1PPL,3APR


Conserved Protein Domain Family
Aspergillopepsin_like

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cd06097: Aspergillopepsin_like 
Click on image for an interactive view with Cn3D
Aspergillopepsin_like, aspartic proteases of fungal origin.
The members of this family are aspartic proteases of fungal origin, including aspergillopepsin, rhizopuspepsin, endothiapepsin, and rodosporapepsin. The various fungal species in this family may be the most economically important genus of fungi. They may serve as virulence factors or as industrial aids. For example, Aspergillopepsin from A. fumigatus is involved in invasive aspergillosis owing to its elastolytic activity and Aspergillopepsins from the mold A. saitoi are used in fermentation industry. Aspartic proteinases are a group of proteolytic enzymes in which the scissile peptide bond is attacked by a nucleophilic water molecule activated by two aspartic residues in a DT(S)G motif at the active site. They have a similar fold composed of two beta-barrel domains. Between the N-terminal and C-terminal domains, each of which contributes one catalytic aspartic residue, there is an extended active-site cleft capable of interacting with multiple residues of a substrate. Although members of the aspartic protease family of enzymes have very similar three-dimensional structures and catalytic mechanisms, each has unique substrate specificity. The members of this family has an optimal acidic pH (5.5) and cleaves protein substrates with similar specificity to that of porcine pepsin A, preferring hydrophobic residues at P1 and P1' in the cleave site. This family of aspartate proteases is classified by MEROPS as the peptidase family A1 (pepsin A, clan AA).
Statistics
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PSSM-Id: 133161
View PSSM: cd06097
Aligned: 23 rows
Threshold Bit Score: 249.91
Threshold Setting Gi: 34500105
Created: 18-Jan-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic motif [active site]
Evidence:
  • Comment:Catalytic motif in both N- and C-terminal lobes of this family.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                          ####                                                          
1PPL_E        17 YITPVTIGg--tTLNLNFDTGSADLWVFstelpasqqsghs------vynpsatgkelsgYTWSISYGDGSSASGNVf-t 87
gi 145606112  93 YLSPIMVGepaqMVWMNLDTGSSDTWMYasttgeghkgdqkf----yapevsktsthvpnTSWWIEYGDGTYASGDVw-k 167
gi 160705628  89 FYVPTVIGn--qTFKMLYDTGSADLWVIsedgpqwqadeghp-----lyhpsssaellpnHNWSIKYAYGQSASGVVy-l 160
gi 16944483  106 YLCEIGIGtpqqKVKLDFDTGSADLWVRctdssllhhadkkf----dpkksdtfqesktdQTWKIQYGDGSTASGTVg-t 180
gi 121703882  90 YLARVSIGtpeqRFNVAVDSAVGGLWVWstnlsaetiaghragtgaifdgeksstfsggeGVWRVEYGDGASAGGIVg-r 168
gi 46138607   87 FIADIKVGtppqTLKVAIDTGSADLPNAsktah-----------------------rvrgAEWEVEYIDGTHADGIVy-l 142
gi 83764571   89 FFMPVSIGtpaqTFNLVLDTASVDFWVRsselpadtlpkgeeeshtfdpeksstfkniedSPWKVTYVDGSSVSGSIg-t 167
gi 171689804  93 YLSRVGFGtpfqYLNVDLDTGSADVWVYssetktktrredif-----eleksstaelvngSEWRITYGDSSYAWGHVy-h 166
gi 39968217   91 WLNQVQVGtppqNLTVQLDTGSAEFWVFadthptakqagrgv----yspsnsstaalipgITWSKGYVGGDSASGDAvyn 166
gi 39973745  106 YLCEVKIGtpaqKLELQLDTASSDIWVFssgssrgvnrtnf------dawksssfscqsdKTWVMKRPDGSSISGEVg-t 178
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1PPL_E        88 DSVTVGgvt---ahgQAVQAaqqisaqfqqdtnNDGLLGLAFSSintvqp----qsqttFFDTVKSsla----qpLFAVA 156
gi 145606112 168 DTLSIGdve---iknMTIQTalmasvamvtdvnMSGLAGLCPNHpstvmps--qptlleKLEPVLDe-------fVFAAD 235
gi 160705628 161 DTVKAGplt---atkQAIEAatqis----fetaTDGILGLAPGIinqvqp----erqktFFETLAPtlq----rnLFAAN 225
gi 16944483  181 DVITVGglq---iknQAIELakkvss-afssgeADGLLGLAFSTintiesdgkpdpqptPVENMISqedipkeaeLFTSA 256
gi 121703882 169 EKIVLGemt---iqkQEFGLadvlsa-qfargtADGVLGLGFGSlgpdgpr-glveamcRTGDVSSsv------eLFTVR 237
gi 46138607  143 DTVRLGsfelkntpiQSAQIiaarf---esetgVTGIMGLAKTLpsnvepp--tpslldKLRPLLDk-------aVFTVD 210
gi 83764571  168 EDITIGgva---fktQPVQLaktmsp-mftqfsADGVLGLAFGHinhpqq---dikalaERLNTRDdreq--sakLFTAK 238
gi 171689804 167 DSIDIGgip---lhnAVVQSavdvsqslssdkdIDGIFGLAYDLhsqvr-----pkqptVLSTLKShld----kpVFTAD 234
gi 39968217  167 DTVRVGdlv---sqsQTILPataasa-syvnssYDGILGLDLADytatlpi-efkgppnFFEAIKPslp----apLFGVD 237
gi 39973745  179 DTVVVGgle---vpdQLLGLgkvvggtstermvADGTLGLSMPQtryiqrngvndpqtsFVARMAEakilpedaqLFTTA 255
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                    ####
1PPL_E       157 Lkh------qqPGVYDFGFIDssk---ytgSLTYTGVdns------qgFWSFNVdsytags-----qsgdgfSGIADTGT 216
gi 145606112 236 Lry------qdTGRFRFGHVPksd---yegEIHWARMnkt------skFWQFDInsvhvggt--nillqstwSFIADTGT 298
gi 160705628 226 Hrv------dgKGTWDFGYIDesk---ytgDITWAPVsgt------qkHWQVDVgeyavgqg--sfssepvgEVIVDSGS 288
gi 16944483  257 FysarddkseeKSFYTFGWVDedlvkasgkDITWTPIdns------egFWKFPSesatvdgd---nvsvsgnTAIADTGT 327
gi 121703882 238 Lgssg--aeaeESFCTFGFVDeetvkifggEVYYTPIdds------qgFWMFDSgsavvngk---tvaregnKAIVDSGT 306
gi 46138607  211 Lrr------naTGRFDFGYVDesr---akdKISWVSTrnd------shHWDVTFdmtawtgar-rvwmsrsfEATIDTGT 274
gi 83764571  239 Lgswgd-sdlkKSFCTFGSIDedavrycghDVHHTPIdns------rgYWMFESpsatvggk---pmsrsenKAVVDTDA 308
gi 171689804 235 Lryq-----sdEGAYTFGYIDhhr---higEINYTPLlpn------stFWEFNFtglhvvgh--nywyisqwRVIADTGT 298
gi 39968217  238 Lkq------dkESFFDFGVIPpnr---yrgDVGWTAInrfrvkdmdftRWNMTVsaysignvqtaplktrnmTGVADTGT 308
gi 39973745  256 Lcrptq-ggkqESFCTFGYVDedm---vdgEISWAKVdsg------kgMWMFPSasttindd---kisrsgnMAVVDSST 322
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1PPL_E       217 TLLLLDDSvvsqyysqvs-gaqqdsnagGYVFDCStn---lpDFSVSIsgytatvpgslinygps--------gdgstcl 284
gi 145606112 299 TLMLLPMDltkmyydqvp-gaeynewydSYTFPCNetk-nlpSWDFQIaglngtvpghyiaytnvt-------eklcygg 369
gi 160705628 289 VLIYLPEAvvkdyygqi--pgyvptqggSNTFPCNst---mpDLNFKVgsgvltvpgrevnygsyd-------kakgtcv 356
gi 16944483  328 TLALVSDTvckalyakip-gskysyryqGYLIPSTitadqlpQLSVAVggeqfviqnedlllapa---------dddhwy 397
gi 121703882 307 MLTLVDDEtcqriydaip-gayydeesqGYLFPSDtaanelpVVSLAVgerqfvvrkedlgfaea---------kpgyay 376
gi 46138607  275 TLIFLPADlagaywndvpnmrinpdlgdAFTFPCEfad-elpDLLFKVpktehvlkipgrylrystv----dddpeycwg 349
gi 83764571  309 ALTLLDDQtcqaiydsip-gafydsetqGFLIPSDinvdqlpVVQLAVgeksfamskeslmfaea---------kpgyiy 378
gi 171689804 299 TLMLLSPDivnmyydavpnatsdrsfggLWHYPCNtt---lpDFEIGFangwvarvpgrymnytty------ddlpgrcm 369
gi 39968217  309 TLMYLDKPvvqeyysqvp-gsrydstsaGWVFPCDggp--mpDFSFGVgpmesatvitvpgiylnwtvndqakrecfggl 385
gi 39973745  323 PLILLSDEaceafykkie-gakysrlrqGWLVPGGetq-gmpELRVAVgetefgistse--------------------y 380
                        330       340       350       360
                 ....*....|....*....|....*....|....*....|.
Feature 1                                                 
1PPL_E       285 ggiqsnsgigFSIFGDiFLKSQYVVFDSdg----pQLGFAP 321
gi 145606112 370 iqpwsaetygFGILGDvFLKAVYAVFDVqn----kTVGFAN 406
gi 160705628 357 gaitmqlnmkYSVLGSiWMRNYYVIHSNedg--alKMGFAL 395
gi 16944483  398 ggvqsrgtmpFDILGDtFLKSIYAIWDQgn----nRFGAVP 434
gi 121703882 377 ggiqsrgsmkMDVLGGtFLMGLYAIFDVgn----lRFGAVQ 413
gi 46138607  350 gmqsadglggVTIIGDmAIKAWYVAFDLea----nKVGFAN 386
gi 83764571  379 ggvqsrgsleFDVLGGtFLDGLYAIFDFg------SLRFGA 413
gi 171689804 370 gglqpfmseeFGILGDiFLKAVYAVFDIgg----gKVGFAD 406
gi 39968217  386 qeainfqgasISLFGTvAMKAAYVIFEDrrpdadpRIGWAV 426
gi 39973745  381 afspadgdmfFGVVQS-RGNNEFDVLGVpf----lRSVYAP 416

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