1QHO


Conserved Protein Domain Family
CBM20_novamyl

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cd05820: CBM20_novamyl 
Click on image for an interactive view with Cn3D
Novamyl (also known as acarviose transferase, ATase, maltogenic alpha-amylase, glucan 1,4-alpha-maltohydrolase, and AcbD), C-terminal CBM20 (carbohydrate-binding module, family 20) domain. Novamyl has a five-domain structure similar to that of cyclodextrin glucanotransferase (CGTase). Novamyl has a substrate-binding surface with an open groove which can accommodate both cyclodextrins and linear substrates. The CBM20 domain is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch.
Statistics
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PSSM-Id: 99893
View PSSM: cd05820
Aligned: 4 rows
Threshold Bit Score: 167.391
Threshold Setting Gi: 89241792
Created: 22-Jan-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
starch bindingstarch binding
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:starch binding site 1 [chemical binding site]
Evidence:
  • Comment:Site 1 is small relative to site 2 and exhibits minimal structural changes upon ligand binding. It acts as an initial starch recognition site.
  • Structure:1QHO_A; Geobacillus stearothermophilus maltogenic alpha-amylase binds maltose; defined at4A contacts.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                            #                                      #    
1QHO_A       580 TQTSVVFTVKsa----ppTNLGDKIYLTGNIPELGNWSTDtsgavnNAQGPLLA-PNYPDWFYVFSVPAGKTIQFKFFIK 654
gi 119720359 633 RQIPVIFEVQntkgttleTVPGEFLWLTGSVPELSNWSPAt----tRAVGPMLC-PAWPNWFVVASVPANTYIEFKFLKA 707
gi 6552352   633 KQIPAIFEVRntqgtnleTQVGEFLWLTGSVPELSYWSPEt----iKAVGPMLC-PGWPDWFVVASVPADTYIEFKFLKA 707
gi 89241792  621 KPVPVQFTVQnp----paTAPGESLYLTGDVAELGHWSTSp----dQTAGQLLRvPNESRGVLVADLPAGAPVEFKFVKV 692
                         90       100       110
                 ....*....|....*....|....*....|..
Feature 1               ##   #                   
1QHO_A       655 RaDGTIQWENGSNHVATTPTGATGNITVTWQN 686
gi 119720359 708 PlGGTGVWEPGSNHAYTTPSDGIGRVSVTANG 739
gi 6552352   708 PlGGTGIWEVGSNHAYLTPSSGIGEVSVEANR 739
gi 89241792  693 AaDGTVTWEGGANHRYTVPAGGTGTTTLTWQR 724

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