Conserved Protein Domain Family
H2MP_NAD-link-bidir

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cd06066: H2MP_NAD-link-bidir 
Endopeptidases that belong to the bidirectional NAD-linked hydrogenase group. This group of endopeptidases are highly specific carboxyl-terminal protease (HoxW protease) which releases a 24-amino-acid peptide from HoxH prior to progression of subunit assembly. These bidirectional hydrogenases are heteropentamers encoded by the hox (hydrogen oxidation) genes, in which complex HoxEFU shows the diaphorase activity, and HoxYH constitutes the NiFe-hydrogenase.
Statistics
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PSSM-Id: 99876
View PSSM: cd06066
Aligned: 28 rows
Threshold Bit Score: 139.269
Threshold Setting Gi: 118047433
Created: 10-Jan-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
nickel binding
Feature 1:nickel binding site [ion binding site]
Evidence:
  • Comment:based on sequence similarity to HybD structure, 1CFZ_A
  • Comment:Replacement of the homologous metal binding residues in HycI (hydrogenase 3 processing endopeptidase) by site-directed mutagenesis suggest a role in catalysis.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                    #                                                #                  
gi 89902613   12 LVFGWGNLSRGDDALGPLCIERLRalaa---pataVDYLDDYQlQIEHALDLVgrqRVLFVDASlsc-paPFELTQLQAA 87
gi 53802645    5 LVFGYGNPSRGDDALGPLFLEALQirlas-spgvdIEFLQDFQlQIEHALDLAgrdRVLFVDAHvgc-ppPFRFERLVPQ 82
gi 82702799   25 LVFGYGNPSRGDDALGPLLIERLEald-----lpnVELLTDFQlQIEHALDLQsreRVLFVDTSisc-vpPYAFSRLLAR 98
gi 5020381    11 VVFAWGNESRGDDALGPPAGPGIAlag-----wshITLIEDVQlQVEHALDLVgqdLALFYDAGmgt-paPFAFVQAVPR 84
gi 71906622    6 VVFAVGNPSRGDDAIGPELCGRLEkwldheglgerVELIEDFQlQIEHALDLQgrqLALFIDAGant-paPYTFQRIVPA 84
gi 119944965  10 NLFTWGNPSRGDDAIGPVLHEFIKqficr-fdlghIQLVEDFQlQPEHVCDITdnaCVVFIDASyqg-esPFQIQPVSAK 87
gi 119897707   6 VVIAVGNDARGDDALGPLLLDRVVkag-----fpgVRAQLEFQlQVEHALDISgagLVLFIDAAhgl-htPYELSELEPA 79
gi 1617574    21 LIYGIGNVGRQDDGLGWAFIDWIEeegl----cpkAEILRHYQlHLEDAELIShkkRVLFVDATkdasvtSFALERAEPR 96
gi 38637757   21 LIYGIGNVGRQDDGLGWAFIDRLEaesl----csgAEVQRHYQlHLEDADLISrkrKVLFIDATkdasvaSFSLERAEPR 96
gi 83643015   11 LVIGYGNPGRQDDGLGPACADRIAeln-----lsgVTVETNYQlTVEDALELSryqQVVFIDASlrg-gkPFFLQQIEAD 84
                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
Feature 1                #                                                          
gi 89902613   88 RDISFTT-HALSPQALMQVFFDLrgeapPSCTLLAIRGECFELGa-pPSPAALAHLAAALAWATDWL 152
gi 53802645   83 RDGSYTT-HAVSPQALLEVYRRInrdepPPAYLLSIRGERFDLGd-aLSRSAESNLESALRFAEVLI 147
gi 82702799   99 KDVSFTS-HVMSPIALLYAYLNLyg-ppPPAYLIEIRGDCFELGe-pLGPRAAANLDASFQLARRLC 162
gi 5020381    85 RFLGPSTsLGIGPEGVLEAYRQVmhdepPPAFVLCLRGESFALEe-gLSAAARAHLAQAWGFLIPLL 150
gi 71906622   85 TGNAYTT-HELPPEAVLQVYVQTenaepPPAFVLCVRGEQFELGe-gFCVAAALHLDAAFDLLTRLL 149
gi 119944965  88 NELGYTT-HALSPAALMAIYAQTqnkpcPPAFLFSVRGYSFELGa-pLSKQAQENITLATQFLKKLL 152
gi 119897707  80 RATPAFS-HALAPAAVLAVLEQLeg-haPPAFLLRVRGEQFGLGd-sLSPLAEAALPLAWELLATLL 143
gi 1617574    97 MDFSFTS-HAISIPAIMATCQQCfq-rlPDVYVLPIKGYEWELEl-gLTQRARQNLNAATARLSIRT 160
gi 38637757   97 MDFSFTS-HAISIPSIMATCQRCfq-clPEVYVLAIRGYEWELRm-gLTPQARHNLDDAIAHFSMRA 160
gi 83643015   85 DERQMGS-HVLSPQAVVTLSRTLfd-anTQAFLMGIRGYAFDRFeeaLSPEAQLNLNQAFDYLASWL 149

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