Conserved Protein Domain Family
H2MP_MemB-H2evol

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cd06067: H2MP_MemB-H2evol 
Click on image for an interactive view with Cn3D
Endopeptidases belonging to membrane-bound hydrogen evolving hydrogenase group. In hydrogenase 3 from E coli, the maturation of the large subunit (HycE) requires the cleavage of a C-terminal peptide by the endopeptidase HycI, before the final formation of the [NiFe] metallocenter. HycI protease is a monomer and lacks characteristic signature motifs of serine, zinc, cysteine, or acid proteases and thus its cleavage reaction is not inhibited by conventional inhibitors of serine and metalloproteases. Such hydrogenases as those from Methanosarcina barkeri (EchCE) and Rhodospirillum rubrum (CooLH) also belong to this group of membrane-bound hydrogen evolving hydrogenase. Sequence comparison of the large subunits from related hydrogenase indicates that in contrast to EchE (358 amino acids) and CooH (361 amino acids), the large subunit HycE (569 amino acids) contains an extra carboxy-terminal stretch of 32 amino acids that is cleaved during the maturation process. In the absence of this C-terminal stretch, there is no homolog of endopeptidase HycI found in these two related hydrogenase.
Statistics
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PSSM-Id: 99877
View PSSM: cd06067
Aligned: 30 rows
Threshold Bit Score: 127.668
Threshold Setting Gi: 149194126
Created: 10-Dec-2007
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
nickel binding
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:nickel binding site [ion binding site]
Evidence:
  • Comment:based on sequence similarity to HybD structure, 1CFZ_A
  • Comment:Replacement of the metal binding residues in HycI by site-directed mutagenesis suggest a role in catalysis.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                    #                                                #                  
2I8L_A         4 VLLCVGNSMMGDDGAGPLLAEKCAaap---kgnWVVIDGGSAPENDIVAIRelrPTRLLIVDATdmg-lnPGEIRIIDp- 78
gi 34558133    4 VLLCVGNELRGDDGVAIALGRLVEeqm----peWSVFFGYDTPESEFGKLRelaPDVIVVADAMsg--fkEGEIEFLDl- 76
gi 118474305  11 SLLCIGNKMRGDDAVGLYVGELVEeqm----psWRVFYGQDVPENEFGAIRefaPDIIVVVDAMsg--fkDDKIEFFNl- 83
gi 149194126   4 ALLVVGNPLKGDDSVAIKLGEMIEkl-----egWRVFFGNDTPEDEVFKLKkysPDLVVVADAVig---iEGAEFLSL-- 73
gi 3257393     4 VICCIGNELKGDDAFGILVYEMLKgki---rdeALLINCGNVPENYIGKIIdadPDLVILVDAVhfg-gkPGELVIVDpe 79
gi 18976989   15 VICGIGNDIRGDDSFGVYIAEKLKrvik--kanILVLNCGEVPENYTGKILnfhPDLIIFIDAVnfg-gkPGEIIITDp- 90
gi 57641939   15 VICGIGNDVRGDDAFGVLVAERLKelvk--tpdVLILNCGEMPESYVGKIAafkPDLVVFVDAIhfg-geIGEFIIADpl 91
gi 126466341  27 VIACVGSPLRSDDRLGLLIYDKIKdla---nnnISIIECEYGLENCFTEIVaenPATLLIIDAVyneslnPGTTVLTSi- 102
gi 118581203  16 CFMGVGSTDYGDDGFGIRLAEKLEqnlakranaPVVIDAGTMPERYTGRIAdegYDSLVFLDAVdfg-gePGAVLVANs- 93
gi 83591662   16 LVVGAGNPLRGDDGAGPRLVEMLTgk-----vnAALLDVGEIPEAYTRRILetrADTILFVDAAdfg-aqPGDFALLEe- 88
                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
Feature 1                    #                                                      
2I8L_A        79 DDIAEMf-mMTTHNMPLNYLIDQLKed--igEVIFLGIQPDIVGFyy---pMTQPIKDAVETVYQRL 139
gi 34558133   77 SDERTYl--YSTHNLPTPILISYLRgi--csKTIFLGISVLLENVlhfsegLSQGASDSAFVALGRI 139
gi 118474305  84 ENERDYi--YSTHNLPTPVLLSYLRti--tpKTLFLGISVLLENVlgfeegLSQNAKISAKKAVQKI 146
gi 149194126  74 QKDANYl--YSTHNIPLYLIIEFIKef--cdNVLFLGIGVDEKNLgkitfeISNKALNSLNEAFNKL 136
gi 3257393    80 ETLGEA---FSTHSLPLKVLTKFIRent-raKVLLLGCQPRNVEFlg---vVSEEVKAAAERATNYI 139
gi 18976989   91 ENTEGAg--VSTHSLPLKFLATYLKant-naKTILIGCQPKNIGLfe---dMSEEVKAVAEVLLKFL 151
gi 57641939   92 KTLGEA---VSTHGLPLRIVASYIKeqt-gsDIVLIGCQPGSTGLfe---ePSELIKERAERLAELI 151
gi 126466341 103 ENIREKislATTHTIPLRMILELIMkktnikNTYILGIRAKNLDIgm---hVSPEIKKVVEDFAKIL 166
gi 118581203  94 EEMASRfpqISTHKISLGMLANWIEasg-ttKAWLIGVQPESLKMgq---gVSPTVQDTLDILEELL 156
gi 83591662   89 ETIAAGh--AGTHQLPLTLFFQYLResg-ggEIVVLGIQPQSISLgt---pMSAAVSRTLASLSDLL 149

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