1A59


Conserved Protein Domain Family
DsCS_like

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cd06111: DsCS_like 
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Cold-active citrate synthase (CS) from an Antarctic bacterial strain DS2-3R (Ds)-like. CS catalyzes the condensation of acetyl coenzyme A (AcCoA) and oxalacetate (OAA) to form citrate and coenzyme A (CoA), the first step in the citric acid cycle (TCA or Krebs cycle). 2-methylcitrate synthase (2MCS) catalyzes the condensation of propionyl-coenzyme A (PrCoA) and OAA to form 2-methylcitrate and coenzyme A (CoA) during propionate metabolism. The overall CS reaction is thought to proceed through three partial reactions: a) the carbanion or equivalent is generated from AcCoA by base abstraction of a proton, b) the nucleophilic attack of this carbanion on OAA to generate citryl-CoA, and c) the hydrolysis of citryl-CoA to produce citrate and CoA. DsCS, compared with CS from the hyperthermophile Pyrococcus furiosus (not included in this group), has an increase in the size of surface loops, a higher proline content in the loop regions, a more accessible active site, and a higher number of intramolecular ion pairs. This group contains proteins which functions exclusively as either a CS or a 2MCS, as well as those with relaxed specificity which have dual functions as both a CS and a 2MCS. For example, included in this group are Corynebacterium glutamicum (Cg) PrpC1 and -2, which are only synthesized during growth on propionate-containing medium, can use PrCoA, AcCoA and butyryl-CoA as substrates, and have comparable catalytic activity with AcCoA as the major CgCS (GltA, not included in this group).
Statistics
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PSSM-Id: 99864
View PSSM: cd06111
Aligned: 9 rows
Threshold Bit Score: 662.954
Threshold Setting Gi: 117928865
Created: 28-Mar-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 26 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1          #                                                                            
1A59_A        7 KGLAGVTADVTAISKVNSdtnsLLYRGYPVQELAAKCsFEQVAYLLWNSELPnDSELKAFVNFERSHrkLDENVKGAIDL 86  Antarctic bacter...
P26491       14 KGLAGVVVDTTAISKVVPetnsLTYRGYPVQDLAAQCsFEQVAYLLWHGELP-TDQLALFSQRERASrrIDRSMQALLAK 92  Mycobacterium sm...
NP_969318    20 KGLDGVVMDTSSVSKVNPhtnsLIYRGYPVQDLAENCsFEEVAFLMYNGELPnAGQLAEFSKKERSYreISTTLLNVIKA 99  Bdellovibrio bac...
YP_251460    41 KGLNGVVVDTSSVSKVNPetnsLTYRGYPVQELAEHCtFEDVAYLLWNGELPdFRELEQFRQRERALrpIDRSMLDLINS 120 Corynebacterium ...
ZP_01017275   9 YGLAGVLADDTAISKVMPetnsLTYRGYAVQDLSANCrFEDVAYLLWNGDLPsKTDRERLDQQERSQraVSDDVLEVMSR 88  Parvularcula ber...
YP_266563     7 KGLLGIVVDETEISKVMPeinsLTYRGYAAQDLCARCdFEEVAYLILNKELPnKKQLKEFKKELSKEitLSKNLLSILKK 86  Candidatus Pelag...
Q8NSH7        8 KGLNGVISDYTSISKVMPesnsLTYRGYAVEDLVENCsFEEVIYLLWFGELPtTEQLRTFNTTGRSYrsLDAGLISLIHS 87  Corynebacterium ...
YP_873416    11 KGLVGVYADTTAVSTIDEptntLIYRGYPAAQLAERYsIEHIAYLLWYGDLPtAAEFDAFTSRIRAAyrLDPAVADWLAT 90  Acidothermus cel...
Q8NSL1       11 KGLYGVIADYTAVSKVMPetnsLTYRGYAVEDLVENCsFEEVFYLLWHGELPtAQQLAEFNERGRSYrsLDAGLISLIHS 90  Corynebacterium ...
Feature 1                                                                                       
1A59_A       87 LstaCHPMDVARTAVSVLGanharaqdsspeanLEKAMSLLATFPSVVAYDQRRRRGEELIEPREDlDYSANFLWMTFge 166 Antarctic bacter...
P26491       93 LpdnCHPMDVVRTAISYLGaedleedvdtaeanYAKSLRMFAVLPTIVATDIRRRQGLTPIPPHSQlGYAQNFLNMCFge 172 Mycobacterium sm...
NP_969318   100 LpqkCHPMDSIRTAVSFLGaedariwdaspatnMDKAMMLLAKIPTMVAADYRFKKGLDFIPPKADlSIAENFFHMCFgk 179 Bdellovibrio bac...
YP_251460   121 LpktAHPMDVLRTAVSVFGtedlnaddrspdavRRTGLTLLAKLPTIIAYDLRRRKNLDYIPPSRDrDIAENFLYMCFgd 200 Corynebacterium ...
ZP_01017275  89 FskhAHPMDTLRTAVSFLGqedpttedssasglMEKSIRMYARIPTIVAADFRRRNGKDPIAPSDVlGFSENFFHMCFge 168 Parvularcula ber...
YP_266563    87 IpknSHPMDVARTAVSVMGledketkdsspkanLRKAIRILAKTPTALAAFYRLRKGKKIIAPKKNlTFSGNFFHMCFgk 166 Candidatus Pelag...
Q8NSH7       88 LpntCHPMDVLRTAVSYMGtfdpdpftrdadhiRSIGHNLLAQLPMVVAMDIRRRSGEEIIAPDHNkGIASNFLSMVFgn 167 Corynebacterium ...
YP_873416    91 TrpdTHPMDLLRTAVSILGatdsstpqldrdttLAQATRLLAQMPAVVAAIQRHRHAQPPIPADPGlGIAENFFHMCFdd 170 Acidothermus cel...
Q8NSL1       91 LpkeAHPMDVMRTAVSYMGtkdseyfttdsehiRKVGHTLLAQLPMVLAMDIRRRKGLDIIAPDSSkSVAENLLSMVFgt 170 Corynebacterium ...
Feature 1                               #  #                              ### #  #              
1A59_A      167 e-----aapEVVEAFNVSMILYAEHSFNASTFTARVITSTLADLHSAVTGAIGALKGPLHGGANEAVMHTFEEIgirkde 241 Antarctic bacter...
P26491      173 v-----pepVVVRAFEQSMVLYAEHSFNASTFAARVVTSTQSDIYSAVTAAIGALKGSLHGGANEAVMHDMLEIgsa--- 244 Mycobacterium sm...
NP_969318   180 v-----pqkEVVKAFDVSLILYAEHSFNASTFTARVVTSTQSDIYSATVAGIGALKGPLHGGANEMVMHMMKEIadp--- 251 Bdellovibrio bac...
YP_251460   201 eegspannrDDVEAFDQSLTLYAEHGFNASTFTGRVISSTMSDVYSAVTGAIGALKGPLHGGANEAVMHNFLAVedp--- 277 Corynebacterium ...
ZP_01017275 169 v-----padEIVKCFDISMTLYAEHSFNASTFTARTIASSLSDVYSAVTGAIGSLKGPLHGGANEAVMHMLKEIgep--- 240 Parvularcula ber...
YP_266563   167 v-----pekEIVKAFDVSLILYAEHSFNVSTFTARTITSSLSDIHGAITGAIASLKGPLHGGANEEVMHMMKKIkkp--- 238 Candidatus Pelag...
Q8NSH7      168 ddgsvansaDDIRDFERSLILYAEHSFNASTFSARVISSTRSDTYSAITGAIGALKGPLHGGANEFVMHTMLDIddp--- 244 Corynebacterium ...
YP_873416   171 i-----psePMVRAFETSLILYAEHGFNASTFTARVVTSTLADLYSAITAAIGALKGPLHGGANEAVMYMLQQIdsp--- 242 Acidothermus cel...
Q8NSL1      171 gpespasnpADVRDFEKSLILYAEHSFNASTFTARVITSTKSDVYSAITGAIGALKGPLHGGANEFVMHTMLAIddp--- 247 Corynebacterium ...
Feature 1                            ########  #    #                                     # #  #
1A59_A      242 sldeaatrsKAWMVDALAQKKKVMGFGHRVYKNGDSRVPTMKSALDAMikhyd---rpemLGLYNGLEAAMEEAKQIKPN 318 Antarctic bacter...
P26491      245 ------ekaPEWLHGKLSRKEKVMGFGHRVYKNGDSRVPTMKVALEQVaqvrd---gqrwLDIYNTLESAMFAATRIKPN 315 Mycobacterium sm...
NP_969318   252 ------akaEQWMLDALAQKKKVMGFGHRVYRSGDSRVPTMKKYAQVMadvtg---eqkwMQMYTALEKVMVDKKKIYPN 322 Bdellovibrio bac...
YP_251460   278 ------aaaEQWVLDQCDAKNKIMGFGHRVYKKGDSRVPTMEAAMRRTaanhd---gqrwVDMYDNMQKAMDQRTGIQPN 348 Corynebacterium ...
ZP_01017275 241 ------aeaKQWMLDAIAEKKKIMGFGHRVYRSGDSRVPTMTKAYEKMvevidtpeakkyWEMSRILDDTMVDAKGIYPN 314 Parvularcula ber...
YP_266563   239 ------enaLKWITKALKNKDVVMGFGHRVYKSGDSRVPTMREYFKRVaiikk---dktfEKIYDIVEKVMIKEKNIYPN 309 Candidatus Pelag...
Q8NSH7      245 ------nnaADWMGKALDRKERIMGFGHRVYKNGDSRVPSMEKSMRSLaarhr---gqkwVHMYESMQEVMEARTGIKPN 315 Corynebacterium ...
YP_873416   243 ------eaaADWVRRALERKERIMGFGHRVYKHGDSRVPAMEKALKTVvaerg---atdlLARYHAVQDAVYQAKQLLPN 313 Acidothermus cel...
Q8NSL1      248 ------nkaAAWINNALDNKNVVMGFGHRVYKRGDSRVPSMEKSFRELaarhd---gekwVAMYENMRDAMDARTGIKPN 318 Corynebacterium ...
Feature 1        #                    #   #               #  #             
1A59_A      319 LDYPAGPTYNLMGFDTEMFTPLFIAARITGWTAHIMEQVADNALIRPLSEYNGpeqRQV 377 Antarctic bacterium DS2-3R
P26491      316 LDFPTGPAYYLMDFPIESFTPLFVMSRITGWTAHIMEQAASNALIRPLSEYSGqpqRSL 374 Mycobacterium smegmatis
NP_969318   323 LDFPAGPAYYMMGFEIDFFTPIFVMARTTGWSAHIMEQTADNRIIRPLSEYVGaeqRKV 381 Bdellovibrio bacteriovorus HD100
YP_251460   349 LDFPAGPTYYMLGIDIPFFTPIFVMARVVGWTAHIVEQNEDNALIRPLSSYVGpeqRPV 407 Corynebacterium jeikeium K411
ZP_01017275 315 LDFPAGPAYYLMGFEIPMFTPIFVMSRITGWTAHVMEQLANNKLIRPLSNYSGvaqRDV 373 Parvularcula bermudensis HTCC2503
YP_266563   310 VDYPTGPTYHLMGFDTDFFTPIFVISRITGWSAHIMEQHAANKLIRPLASYKGskhRKV 368 Candidatus Pelagibacter ubique HTCC1062
Q8NSH7      316 LDFPAGPAYYMLGFPVDFFTPLFVLARVSGWTAHIVEQFENNALIRPLSAYNGveeREV 374 Corynebacterium glutamicum
YP_873416   314 LDFPAGPAYYLMGFDIPIFTPIFAMARVTGWVAHVLEQRADNALIRPLAVYTGptsRPL 372 Acidothermus cellulolyticus 11B
Q8NSL1      319 LDFPAGPAYHLLGFPVDFFTPLFVIARVAGWTAHIVEQYENNSLIRPLSEYNGeeqREV 377 Corynebacterium glutamicum

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