLGNWNTEKATRMNWSEGNLWWCDISLpt----dVEVEYKYYVAdyedm-tnevCWE 94
gi 66824037   14 NVTFTIVYNTQPGEILFITGSMNQLGEWDTSKAKRMTWNIGNLWDASVGFpe----sVYIQYKYFVYnein---ktyRWE 86
gi 145552336   1 MLTFQIQYPLAFGEAMYMCGNFEQLGNWNPVQAKRMQWCEGDNWVLTVDIgvaaaeeQDFEYKYFFGefdr--ptniEWE 78
gi 145478487  18 NVYFKIHYHTQPGKAIYIVGDCNILGNWVSTKGVRLQWNENDEWTVCVKIdrs--qyVKIEYKFIVNnydyptlndtLWE 95
gi 145525537   1 MLSFQLHYQLQFGDAIYISGDSDYFGNWSPLQAKRMKWNQGHLWTIDIPI-------HYFQYKYFVSsfnd--pnseEWE 71
gi 145526611   1 MIHLKIQFKTEFGQAVYISGNSKYLGQWNPKQAIRMNWTENDIWEVEIAY-------HKIEYKYFISqyes--iqtvLWE 71
                         90       100       110
                 ....*....|....*....|....*....|....*..
Feature 1           #                                 
gi 146185619  95 QISNRVFr-------SQECq-------TKEIQDVFNH 117
gi 66824037   87 SIENRQLiv-----nNDELl------iEDAWDVKRNL 112
gi 145552336  79 MGPNRIVrt----teNTIItd-----iWNHRKILFQC 106
gi 145478487  96 PGENRVIt------nHMIE--------NETKSEYFNC 118
gi 145525537  72 FGPNRLMrttepscnHQLNlekgivdvWNHRKVMFQC 108
gi 145526611  72 SGPNRVTt------kHTIDv-------WNYRKVCFQC 95

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