Conserved Protein Domain Family
citrate_synt_like_2

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cd06102: citrate_synt_like_2 
Citrate synthase (CS) catalyzes the condensation of acetyl coenzyme A (AcCoA) and oxalacetate (OAA) to form citrate and coenzyme A (CoA), the first step in the oxidative citric acid cycle (TCA or Krebs cycle). Peroxisomal CS is involved in the glyoxylate cycle. This group also includes CS proteins which functions as a 2-methylcitrate synthase (2MCS). 2MCS catalyzes the condensation of propionyl-CoA (PrCoA) and OAA to form 2-methylcitrate and CoA during propionate metabolism. This group contains proteins which functions exclusively as either a CS or a 2MCS, as well as those with relaxed specificity which have dual functions as both a CS and a 2MCS. The overall CS reaction is thought to proceed through three partial reactions and involves both closed and open conformational forms of the enzyme: a) the carbanion or equivalent is generated from AcCoA by base abstraction of a proton, b) the nucleophilic attack of this carbanion on OAA to generate citryl-CoA, and c) the hydrolysis of citryl-CoA to produce citrate and CoA. There are two types of CSs: type I CS and type II CSs. Type I CSs are found in eukarya, gram-positive bacteria, archaea, and in some gram-negative bacteria and are homodimers with both subunits participating in the active site. Type II CSs are unique to gram-negative bacteria and are homohexamers of identical subunits (approximated as a trimer of dimers). Some type II CSs are strongly and specifically inhibited by NADH through an allosteric mechanism. This subgroup includes both gram-positive and gram-negative bacteria.
Statistics
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PSSM-Id: 99856
View PSSM: cd06102
Aligned: 31 rows
Threshold Bit Score: 214.047
Threshold Setting Gi: 21224175
Created: 18-Oct-2007
Updated: 17-Jan-2013
Structure
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Aligned Rows:
  next features
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1           #                                                                            
gi 13473292   58 RGRSPSA--IAESAIaWGEPSIATAISTVWHGHLIYRGRDAAVLSDNAt-LEETAAVLWGLPEPirfeapapdaprqpgr 134
gi 162450007  66 DARAGHGp-VAAEALrWGEPVLESALTAIGPRGPVYRGKEAVALALDDvpFEAVAELLWTGELPaegapaarpgrlsvrp 144
gi 108757424  73 DARAGHAa-VASGALrWGEPVIDSSVSRVGAEGLAYRGHSAVTLAVEGraFEDVAELLWTGTLPeeasrwpspepafpps 151
gi 118591516  53 KGRKAADd-YSGARPlTGDPVLETELTLISETGPVYRGRPAVDLAQTSt-LETVATLLWASQEDpfaesaplpppdlpeg 130
gi 21224175   63 ARRNRRDggINPAPAaGQELTVRTRLTLIENDRFSYRGVDAVELAARHt-YEEVAEWLWTGELRpgaafsapessvavar 141
gi 91783094   58 RGREPRVa-TAESTMrWGEPVLCSAITEITTEGPVYRNRPAMELALSGvsFESVAQFLVTGVWQdrigiwpalslpddvm 136
gi 163841630  24 ARKRRTAs-APAALTgAPLMVLDSPFALLTEADLYYRGRLASELALRHs-FEQVASGLWSADSAslteqpikfsrpefde 101
gi 91784081   62 QIKSGIA--PASARDtAPGLTGSSAITLMTEAGSFYRGVSAIDLAEKAt-LEDVARLLWDAGDFdpfecpatalppqsda 138
gi 115376689 100 DARAGHAa-VASGALrWGEPVIDSSVTRIDARGVAYRGHLAVDLVAQGcsFEAVAELLWTGQLPasqvrwpspplpfpas 178
gi 108805348  64 EHRRDPAr-AAEGALdWGMPVLESGISLIADGRLYYRGRDAVALSGERs-LEQVAQLIWTGSMApgdraavpglfraaar 141
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 13473292  135 asafa--------------------------qlallagqarpslgrsaaslcADAARAIgvlagal------gaeagpDP 182
gi 162450007 145 aklaallpegappfvalalvvpalgahdatrhvgapsgdgriptgdaeraraRALVRSLaalvglgrdarratdaleaDG 224
gi 108757424 152 elakllprgtppv----------aalsalvpllgardsarfasppeqeraraRRLLRHLsawvcvanapgrvthalnePT 221
gi 118591516 131 lapldrlmv-------------------alaawplqdkaaytqapkllqvkgAALLRYAvaalln--------adpvdVP 183
gi 21224175  142 ravdalpehag--------------padrlrvaaiaaavtdplrfdlseeavLGTARVLiptlvaalp-pvlrdghdrDP 206
gi 91783094  137 llvdayaghlaaadv-------galfgmlafalgmqgrgatevadggtmrgaRQLIQAMagcmgflgpqrrfvtpnedES 209
gi 163841630 102 qkaaapsalgsdargtd--fmlqalliasshdpfkrdlspqsvaklgmhaiaYMIEALPskfanl-------apigstAS 172
gi 91784081  139 llkacgnfsald------------raimllpaieaanprahdlgkhgflrsgADVLRWFaawmlg-------qssparLP 199
gi 115376689 179 algallpsetppl----------rvlaavvpllgatdavrfdaspgqewlraRRLLRLMggwvgvghglhrvsaaleqDS 248
gi 108805348 142 svagcprlplggarp------vldelglalqaaasrdpaaydlrpaavvrtgARILRLLvgaatg--------eqdagET 207
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                       #  #                             #### #  #       
gi 13473292  183 VHRRLARGWSVDd---AGADAIRRALVLLADHELNASTFAARVAASTGASPAACLLAGLATLSGPRHGGAGEAVMQLAED 259
gi 162450007 225 AARRLLIALGANps-kKAERAVNRALLVSADHELNASTFAVRVAASTGADLYACVSAGLAALSGPRHGGMTDRVEALVAE 303
gi 108757424 222 VAQSLARAWNTRv--kRAPELLNRALVLCADHELNVSTFTARVAASSSADLYACLSAALAALSGPKHGGACDRVEALLVE 299
gi 118591516 184 IHTQIANAFGVSs---SAKNVIRAALILCADHELNTSAFATRCAASTRAPLHAALISGLGAFAGPRHGAASDRTTAWLSD 260
gi 21224175  207 IARRLWTRLSGQepdePTLQALDTALGLLVDHDLAASTLAVRVAASARAHAYAAVSAGLGVIEGPLHGAAGGLAHRMLLE 286
gi 91783094  210 IAAFLLRAAGGGqsrpDSTRIMNSALVLLADHELAPATFAARIAASTNADLFNCVATAIGSQEGSSTGTSTRNIETLLLP 289
gi 163841630 173 VSEVLWFKLTGRlaqaNEAALLNMVLVLLLDHDLAESTFAGRVAASARAHPYAALISALSTLDSSLRGSVSVAARELIVA 252
gi 91784081  200 LHRYIGATTKCGp---KLEDAVRRVLVLSADQALHPATYVARAAASTGATPYRCVIAGLSAATGKRLPAVRVGAFSRFLA 276
gi 115376689 249 LSAGLAQAWGVAv--rSAPALLDRALVLCADHELNVSTFAARVAASSGADLYACVSAALAALSGPRHGGSCDRIEALMRD 326
gi 108805348 208 AARALQRRWVPRd--eGAAEILDRALVLCADHELNVSSFTARCVASAGATPYAAVVAGLAALGGVRHGGGTERVEAFLAE 285
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                            ########  #    #                                 #  # #     
gi 13473292  260 Aarh--gaDAAIRRWLGhdrPLPAFGHPLYPDGDPRTTALSAAIPV-------DETLQQLEKAAIAMtGARPNIDFALAA 330
gi 162450007 304 Agrp-eraAAAVHERLRrgePIAGVGHRLYPEGDPRAQLLLDTARAlapqsaaLRTVLALVDALRDAgLDPPSLDLGLVA 382
gi 108757424 300 Vgrp-eraAEVIRERLRrgeAVPGFGHQLYPDGDPRTPPLVEAARSvrpetpnVRIASAVLEAMRAAgHPPPSVDFGLVM 378
gi 118591516 261 Vgpd-tdiETMLSDRLSrgePLPGFGHTIYRGPDPRAACLLDVLYRsepahpfVQRLPGLITVAHDLfGEAPNIDLALAA 339
gi 21224175  287 Vlel-gsaAPVVAEELRagrRIPGLGHRLYPGEDPRARALFALLEDvpraapaIAAARDVQATAARHtPLHANVDLALAV 365
gi 91783094  290 Rvvt-tdqERRLDLVREygaSLFGFNYPLYPAGDPRARLLLELISGlgspsdeTASLLEFLELARSGlEAYPGVAIALVA 368
gi 163841630 253 Vmag-qspERAISAKLNngyGIPGFGHRIYQRRGPRAATLLAALRElppyreaLNAAAAIIAVVRARtESPVNIDFALAV 331
gi 91784081  277 EidnaqdsTEPIRIRMReseDIPGFGYSPFNAPDPRSAALWASLRVlla--tdRNFARFERAIDLAIdLTGQQPDFALLA 354
gi 115376689 327 Vgrp-araASVVHERLRrgeSIPGFGHRLYPAGDPRTPPLLEVAWKvrperaaVRVARAVAEAMREEgHPPPTVDFGLVA 405
gi 108805348 286 Veaa-gdpREVVASRLRrgeAVPGFGHRLYPEGDPRAAALLAALAEgypgseaLGLAEGAAEAAGALvGERPNVDFALAV 364
                        330       340       350       360
                 ....*....|....*....|....*....|....*....|....*..
Feature 1                       #   #                #  #       
gi 13473292  331 LTRGLGLPrDAPFQLFALGRSVGWAAHMVEQIVS-GSIIRPRGRYEG 376
gi 162450007 383 IAAALGLPaGTAAALFAVGRAAGWIAHAFEQREA-GYMLRPRARYTG 428
gi 108757424 379 LTGALGLPsGAGATLFAVGRAAGWVAHVLEQREQ-GHLLRPRARYVE 424
gi 118591516 340 TQKTYGLPkDAGKIIFCAARMTGWIAHALEQYAA-PEQIRPRAAYVG 385
gi 21224175  366 LTVSSGMAsTAAETIFAVARTAGWIAHALEEYGErPLRMRPSGLYSG 412
gi 91783094  369 LTRALGLPdGAAIAIWIISRTAGWAAHVLEQRTQ-AFLLRPRAKHIE 414
gi 163841630 332 LIIGAEMPaDGGQTIFAVARSAGWLAHIMDEYRQaPLRLRPIGEYTG 378
gi 91784081  355 AYVNRRIGaEPDANLVRLGRIAGWVAHAWEQQNE-MPLVRWKVNYNG 400
gi 115376689 406 LAAALGLPpGAATALFAVGRAAGWVAHVWEQREQ-GHVLRPRARYVE 451
gi 108805348 365 LARVLGLPpGGALALFALGRAVGWVGHAIEQYAS-GAPIRPRARYVG 410

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