Conserved Protein Domain Family
EcCS_AthCS-per_like

?
cd06107: EcCS_AthCS-per_like 
Click on image for an interactive view with Cn3D
Escherichia coli (Ec) citrate synthase (CS) gltA and Arabidopsis thaliana (Ath) peroxisomal (Per) CS_like. CS catalyzes the condensation of acetyl coenzyme A (AcCoA) and oxalacetate (OAA) to form citrate and coenzyme A (CoA), the first step in the citric acid cycle (TCA or Krebs cycle). The overall CS reaction is thought to proceed through three partial reactions and involves both closed and open conformational forms of the enzyme: a) the carbanion or equivalent is generated from AcCoA by base abstraction of a proton, b) the nucleophilic attack of this carbanion on OAA to generate citryl-CoA, and c) the hydrolysis of citryl-CoA to produce citrate and CoA. There are two types of CSs: type I CS and type II CSs. Type I CSs are found in eukarya, gram-positive bacteria, archaea, and in some gram-negative bacteria and are homodimers with both subunits participating in the active site. Type II CSs are unique to gram-negative bacteria and are homohexamers of identical subunits (approximated as a trimer of dimers). Some type II CSs, including EcCS, are strongly and specifically inhibited by NADH through an allosteric mechanism. Included in this group is an NADH-insensitive type II Acetobacter acetii CS which has retained many of the residues used by EcCS for NADH binding. C. aurantiacus is a gram-negative thermophilic green gliding bacterium; its CS belonging to this group may be a type I CS. It is not inhibited by NADH or 2-oxoglutarate and is inhibited by ATP. Both gram-positive and gram-negative bacteria are found in this group. This group also contains three Arabidopsis peroxisomal CS proteins, CYS-1, -2, and -3 which participate in the glyoxylate cycle. AthCYS1, in addition to a peroxisomal targeting sequence, has a predicted secretory signal peptide; it may be targeted to both the secretory pathway and the peroxisomes and perhaps is located in the extracellular matrix. AthCSY1 is expressed only in siliques and specifically in developing seeds. AthCSY2 and 3 are active during seed germination and seedling development and are thought to participate in the beta-oxidation of fatty acids.
Statistics
?
PSSM-Id: 99860
View PSSM: cd06107
Aligned: 7 rows
Threshold Bit Score: 613.674
Threshold Setting Gi: 119481929
Created: 31-Oct-2007
Updated: 17-Jan-2013
Structure
?
Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 26 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                 #                                                                      
1NXG_A        39 GVFTFDPGFTSTASCESKITFIDGdeGILLHRGFPIDQLATDSnYLEVCYILLNGEKPTQEQYDEFKTTVTRHt-mIHEQ 117
2H12_A        46 GVFTFDPGYGETAACNSKITFIDGdkGVLLHRGYPIAQLAENAsYEEVIYLLLNGELPNKAQYDTFTNTLTNHt-lLHEQ 124
gi 15231130   91 GLKLYDPGYLNTAPVRSSICYIDGdeGILRYRGYPIEELAESStFIEVAYLLMYGNLPSQSQLADWEFTVSQHs-aVPQG 169
gi 83775953   58 GLRVYDPGYSNTAVSHSKITYIDGlkGTIQYRGYSINDIVGRKtFIDTAHLLIWGHWPSTAEAETLQQRLDQVp-vPQDF 136
gi 119481929  50 GLRVHDPGLQNTTVVETRISFSDHekGLLLFRGYTLEQLWDSD-FEDMLHLLVWGSYPTALQKKELSRKLSEEmtmVPKS 128
gi 145230211  87 GLRLIDPGYRNTAVKMSSITYINGneGVILYRGHPLASLIGKS-YEEITHLLIWGSLPTPEQRLRFQRRIAEAmmvVPEN 165
gi 163849332  44 GLMSYDPAYLNTASCKSSITYIDGdkGILEYRGYPIEQLAEQSsYLEVAYLLLYGELPSKERLAWWEYRISRHl-fLHNS 122
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1NXG_A       118 ITRLFHAFRRDSHPMAVMCGITGALAAFyhdsldv--------nnprhrEIAAFRLLSKMPTMAAMCYKYSIGQPFVYPR 189
2H12_A       125 IRNFFNGFRRDAHPMAILCGTVGALSAFypdandi--------aipanrDLAAMRLIAKIPTIAAWAYKYTQGEAFIYPR 196
gi 15231130  170 VLDIIQSMPHDAHPMGVLVSAMSALSIFhpdanpalsg-qdiykskqvrDKQIVRILGKAPTIAAAAYLRTAGRPPVLPS 248
gi 83775953  137 VFNVIKSFPRDGSLMGMVIAGLSALQSSdmnaipahvgktiylnnpelaDQQIIRVMANMSMLTAAAYCHHIGRDFTPPR 216
gi 119481929 129 VHRTIETLPRTTSPLPLMLAGLSACLAYapesipastkpdlyqsnsnvvDRAIIRTVAAYAVVFGLVNCHRRGIPFAQPS 208
gi 145230211 166 VKQLVATFPRNTPPMVILCAVLTGYLADqpelipahaganlynrrpemvDEQIIRTLAVTAIAGSIAHCHMKGEELRMAD 245
gi 163849332 123 LVELIQAFRYDAHPMGILISSVAAMSTLypeakni--------hdpavrEKQIWRIIGQIPTIAAFAYRHRIGRPFNLPD 194
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                #  #                              ### # 
1NXG_A       190 NDLSYAGNFLNMMFstpcepyeVNPILERAMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGAN 269
2H12_A       197 NDLNYAENFLSMMFarmsepykVNPVLARAMNRILILHADHEQNASTSTVRLAGSTGANPFACIAAGIAALWGPAHGGAN 276
gi 15231130  249 ANLSYSENFLYMLDsmgnrsykPNPRLARVLDILFILHAEHEMNCSTAAARHLASSGVDVYTACAGAVGALYGPLHGGAN 328
gi 83775953  217 AGLSYIENFLLMTGhveaatglPNPRYVNAIERLWVLIADHEMTCSTAALLQTASALPDVISCMVSAISALYGPLHGGAI 296
gi 119481929 209 RHKSYLENLFQMAGlvdqttgrPDPTKLSCFRRFAMLNADHGMALSVFSALVTTSSLTDPISCLITATGAAFGPLHFGAT 288
gi 145230211 246 PNLSYIENILWMGRyvdnnpavTREKAAEILTKAWSLYADHEMTNSTSAFLHVSSSLADPLSAMAACCMSGYGLLHGGAI 325
gi 163849332 195 SSLSYTANLLYMMDymnqreyeVNPVLAKALDVLFILHADHEQNCSTSVMRSVGSSHADPYNALAAAAAALYGPLHGGAN 274
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         #                            ########  #    #                                  
1NXG_A       270 EAALKMLEEIssvkhIPEFFRRAKDKndsfRLMGFGHRVYKNYDPRATVMRETCHEVLkelg--tkdDLLEVAMELENIA 347
2H12_A       277 EAVLKMLARIgkkenIPAFIAQVKDKnsgvKLMGFGHRVYKNFDPRAKIMQQTCHEVLtelg-ikddPLLDLAVELEKIA 355
gi 15231130  329 EAVLKMLAEIgtaenIPDFIEGVKNRk--rKMSGFGHRVYKNYDPRAKVIKKLADEVFsiv---grdPLIEVAVALEKAA 403
gi 83775953  297 EVAYKNIESIgsisnVPAKIARVKAGk--eRLYGYGHRVYRVTDPRFVFIREILNELSeev---ekdPLLKVAFEVDRVA 371
gi 119481929 289 ESANLALREIgtpekVPKFIEEVKQGk--rRLFGYGHRSYKGLDPRVAPIRSILKDLDts-----snSLIKVAERIEQVA 361
gi 145230211 326 DAAYRGMREIggpqnVPKLIEKVINKe--cRLSGYGHRIYKQVDPRAKYVREMLDELTrdrdiremdPVLQVAMEIDRIA 403
gi 163849332 275 EAVLRMLQQIghpknVPAFIERVKKGe--mRLMGFGHRVYKNYDPRAKIIRKIAHEVFaat---aanPLLDVAMELERTA 349
                        330       340       350       360       370       380
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
Feature 1                # #  # #                    #   #                 #  #       
1NXG_A       348 LNDPYFIEKKLYPNVDFYSGIILKAMGipssMFTVIAAMARTVGWIAHWSEMHSDg-mKIARPRQLYTG 415
2H12_A       356 LSDDYFVQRKLYPNVDFYSGIILKAMGiptsMFTVLFAVARTTGWVSQWKEMIEEpgqRISRPRQLYIG 424
gi 15231130  404 LSDEYFVKRKLYPNVDFYSGLIYRAMGfppeFFTVLFAVPRMAGYLSHWRESLDDpdtRIMRPQQAYTG 472
gi 83775953  372 SEDEYFTSRNLRPNADLFAAFVYKALGfppeFILPLSILSRTQGFMAHWREAMGNp-pRIWRPGQIYTG 439
gi 119481929 362 SADGYFRSRGLYPNADFYGNFVFTGIGfepeLIPAAMMSQRIMGVMAHWREYMCKslqESVRYRRVSDL 430
gi 145230211 404 STHEYFVKRNLQANADLYGSFVYTALGidsqFATVLAATARVSGVMAHWKEQTERa-pDLWRPLQVYVP 471
gi 163849332 350 LEDEYFISRKLYPNVDFYSGLIYQALRfpieYFPFLFAIPRASGWLAQWIEMLEDpeqKITRPRQVYVG 418

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap