Conserved Protein Domain Family
Sortase_E

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cd05830: Sortase_E 
Sortase domain found in the class E family of sortases
Class E sortases are membrane-bound cysteine transpeptidases distributed in Gram-positive bacteria (mainly present in Actinobacteria). Genes encoding class A and E sortases are never found in the same organism, and similar to class A sortases, the genes encoding class E sortases are not positioned adjacent to genes encoding potential protein substrates, suggesting a housekeeping sortase function of class E sortases in some high G + C Gram-positive bacteria. Similar to class A sortase, class E sortases are capable of anchoring a large number of functionally distinct surface proteins containing a cell wall sorting signal to an amino group located on the bacterial cell wall. They recognize an LAXTG sorting signal, instead of the canonical LPXTG motif processed by class A sortases. The prototypical class E sortase from Corynebacterium diphtheria (named Cd-SrtF) is a non-polymerization sortase that is not required for pilus polymerization, and proceeds to complete the assembly process by anchoring the polymer to the cell wall peptidoglycan. Moreover, in Streptomyces coelicolor, one or both of Staphylococcus aureus SrtA homologs may function as class E sortase responsible for the cell wall anchoring of the long chaplin proteins (ChpA-C) containing an LAXTG sorting signal, which presumably mediate aerial hyphae formation. The family also includes some class E sortase homologs from Gram-negative and Archaebacterial species, but the functions of these enzymes are unknown.
Statistics
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PSSM-Id: 320679
View PSSM: cd05830
Aligned: 196 rows
Threshold Bit Score: 78.0183
Threshold Setting Gi: 950257964
Created: 29-Oct-2007
Updated: 18-Aug-2016
Structure
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Aligned Rows:
 
putative
Feature 1:putative catalytic site [active site]
Evidence:
  • Comment:The catalytic triad is formed by His, Cys, and Arg.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                 #                      
gi 81718551   87 KDGIGFLHVPamnNGEVLVRKGtstqVLNNGVAGYYTdpvkamlpmtgknGNFTLAAHRDghgAKFHNIDKVRkGDPVVF 166
gi 499279662  81 DWEDVRLVMPt-lGEDVRVVEGvdlgALSQGVGHWPGtaep------gqtGNMVIAGHRTthgAPFRRLDELRvGDDVGI 153
gi 684293395  86 EIIIGEIVIPa-lMVSQTIYQGmslpTLDKGVGYWPGtamp------gyvGNVVLGGHRVskkKPFRNIDQLVaGDEIYL 158
gi 684285253  18 YKRVGTLSISk-lKVTSPIYMGitddVFNRGIGQWPGsakp------gtrGNLVLGGHRTsaqREFADIDRLRgGDIISI 90
gi 229567893 124 YEAMGRIQIPa-iGLDAEYGNGvhesALARGPGHWPGtpap------gqaGNAVISGHRTthtAPFRDLDDLVeGDEIVV 196
gi 919207506  87 YVPMGTIQIPa-iGLDVEYGSGvheaALVHGPGHWPGtpap------gqpGNPVISGHRNthtQPFKKLDQVEvGDVIIA 159
gi 502685967  37 QLALGRLAIPr-lKLDDELNNGvdeaALVRGVGHWPGtplp------gspGNAVISGHRStneKPFLHLDKLRpGDPIKV 109
gi 916404077 121 GERIGTVSLPr-gGKSSAIREGvsaeVLERAVGHYPGtqlp------grpGNAVLFGHRTmgaAPFADLDKLRrGDVVDV 193
gi 893638912  52 GARLGQIRIKr-mHLKADVRQGvgqaVLRRAVGHYPRtgvp------grpGNTVLLGHRTtwlHPFNKLDRMRrGDRIVL 124
gi 517483802  82 GTELGALDITp-lRVRGTIREGvgndILDRGVGHYPGtemp------gelGNTVLLGHRTtgpKPFLNLDRLRpGDLIGV 154
                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
Feature 1                                                       #        #      
gi 81718551  167 ET-KDDWYVYKVFAILPEtskynvkvvaglpkqagvtkpGRYITLTTCTPVytSEYRYIVWGK 228
gi 499279662 154 GA-SGQVVTYRVTGTEIVdpgqve---------varpttTATATLFACHPPgsAAKRIVVRLA 206
gi 684293395 159 TT-SDGTFVYSVTEQLVVeptetw---------iinqtdTATLTLFACHPPhsTKQRLVVFAD 211
gi 684285253  91 SL-GTKTHKYVVTGHMIVkptsvw---------itdqtsSATLTLFSCHPKgkITSRYVVRAV 143
gi 229567893 197 ST-GAAEPVTYVVVDTVVvpvesyteh---vlappddatSRELTLFACHPEgsLANRIVVHAR 255
gi 919207506 160 SAyGGPATEYTVFNTVIVpeaeyedyv----lqqpddptVREITVFACHPEgnPVNRIIVQAR 218
gi 502685967 110 TV-GTRSTTYRVVRTTIVaesayvpfv----lrkpskpsDRVITLFACNPLtaHYQRIVVEAR 167
gi 916404077 194 RS-GGARYRYRVRTSRVVtpnrtdvlna--tpfgsdrrsGSYLTLITCTPKgsNLRRLVVVAT 253
gi 893638912 125 RW-GGTTYVYRVRTTRVIdprdrralep-vpfkrrsaphGQYVTLITCTPKgsDRHRLVVVGK 185
gi 517483802 155 TA-KKHRYSYRVRELRVIepdetdvlap-tpfrkrkgktGTFLTLITCHPKgeDTRRLVVVAT 215

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