Conserved Protein Domain Family
BSuCS-II_like

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cd06110: BSuCS-II_like 
Bacillus subtilis (Bs) citrate synthase (CS)-II_like. CS catalyzes the condensation of acetyl coenzyme A (AcCoA) and oxalacetate (OAA) to form citrate and coenzyme A (CoA), the first step in the citric acid cycle (TCA or Krebs cycle). 2MCS catalyzes the condensation of propionyl-coenzyme A (PrCoA) and OAA to form 2-methylcitrate and CoA during propionate metabolism. The overall CS reaction is thought to proceed through three partial reactions: a) the carbanion or equivalent is generated from AcCoA by base abstraction of a proton, b) the nucleophilic attack of this carbanion on OAA to generate citryl-CoA, and c) the hydrolysis of citryl-CoA to produce citrate and CoA. This group contains proteins similar to BsCS-II, the major CS of the gram-positive bacterium Bacillus subtilis. A mutation in the gene which encodes BsCS-II (citZ gene) has been described which resulted in a significant loss of CS activity, partial glutamate auxotrophy, and a sporulation deficiency, all of which are characteristic of strains defective in the Krebs cycle. Streptococcus mutans CS, found in this group, may participate in a pathway for the anaerobic biosynthesis of glutamate. This group also contains functionally uncharacterized CSs of various gram-negative bacteria. Some of the gram-negative species represented in this group have a second CS isozyme found in another group. This group contains proteins which functions exclusively as either a CS or a 2MCS, as well as those with relaxed specificity which have dual functions as both a CS and a 2MCS.
Statistics
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PSSM-Id: 99863
View PSSM: cd06110
Aligned: 18 rows
Threshold Bit Score: 564.978
Threshold Setting Gi: 159898555
Created: 18-Oct-2007
Updated: 17-Jan-2013
Structure
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Aligned Rows:
  next features
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1           #                                                                            
gi 56381113    5 RGLEGVVATTSSISSIIDDt--LTYVGYDIDDLAENAs-FEEVVYLLWHRELPTKEQLEELKKQLAENaeIPNEIIEHFk 81
gi 26006951    5 NGLKDMIACDTRISAIKDNk--LSYAGYDIADLMDNKarFEEVIYLLWNLHLPTAIELKQFEEKLRKNyaISDAIEQCIl 82
gi 13701491    6 RGLEGVIAAETKISSIIESq--LTYAGYDIDDLAENAq-FEEVIFLLWNYRLPNEEELAHLKGKLNQYmtLNPRVYTHFe 82
gi 149178483  15 KGLKGIIAADSSICLVNGTigkLLYRGYNIDDLAENAt-FEDVSFLLLNGELPTATQLAAYQAELKQYrsIPPELIDALk 93
gi 42522160   13 KGLEGVVACTTKVSFIVGDs--LNFRGYTIDDLAANSt-FEEVTYLLWNDKLPNAQELAAFSTQLRNEmaLSPDFIKVLk 89
gi 116749536  10 IGLRGVTVADTKISYIDGEngiLIYRGFRIEELAERSs-FMETAYLLLNGVLPSAEELREFTAEVDAArtVPEFVIDSMr 88
gi 118194517  83 IGLRGIPVADTRISNIDGKqgrLIYRGYDISDLSSKSn-FEEVAYLLLHDGLPTKDRLSAFNSRLVEArwIPKQMQINMg 161
gi 159898555 105 RGLAGVTFDQSQITLIDGKqgrLQYRGYSIHDLVEQTt-FEETAFLLLNGQLPTTTELDQFKHQLVAAreIPATILELIq 183
gi 163740487   9 RGLKGIYFERSGVSDIDGAkgeLSYRGYSIHDLAAHSt-FEEVCYLLIHGELPDADQLAEFDTALKAArvLPAPVLDIIr 87
gi 116748969  10 IGLRGITVADTKISYIDGVrgiLIYRGFRIEDLAEISs-FEETAYLLLNGGLPDKEGLAAFRDALTAAgrLPDEISESFk 88
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 56381113   82 lypidkVHPMAALRTAVSLL-GLYDeeadvmtkeanYRKAIRLQAKIPTIVTAFARVRKGLEPVAPRKDLSFAANFLYML 160
gi 26006951   83 iqsrqhLHPMSVLRSTVSLL-GVYNlkaeersveatYDQSIQLMAKIPTIIATFARLRQGLSPIAPRKDLGFAANFLYML 161
gi 13701491   83 eyvtdhVHPMTALRTSLSYI-AHFDpdaenesdenrYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYML 161
gi 149178483  94 kl-pvsAPPMALLRSITSLA-GVYDpnaeietfenrLQISIKLISQIPTIVAAIHRVRQGLEPVEPDPELCHAGNFMYMI 171
gi 42522160   90 ai-ptnVHPMGWLRTAVSLM-AHWDsdandngaeanLRKSVRLTAKMGTLLCAFDAIRKGKEPVAPKADKSMAWNMMYML 167
gi 116749536  89 ri-preADPMDVLQGVVPFL-GVADpelmvetreanLRKAVRLIARVPAVVAAWNRIRNGLEPLPPDEGLPHAANFLWQL 166
gi 118194517 162 nw-rkdADPMDMLQAFVAALaGYYDeefan--keasYDRAINLIGKVPTIVASWHRIRNGEDLIDPDPALSHAANFLHMM 238
gi 159898555 184 ll--kdGHPTEVLRTCLSAL-ATFDaerldgsvtatKARSIRLIAQMPCLIAAHHAIRQGKTPIQPNPTLDHAANFLYML 260
gi 163740487  88 at--kdGHPMDVLRTAVSAL-AALEpnsqqvgedafVANGIRLISQVPIIIAAHDAIRNGREVVAPDMTLSHAGNWLWML 164
gi 116748969  89 lw-prrSDPMDIIQASVPIL-AMIDpelaaesreanLRKAIRVIARLPEIITTWHRMRNGLQPLPHDPKLGHAGNFLWKL 166
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                               #  #                              ### #  #               
gi 56381113  161 t-GKEPddIATEAFNKALVLHADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANEAVMKMLTEI----GTV 235
gi 26006951  162 n-GRLPseLEILAMNRALVLHAEHELNASTFAARVCASTLSDIYSCVTTAIGTLKGPLHGGANERVFDMLREIr---EYG 237
gi 13701491  162 r-GELPtdIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPLHGGANEQVMTMLSEI----GSI 236
gi 149178483 172 n-GEKPseDATRAMDLILTLHAEHGLNASTFTGRVIIATLPDIYSAVTGAIGALKGKLHGGANTEVLKTLFEI----GSV 246
gi 42522160  168 ggGAEPkaEHVKVMDTCLILHADHELNCSAFATRVTASSLSDLHSAIVSAIGALKGPLHGGANEQVILMLQKI----GTM 243
gi 116749536 167 t-GNKPddQIARDLDVCLVLHADHTFNASTFACREVVSTQAHMYAGVSAGVGALSGSLHGGANARVMKMLFEVqkevKSE 245
gi 118194517 239 t-GKVPdkEMERIFDTCLILHADHTFNASTFAARQVASTRAHMYSAASAAIGALSGELHGGANYEVMRMLLDM----GSV 313
gi 159898555 261 q-GTIPsqQAQQIVNQILILHADHSANASTFAARVVAGTRSDWYAALTAAIAAFAGPLHGGAIEQVIAMIQAI----GTP 335
gi 163740487 165 k-GEKPtpEATRLADVDFILHAEHGANASSFAARVTIGTETNLHGGIVTALSTLAGPAHGGAAEDVMKMVHEI----GTP 239
gi 116748969 167 t-GKKPeeEMARAFEVSLILQADHAFSASTFACREVVSTRAHLYAGVTAGVGALSGILHGGSNALVMRVMFDMidqgMTT 245
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                       ########  #    #                                        # #  # # 
gi 56381113  236 DnvEPYIRRKLANKEKIMGFGHRVYRKGDPRAKHLKKMSEKLtklvGEPHWYEMSTKIEEIVTSE------KALPPNVDF 309
gi 26006951  238 Dv-DSYLQEKLNSKEKIMGFGHRVYQTQDPREKYLREMARELtkgtEHDIWYQLSKKVEICMKQK------KNLIPNVDF 310
gi 13701491  237 EnvDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSRQItkdaGREELFEMSVKMEKRMAEE------KGLIPNVDF 310
gi 149178483 247 EnvAPYVKKVREAKGKFMGFGHAVYQVEDPRAKHLKELSRRLgeetGEPKWYEMSIEMEKLVYEE------IKRNCNVDF 320
gi 42522160  244 EkaQQFVKDALAAKEKVMGIGHRVYKNGDPRARILRGMSDKLtkdaGIHHMYEMSTLIDDTMFNE------KGLMPNVDF 317
gi 116749536 246 DevGDWVRMRMERGEKIMGMGHAVYKTFDPRSKILKEMSLRLgvrtGHEDWYRLLTRIEQEALKEfekkgkDNIKSNVDF 325
gi 118194517 314 EavVPWIRKRMDDGQRIMGMGHAVYKTYDPRATVLKELSRRLaa-kTGQPWFAITEKVEQATADEmkkrkgVEIYPNVDL 392
gi 159898555 336 EraADYVANLQANNQPVMGFGHRVYQTEDPRARHLRKAAQALsa-qSDNNYYAILEAVVQAMRPYm----aKGIDVNVDF 410
gi 163740487 240 DnaAAYVKTKRAAKEAVTGFGHRVYRKEDPRARHMRDGVRQLgaemGAPEWYEILQAVVEAMQPYa----rHGLNVNVDF 315
gi 116748969 246 KdiGAWVKGKLDRREKIMGMGHAVYKTTDPRAEILKGLCENVaiktEREEWFNFLRAIETECIREfekrgkRKIKANVDF 325
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|....*
Feature 1                           #   #                    #  #       
gi 56381113  310 YSASVYHCLGIDHDLFTPIFAVSRTSGWLAHILEQYDN-----NRLIRPRAEYTG 359
gi 26006951  311 YSATVYHVLGIDSSIFTLIFAMSRVSGWIAHIQEQQKN-----NKLIRPRSHYTG 360
gi 13701491  311 YSASVYHCMEIPHDLFTPIFAVSRSAGWIAHILEQYKD-----NRIMRPRAKYIG 360
gi 149178483 321 YSASLQHYMGIPGDLFTCIFAASRIAGWCAHILEQLDG-----NKIIRPKANYIG 370
gi 42522160  318 YSATVYFSMGIPTDLFTPIFAASRISGWCAHAFEQYAN-----NRIYRPRGKWAG 367
gi 116749536 326 YSGSVYSMMGIPVDIMTPVFAISRISGWCSHVIEEKFAeaqdkPALYRPKAEYVG 380
gi 118194517 393 YSASVYYMLDIPMDLNTPIFAISRVAGWAAHIIEEKFAeaapkPALYRPKAVYVG 447
gi 159898555 411 YASVIYHLLGIPYDLFVPAFIVGRTVGWLAQIQEQYAN-----NILIRPLLAYVG 460
gi 163740487 316 YSGVIYQLHGIAMDLYVPIFAIGRMPGWIIQCIEQQRG-----NILIRPLTLYNG 365
gi 116748969 326 YSGLLCAMLGIPLDIMTPVFAMAIAVGWCSHIIEEKFGdaqgkPVLYRPESKYVG 380

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