Conserved Protein Domain Family
CBM20_water_dikinase

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cd05818: CBM20_water_dikinase 
Phosphoglucan water dikinase (also known as alpha-glucan water dikinase), N-terminal CBM20 (carbohydrate-binding module, family 20) domain. This domain is found in the chloroplast-encoded phosphoglucan water dikinase, one of two enzymes involved in the phosphorylation of plant starches. In addition to the CBM20 domain, phosphoglucan water dikinase contains a C-terminal pyruvate binding domain. The CBM20 domain is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch.
Statistics
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PSSM-Id: 99892
View PSSM: cd05818
Aligned: 5 rows
Threshold Bit Score: 176.155
Threshold Setting Gi: 125579082
Created: 18-Jan-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
starch bindingstarch binding
Feature 1:starch binding site 1 [chemical binding site]
Evidence:
  • Comment:Based on the binding of substrates to the starch binding site 1 of other family members.
  • Comment:Site 1 is small relative to site 2 and exhibits minimal structural changes upon ligand binding. It acts as an initial starch recognition site.
  • Citation:PMID 9195884
  • Citation:PMID 8672460

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                      #                           #              ##   #  
gi 79521972    73 KVRLNVRLDHQVNFGDHVAMFGSAKEIGSWKKKSPLNWSENGWVCELELDGGQVLEYKFVIV---KNDGslSWESGDNRV 149
gi 157353332    9 KVKLSILLKHQVKFGEHVVMLGSTKELGSWKKNVPMNWTENGWVCKLELRGDESIEYKFVIV---KRDKsmTWEGANNRV 85
gi 125579082   36 LVHLQVCLEHQVKFGEHVGIIGSTKELGSWEEQVELEWTTNGWVCQLKLPGETLVEFKFVIFlvgGKDK--IWEDGNNRV 113
gi 162696562   12 KVKVRVRLDHQVQFGETHAILGSAKSIGAWQEKVPMTWTEAGWVVDLEGDSGETVEYKHIIV---THDGgvIWEEGPNRT 88
gi 116792294   83 KVQLHFRLDHQVRFGENHAILGSSKALGAWKKKLMMSWSESGWILDLETGGGEKVEYKFLIV---SRDGkiIWEGGCNRV 159
                          90
                  ....*....|....*
Feature 1                        
gi 79521972   150 LKVPNSGNFSVVCHW 164
gi 157353332   86 LKLPKGGSFGVVCLW 100
gi 125579082  114 VELPKDGKFDIVCHW 128
gi 162696562   89 ISLPDEGDFELVTHW 103
gi 116792294  160 LELPKEGNFEIVCHW 174

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