Conserved Protein Domain Family
H2MP_like-2

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cd06070: H2MP_like-2 
Putative [NiFe] hydrogenase-specific C-terminal protease. Sequence comparison shows similarity to hydrogenase specific C-terminal endopeptidases, also called Hydrogen Maturation Proteases (H2MP). Maturation of [FeNi] hydrogenases includes formation of the nickel metallocenter, proteolytic processing and assembly with other subunits. Hydrogenase maturation endopeptidases are responsible for the proteolytic processing, liberating a short C-terminal peptide by cleaving after a His or an Arg residue, e.g., HycI (E. coli) is involved in processing of HypE (the large subunit of hydrogenases 3). This cleavage is nickel dependent.
Statistics
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PSSM-Id: 99879
View PSSM: cd06070
Aligned: 6 rows
Threshold Bit Score: 154.125
Threshold Setting Gi: 156937298
Created: 10-Jan-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
nickel binding
Feature 1:nickel binding site [ion binding site]
Evidence:
  • Comment:based on sequence similarity to HybD structure, 1CFZ_A
  • Comment:Replacement of the homologous metal binding residues in HycI (hydrogenase 3 processing endopeptidase) by site-directed mutagenesis suggest a role in catalysis.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                    #                                           #                       
gi 119871714   3 LVVGLGNVVYGDDGFGSCLAQVLSh---yNDFVFDGNAhGIGVLGTLadyDILVFLDIDvr-----lpPGAVAIERv--- 71
gi 156937298   5 LVLGLGNRNLGDDAVGSCLAEALAarapeGVEVKDGDDmGLGLLGFLegyDLVIFVDASpdv----edVEAFLIEAe--- 77
gi 119719502   8 LIMGVGNRMYGDDAFGPLFAEALEq---cGVPALNSELdVFTAVSHLegvDLLIFIDVVdksfgepgdVVVLRIDPrtls 84
gi 146303696   4 LIAGVGNIFMGDDGCGSIVAEALKgc-vsGAEVIDIGTgGLPLTDYLenfDVVILVDAAvid----edVKVIEVTDem-n 77
gi 22204184    4 KIIGLGNRLYGDDAVGSLTAACMEe---lGLPAFDAGAnGFQALSAIengDIVFFIDIVqmd----eeEGIFKVDLd--- 73
gi 15898065    3 AIIGVGNRLMKDDGFGSCLAEVIInk-vkNAVVVDLGL-GNLLSVDLdkyDTVIIIDVAnin----eeYGIFKITSt--- 73
                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
Feature 1                        #                                                        
gi 119871714  72 eGSLTLQetr--liDAHRTPPSLLVGYLramg-rdpKAYIIAVGPKTLepfsPPSQEVIKAVPIAVEELKKLL 141
gi 156937298  78 kAELEEArem--vqDSHKAGPVALAVLAkksglfqgKAYFVAFKPESLcfmcKPSEASLERALKAAELVNKLL 148
gi 119719502  85 pEEVATAisf--etSAHQVTPAHIVLLGyssgffrgEAYVVGVVSEHTdfarKMSSRVVAAAERACLKLREIA 155
gi 146303696  78 pDSVGEGvlsllfgGSHGLGVMDLLNFLkire-rkpKVLIVGCRPISVevrmGLSEGMTANCLKALDEIARLG 149
gi 22204184   74 kADFVEIt------DPHRLTPLQVLSLSarsnnrpkEAYIVGIKPEYIdw-pGISNAAIKRLEKVLQKFKKFI 139
gi 15898065   74 sKGILEQ-------SLHELGLNTILKLYed-----kQFYIIVCKPEEIqigyGLSKDCLLRIEKLIPEFKAFL 134

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