Conserved Protein Domain Family
H2MP_Cyano-H2up

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cd06063: H2MP_Cyano-H2up 
This group of endopeptidases include HupW enzymes that are specific to the cyanobacterial hydrogenase and are involved in the C-terminal cleavage of the hydrogenase large subunit precursor protein. Cyanobacterial nickel-iron (NiFe)-hydrogenases are found exclusively in the N2-fixing strains and are encoded by hup (hydrogen uptake) genes. These uptake hydrogenases are heterodimers with a large (hupL) and small subunit (hupS) and catalyze the consumption of the H2 produced during N2 fixation. Sequence similarity shows that the putative metal-binding resides are well conserved in this group of hydrogen maturation proteases. This group also includes such proteins as the hydrogenase III from Aquifex aeolicus.
Statistics
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PSSM-Id: 99874
View PSSM: cd06063
Aligned: 7 rows
Threshold Bit Score: 226.507
Threshold Setting Gi: 98152884
Created: 21-Dec-2007
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
nickel binding
Feature 1:nickel binding site [ion binding site]
Evidence:
  • Comment:based on sequence similarity to HybD structure, 1CFZ_A
  • Comment:Replacement of the homologous metal binding residues in HycI (hydrogenase 3 processing endopeptidase) by site-directed mutagenesis suggest a role in catalysis.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                     #                                               #                  
gi 72537510    2 LTIIGCGNLNRCDDAVGVIIAQRLQqyliqnphpnVRIFDCGTAGMEVMFQARGSkQLIILDASsTGSePGAVFEVPgSE 81
gi 98152884    9 TAIIGCGNINRGDDGAGPAVIARLAaedl---psdVELLDAGTDGMAVMYRARGAqQLIVIDARePEGkPGAIYEVPgDV 85
gi 17228918    2 LTIIGCGNLNRSDDAVGVIIAQRLQkylaenphphVQVYDCGTAGMEVMFQARGSkQLVIIDASsTGSePGAVFKVPgEE 81
gi 86739786   33 VAVIGCGNLLRGDDGVGPILVRRLAetgv---pagVRLVDGGTAGMDTAFAMRGArRVVVVDASrTGAePGTVFEVPgAQ 109
gi 152061253   4 VVIVGCGNPIRGDDGVGPRLIRYLWergl---pphIKLVDGGTSGIDVVFHMEGAdEVVFVDACcTGEkPGTVFCVPhEE 80
gi 161075695   3 TTIIGFGNMLRKDDGLGPELIRRLMalnp---plpVKLIDYGTAGFSFLNELETEnRLIIIDAVsSGSaPGSLFVLEkER 79
gi 45643436    2 LTIIGCGNINRSDDAVGVVVVQRFQqflkehpcsdVRIYDCGTAGMEVMFRARGSkELIIVDACmTGSePGMIFKIPgSV 81
                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
Feature 1                      #                                                         
gi 72537510   82 LEQLPEPSy---NLHDFRWDHALAAGRRIFqEDFPqAVSVYLIEANSLefGWELSSPVEQAAEKVFQSIIQI 150
gi 98152884   86 LAAEPVHGv---GLHAFRWDNALFVGRKIFgDDFPsDVQVYLIEAQTLdyATELSGPVVAAVEKVAAKIAAR 154
gi 17228918   82 LAALPEPSy---NLHDFRWDHALAAGRKIFpDDFPqDVTVYLIEAANLdfGLELSPVVQQSADLVVEKIVEI 150
gi 86739786  110 LENLPLDAp---HPHAFRWDQALAFGRWLLgDGYPdDVVVYLIEAGGVepGADLSPAVRRAMEQVRDMIISG 178
gi 152061253  81 VEELPSSEe--aNLHSIKWYHAVAIARHLLnGNYPkSIKVYLIEGKSFgiGEVLSEEVESAMKRLAERIIKE 150
gi 161075695  80 LELVLKEReeypNLHTFRWNDALRWGRWFLkGNFPrNVRVYLVEVKETgyGIGLSEPVKEAVERLVALFKKE 151
gi 45643436   82 LEVLPEQSy---SLHDFRWDHTLAAGRKIFrNDFPkEVTVYLIEVESLdfGLELSPSVKDAAEQVLEMLQDQ 150

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