Conserved Protein Domain Family
H2MP_like-1

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cd06068: H2MP_like-1 
Putative [NiFe] hydrogenase-specific C-terminal protease. Sequence comparison shows similarity to hydrogenase specific C-terminal endopeptidases, also called Hydrogen Maturation Proteases (H2MP). Maturation of [FeNi] hydrogenases includes formation of the nickel metallocenter, proteolytic processing and assembly with other subunits. Hydrogenase maturation endopeptidases are responsible for the proteolytic processing, liberating a short C-terminal peptide by cleaving after a His or an Arg residue, e.g., HycI (E. coli) is involved in processing of HypE (the large subunit of hydrogenases 3). This cleavage is nickel dependent.
Statistics
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PSSM-Id: 99878
View PSSM: cd06068
Aligned: 10 rows
Threshold Bit Score: 167.512
Threshold Setting Gi: 38637735
Created: 27-Dec-2007
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
nickel binding
Feature 1:nickel binding site [ion binding site]
Evidence:
  • Comment:based on sequence similarity to HybD structure, 1CFZ_A
  • Comment:Replacement of the homologous metal binding residues in HycI (hydrogenase 3 processing endopeptidase) by site-directed mutagenesis suggest a role in catalysis.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                    #                                                     #             
gi 134099601   6 LVAGIGNVFLGDDGFGVEVVGRLLeeeh-------pagVRVADFGVRGVHLAYELMNG-YDAVVLVDAVPlseePGTVQV 77
gi 88804049    9 LVAGVGNELKQDDAFGIEFAKKFRdavs------hldhIMVMEVGIGGIHLVQELHAG-YDILILADAVAwgeeAGHIYF 81
gi 111021581   8 LVAGVGNIFFGDDGFGPEVLRGVAtrtl-------ppgVRAVDFGIRGTHLAYELLDG-WGALLLVDAVPgrgePGALRA 79
gi 116625612   6 LVAGVGNIFLGDDAFGSEVARRMLnekw-------pedVRVVDFGIRGFDLTFALLEG-YDTVILVDATPrggaPGTLYT 77
gi 111221820  14 LVAGVGNIFLGDDGFGVEVVRRLDpanl-------pgeVDVADYGIRGLHLAFALLDGrYDTVILVDALPsdepPGTLTV 86
gi 120403400  13 LVAGIGNIFLGDDGFGPEVIRRIPhrla-------gpcVRLKDYGIRGMHLAYDLLGP-WSALVLVDAVPdrgaPGTVHM 84
gi 38637735    5 LVAGIGNVFLGDDGFGVEVVQRLGarqaahalppfpegVVVADFGIRGIDLCYALLDG-VDAAILVDATQrggaPGSLYV 83
gi 29833914   12 LVAGIGNIFLGDDGFGVETARRLAerql-------pahIEVVDIGVRGVHLAYQLLDG-YDTLILVDATArgeaPGTLYL 83
gi 158314317   4 LVAGVGNILLGDDGFGVQVARHLRrtgp-------pprVTIADYGTSGLHLAFDLCSGgYSTVVLVDALGrgyePGTVSL 76
gi 126434711   2 LIAGIGNIFLGDDGFGPEVLRRAAplit-------dprVRAVDYGIRGMHLAYDLLEP-WDLLVLVDALPdrgsPGTLHV 73
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                          #                                             
gi 134099601  78 VE-----------VDLDAVepaapg-aaapvmDAHGMTPDAVFGLFRALGGsv-----gRALVVGCRPLDCse------r 134
gi 88804049   82 REm----------EKVSDIeempvfekrtflaDMHYTNPIRALMLAKALKVlp-----nEVYILGCEAAQHddf-----a 141
gi 111021581  80 FEvg--------pDSVPAAp-----------lDAHSMDPGAVFGSLRALGGtl-----pRTVVLGCQVANTge------g 129
gi 116625612  78 IE-----------PELDDGeag--------evATHGMNPMRVLAMAKHMGAew-----kRLLVVGCEPAHDavnd---gg 130
gi 111221820  87 LR-----------PEVGDAptgda----aavvDAHGMSPDVVLRLVRDLGGei-----gQVVVVGCRPAVVad------r 140
gi 120403400  85 FEad--------pETLTTPta----------lDAHAMDPGAVFACLNALGGaa-----pRTVVVGCEVETVee------g 135
gi 38637735   84 IElpmdaqdgdgaGDPYASpv---------lmSPHEMNPALVLRALRMMGGtc-----aDVVVIGCEPADFglgegeegr 149
gi 29833914   84 IEhds-----pggGEPEGApv----------lDGHRMTPDTVLALLDTLCAgtggqpprRTLIVGCEPASVee------g 142
gi 158314317  77 VDl----------TDVRAPse----------vDAHGMRPDVVLDLVDTLGGhl-----dRVLLVGCEPASVdh------q 125
gi 126434711  74 FEad--------hDALCATgg----------lDAHAMDPAAVFASLTALGGap-----pRTVVIGCEAADVte------g 124
                        170       180
                 ....*....|....*....|..
Feature 1                              
gi 134099601 135 mGLSEPVAGAVDRAAGLVRELV 156
gi 88804049  142 vGMSEAVEDALPKAMRKLQNWL 163
gi 111021581 130 iGLSPRVEAAVGQAVDLVFDIV 151
gi 116625612 131 iGLSEAVRPAVAEAVEVVRRLI 152
gi 111221820 141 mELSGAVRAAVDGAVDLIGDIL 162
gi 120403400 136 iGLSETVAAAVPAAVRAVEDVV 157
gi 38637735  150 lGLSEPVAAAVDRAVAMVEAMV 171
gi 29833914  143 iGLSPPVSDAVPEAVRLIDELL 164
gi 158314317 126 vGLSPPVAAAVEPAARLVRSVI 147
gi 126434711 125 iGLSPPVAAAVPEAVRAVGAVV 146

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