Conserved Protein Domain Family
H2MP_F420-Reduc

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cd06064: H2MP_F420-Reduc 
Endopeptidases belonging to F420-reducing hydrogenases group. These hydrogenases from methanogens are encoded by the fru, frc, or frh genes. Sequence comparison indicates that fruD and frcD gene products from Methanococcus voltae are similar to HycI protease of Escherichia coli and are putatively involved in the C-terminal processing of large subunits (FruA and FrcA respectively). FrhD (F420 reducing hydrogenase delta subunit) enzyme belongs to the gene cluster of 8-hydroxy-5-deazaflavin (F420) reducing hydrogenase (FRH) from the thermophilic methanogen Methanobacterium thermoautotrophicum delta H. FrhD subunit is putatively involved in the processing of the coenzyme F420 hydrogenase-processing. It is similar to those frhD genes found in Methanomicrobia and Methanobacteria. It is different from the FrhD conserved domain found in methyl viologen-reducing hydrogenase and F420-non-reducing hydrogenase iron-sulfur subunit D.
Statistics
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PSSM-Id: 99875
View PSSM: cd06064
Aligned: 20 rows
Threshold Bit Score: 186.293
Threshold Setting Gi: 124484843
Created: 10-Dec-2007
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
nickel binding
Feature 1:nickel binding site [ion binding site]
Evidence:
  • Comment:based on sequence similarity to HybD structure, 1CFZ_A
  • Comment:Replacement of the homologous metal binding residues in HycI (hydrogenase 3 processing endopeptidase) by site-directed mutagenesis suggest a role in catalysis.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                    #                                                       #           
gi 44702      10 LVLGCGNILFGDDGFGYHMIKRLNelkeqypilnsekIQLIDAGTGAFSLYFIINRWrt-plKKIVIADIIDYGl-ePGT 87
gi 20095060   30 LIVGCGNELFGDDGFGPAVIKEIErrgw-----ehpdVEILDAGAGAPQNVFSLIDEds-kvEYMVVVDAVDVGa-ePGT 102
gi 88603576    7 VICGCGNPLFADDGFGPAVVEELQkldl------penVKVIDAGLGGPHFLFTLLAQsdepvKHLFIIDIADFGg-kPGE 79
gi 51244860    9 MIFGCGNITMGDDGFGPAVIDELHahye-----lpegVQAIDADTGIREYLFDYLLSeedrpDRIIVLDAVDFPdrnPGE 83
gi 116750948  11 LIFGCGNVLLGDDGFGPAVVEYLLehft-----lpddVYAVDVGTGIRDLLIDYVLMpd-kpKRIFIVDSICRPmrkPGE 84
gi 157404497  13 VVFGCGNILYGDDGFGPAVADYLNahsr-----vpsfACVMDVGTAIRELLFNIIISdv-kpKVIVLVDAMETGl-eVGE 85
gi 161404643  12 LVLGCGNPLFGDDGFGPAVIEALEnass-----lpegCVVLDAGTSVREILFDICVSpe-apDRVIVVDAHHEPgcePGR 85
gi 124484843   9 IIAGVGNELFGDDGFGPVVIRALAdyel------pdnVKAMDVGLGGPHLIFSMLDPev--tKKLIIVDAMDFGg-kPGD 79
gi 20089853    9 VVAGCGTPLYADDGFGPAVVESLKglel------penVTVVDAGLGGPHFVFTLLNPes--tKTLIIVDIADFGg-ePGE 79
gi 154150021   7 VIVGCGNPLFGDDGFGPAVIEEMEklil------pdnVTIQDGGAGAPHFIFNFLDPev--tKKLVVVDISDFNa-kPGS 77
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                            #                                                           
gi 44702      88 LKKLCvEDIPNLPky-hvDAHDMPLAGMLREInddygIEIVVIGCQQKRVtapd---illELSEEVTNSIDDAVKMVVDE 163
gi 20095060  103 LLEFGpEDLDPNCrivpvDAHGWSIESALLDLnerigIDFRILGCQVKELpipe---vqpGLSDPVRKAVPKAADRAIEL 179
gi 88603576   80 IAIISpDDLPPGKy---rDAHSWDLTEPIHQLkn--rVKIDIIGCQPKRVsepe---feiGLSEEVTEAIPRTVQLVLKM 151
gi 51244860   84 VFYIApDSIPSQKih-dfSLHQFPTVNMLLELeqytgMEIKILAAQIECIpke----iapGLSEPMSKAVPAACEMISQI 158
gi 116750948  85 LLEVDlSELGVQKqh-dfAVHQFPSTNLLEELrseggVEVRVLAVQVKALpee----vrpGISDEVAAAVPRACEWLLEQ 159
gi 157404497  86 LGHLTvEQVSPEKin-dfSLHLMPTLNMLKELhadggVKIDVLAVKPERIpee----meqGLTEKVQKAVVETARYITDT 160
gi 161404643  86 IREIGvEGLAHEKls-dfSMHQFPTMNLLAEFadktgKEVRVFVAQTCGIpdr----mapGMSPAVEAAVGRMVRFLLKL 160
gi 124484843  80 VTKIPaDLLPEGRgerymDAHSGNLLDPIGRLkg--kMDIVIIGCQPGYIpapdsedfalELSPEVQGAVPKTVTVILQE 157
gi 20089853   80 LRWLAvDELPVGSy---rDAHSWDLAEPLQYIkd--dIRIRVLACQKKYVstpe---mviGITDEVQRAIPDAVSEILKE 151
gi 154150021  78 ISKISgDTLKPGAy---iDPHSWDGVDQLCRIsa--kIDTTVVMCQPAKAikkgdadveiGLSEDVKNAIPKAIQVILET 152

                 .
Feature 1         
gi 44702     164 L 164
gi 20095060  180 A 180
gi 88603576  152 I 152
gi 51244860  159 L 159
gi 116750948 160 V 160
gi 157404497 161 Y 161
gi 161404643 161 I 161
gi 124484843 158 L 158
gi 20089853  152 I 152
gi 154150021 153 I 153

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