Conserved Protein Domain Family
citrate_synt_like_1_2

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cd06113: citrate_synt_like_1_2 
Citrate synthase (CS) catalyzes the condensation of acetyl coenzyme A (AcCoA) and oxalacetate (OAA) to form citrate and coenzyme A (CoA), the first step in the oxidative citric acid cycle (TCA or Krebs cycle). Peroxisomal CS is involved in the glyoxylate cycle. This group also includes CS proteins which functions as a 2-methylcitrate synthase (2MCS). 2MCS catalyzes the condensation of propionyl-CoA (PrCoA) and OAA to form 2-methylcitrate and CoA during propionate metabolism. This group contains proteins which functions exclusively as either a CS or a 2MCS, as well as those with relaxed specificity which have dual functions as both a CS and a 2MCS. The overall CS reaction is thought to proceed through three partial reactions and involves both closed and open conformational forms of the enzyme: a) a carbanion or equivalent is generated from AcCoA by base abstraction of a proton, b) nucleophilic attack of this carbanion on OAA to generate citryl-CoA, and c) hydrolysis of citryl-CoA to produce citrate and CoA. CSs are found in two structural types: type I (homodimeric) and type II CSs (homohexameric). Type II CSs are unique to gram-negative bacteria. Type I CSs are found in eukarya, gram-positive bacteria, archaea, and in some gram-negative bacteria. Type I CS is active as a homodimer, both subunits participating in the active site. Type II CS is a hexamer of identical subunits (approximated as a trimer of dimers). Some type II CSs are strongly and specifically inhibited by NADH through an allosteric mechanism. This subgroup includes both gram-positive and gram-negative bacteria.
Statistics
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PSSM-Id: 99866
View PSSM: cd06113
Aligned: 16 rows
Threshold Bit Score: 694.012
Threshold Setting Gi: 158439952
Created: 18-Oct-2007
Updated: 17-Jan-2013
Structure
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Aligned Rows:
  next features
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1           #                                                                            
gi 6456029    28 KGLRDINGKGVLAGLTNISAIHSFdkEGNQi-----pGVLEYRAYNIKDIVSDLrsknRFGFEEMTYLLLFGELPSAKEL 102
gi 118725346  34 KGLRNSDGSGVLVGLTEIGEVHGYf-ISEGerk-edeGHLVYRGIDVYEMVRGFqkekRFGFEEVCYLLLFAKLPNRDEL 111
gi 52217902   32 RGLRNEDGTGVLVGLTKIGNVVGYe-RIPGgglkpipGKLFYRGYDLEDLAHAIlkekRFGFEEVAYLLLSGSLPDKEEL 110
gi 158439952  32 KGLRNEDGTGVRIGLTRVADVVGYe-ETDGgik-aipGRLLYRGVDVLDLVKGKke-sHFGYEETCFLLLFGYLPKKAEL 108
gi 156869319  34 RGLRNPDGTGVMAGLTHICNVHGYl-IADGeki-pdeGRLTYRGINVKDIVDGCtrenRFGYEEVAWLLLFGTQPTREQL 111
gi 154499087  38 RGLRNADGTGVLAGITQIGNVLGYy-VQDGerf-pmeGKLIYRGIDLNDLVNGFmsenRFGFEETAYLLLFGALPTREQL 115
gi 139438890  57 RGLRNADGSGVVAGLTRISDVHGYn-KVDGkvv-pdqGKLTLRGYSIEDLVNNAqaenRYGYEEVAYLLITGSLPNKEEL 134
gi 158432279  30 KGLRNEDGTGVRVGLTRIADVVGYe-IRNEekc-dvaGKLYYRGIELRELINGYskaqTYGYEEICFLLLFGYLPNKAQM 107
gi 167772631  42 RGLRNADGTGVLAGITQICNVHGYl-LSEGekv-pidGELTYRGYSIRDLIAGAqaggRYCFEETAWLLLLGFLPDGMQL 119
gi 167750122  30 RGLRNNDGSGVVVGLTNICCVHGYv-IDEGekr-papGQLIYRGYDICDLISGVekdgRYGFEETAYLLLFGNLPDKEHL 107
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 6456029   103 QEFQILLASKrtLPEFFVRETIltnPSSDVMNSMSRCLLALASYDkkasDISIENvLEQSLALIADFPLLAIYSYQAYIH 182
gi 118725346 112 SEFTELLGGLraLPVGFTEDMIlkaPSCDIMNKLARSVLASYSYDdepdGIDPKNlLRQSIELIARFPTMAAYGFQAKSH 191
gi 52217902  111 ASFRELINDNmpLEQKTKMNIIe-lEGNNIMNILARSVLEMYRFDpnpdDTSRDNlMRQSIDLISKFPTIIAYAFNMLRH 189
gi 158439952 109 EKFCNVVRHCytIPDEFVEMNLlrlPGKNLMNKLQDAVLTLYNYDddpdNIDVYQtLVKGVNLLAKLPSIACYAYQSKVH 188
gi 156869319 112 EKFNRILSSYreLPEYFAEDMIikaPSKDIMNKLGRSVLALYSYDsnpdETSLENiMRQSISLIARMPTIMTYAYQVKRR 191
gi 154499087 116 TLFQRALSVHqpLPPMFTEDMIlraPSGDVMNKLARSVLSLYSYDdapeDRSLESeMGKAIALIARLPTIAAHAYAAKRH 195
gi 139438890 135 DGFCERLGAFrhLSDEYVKEFPmttVSSSIMNVLQRAVLLLYAFDaepdVITPEHeIDVAISMLARLPRIAAFAHMASVA 214
gi 158432279 108 KEFCELLRSHyeLPKDFLETKFlqmPGKNIMNRIQQAVLSLYDYDdkadATDISNtLLQGIDILAKLPSILVYAYQSKLH 187
gi 167772631 120 RRFFDILSDAraLPDNFTEDMImkaASPNIMNMLARSVLALYTYDpapeDTSIENvMLQSIQLIARMPSIMAAAYQVKKR 199
gi 167750122 108 EDFKKIVTHYrtLPPYFAEDVLmrcPSSNIMNKMAQSVLALYTYDkepeNKSMESeMLKAISVIARLPAIMVGAYQVMRR 187
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                         #   #                          
gi 6456029   183 YFkkeSLYIHFPDPElTTAENILRMLRpdchYSDMEAKVLDIALILHMEHGGGNNSTFTTHVVTSSGTDTYATIAAALSS 262
gi 118725346 192 YYggqSLYIHSPLPQlSTAENILHMIRpdnkYTQTEAEILDLALVLHAEHGGGNNSTFAARVVSSTETDTYSAIAAAVGS 271
gi 52217902  190 ATfgrSLHIRHPQENlSIAENFLYMLKr--dYTELDARTLDLLLVLQAEHGGGNNSTFTVRVTSSTGTDTYSAIAAGIGS 267
gi 158439952 189 YYdreSLFIHYANPEySIAENFLSLLRkdqkFTSKEANLLDTILMLHADHGGGNNSTFTNVVIASTGTDIYSTVCGGIGS 268
gi 156869319 192 HYdkkSMYFHPIKPEhSTAESILRSIRpdkkFNDEEAKLLDLLMIIHAEHGGGNNSTFTTRVLSSSGTDTYAAISAAIGS 271
gi 154499087 196 YFdneSLYLHRPQEGlGVAENFLYSVRhdnqFTEEEAKLLDLCLVLHAEHGGGNNSAFACRVLSSSGTDIYSAIAAAIGS 275
gi 139438890 215 KLrgsEVHVPHPTPGlSTAETILQVLRggmaFTRDEAMLLDVMLMLHAEHGGGNNSTFACRVLSSSATDPYSAYAAAIGS 294
gi 158432279 188 RYhqeSLVIHLVQHNlSIAENLLYMLRkdnrYTKLEAQLLDMLLIVHADHGGGNNSTFTNVVIASTGTDIYAAMVGAIGA 267
gi 167772631 200 VYdgrSMYLHNPNPEfSTAQNILRMLRsdkqFTEDEAHLLDLCLTLHSEHGGGNNSTFAVRVLTSSRTDTYSAISAGIGA 279
gi 167750122 188 YLynsSMVMHPINLDeSLAESILSTLRdnreYTKEEAKLLDLCLMLHAEHGGGNNSTFTCRCVTSTGTDAYAAYASAISA 267
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            ### #  #                                         ########  #    #           
gi 6456029   263 LKGPKHGGANIKAAKMLENIken--isDYNNDeei-kkYLHQILNKEVFDkQGLIYGIGHAIYSVSDPRFEVFKSYVESL 339
gi 118725346 272 LKGPKHGGANIKAMNMLSDIkeh--vkDWTDDdev-rnYLIKIINKEAFDkAGLIYGMGHAVYTLSDPRTILLKEKAHQL 348
gi 52217902  268 LKGPLHGGANIQVADMFHHLqen--iqDWTNVdei-dtYFTRMLNKEVYNkTGLIYGIGHAVYTISDPRAVLLKELARDL 344
gi 158439952 269 LKGPRHGGANIKVAEMMDAVlaetggyDADDKki--rqAVEKLMAGELYDrSGLVYGFGHAVYTVSDPRAEVLRACCMEV 346
gi 156869319 272 LKGPKHGGANHRVMQMMECVkek--vkDWKDEdev-eaFLEKIVRKEEGDhSGLIYGQGHAIYTLSDPRAVILKTHAQRL 348
gi 154499087 276 LKGPRHGGANKKVMEMFGYIedg--vkDWRDDdei-rsYLSRILHKEAGDgSGLIYGMGHAIYTLSDPRAVLLKKFAKSL 352
gi 139438890 295 LKGPRHGGANAKVVSMHEDIrah--vsNWEDEdev-aaYLGKILDKQAFDgTGLIYGMGHAVYTLSDPRAEVCRRYARSL 371
gi 158432279 268 MKGPRHGGANMKVRAMLEAVve---kiGYCEEeevicnLINQILDKRFFDgSGLVYGMGHAIYTLSDPRSEVLQSYIEIL 344
gi 167772631 280 LKGPRHGGANFKVIEQLEYLkan--vrNWDDDgei-rdYLRRVVRREAGDrSGLIYGMGHAVYTKSDPRERLLKENALRL 356
gi 167750122 268 LKGPRHGGANIKVMEMLNCIeng--vkNWENEgev-adFLTKILNKEENDhSGLIYGMGHAVYTLSDPRAVQLKKSALKL 344
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                       # #  # #                    #   #                
gi 6456029   340 AKEKGleeEFKLYEKVAFLAPKVisenrkiyKTICPNVDFYSGFVYKILEIPQELFTPLFAIARIVGWLAHRMEELINm- 418
gi 118725346 349 AIEKGrekEFLLYDAVERIAPDVfaqakgttKNICANVDLYSGFVYDALGIPTELFTPLFAVARIVGWCAHRIEEVVSm- 427
gi 52217902  345 AHEKGrerEFAFLELLEERAIATfgkiknngKTVSSNVDFYSGFVYEMIGLPQEIYTPLFAMARIVGWCAHRNEELNFeg 424
gi 158439952 347 AKEQKhtrELDFYMRFERIAKEVmee--ikgKALPTNVDFYSGFAYGMLRIPRDLYTPLFVCSRVVGWVAHNIENKLYd- 423
gi 156869319 349 AKEKGmddEFALYQLVEKLAPKAflnvkgdkKVISANVDFYSGLVYKMLGLPEDLYTPMFAVSRIAGWCAHRIEEVETs- 427
gi 154499087 353 AAKKGmldEFELYEAVERLTPEVlsas-gqrKVVCANVDLYSGLVYKMMGIPQELYTPLFAVARVVGWCAHRIEEVYNpg 431
gi 139438890 372 AAKKDlgeEFALIERIERLAPQVmrdh-gmtKPICANIDLYTGFIYKMLGVPETLFTPLFAVARMAGWSAHRMEELFCa- 449
gi 158432279 345 AKEKQrmsEYAFYKRFEACAKKVmwe--rkhIRISSNVDFYSGFVYDMLGIPQELFTPLFVLARTVGWLAHNIENKLYd- 421
gi 167772631 357 ARGTEfeaEFCLLDAIERLAPDVlhekqggdKPICANIDLYSGLVYRMLGIPDELHTPLFACARTAGWCAHRLEELSTg- 435
gi 167750122 345 AKGTEveaEFRLLDTIERLTPQLfaknkgdiKTMCANVDMYSGLVYKLLGIPVEMYTALFACARMPGWCAHRMEEIENg- 423
                        410
                 ....*....|..
Feature 1         #  #       
gi 6456029   419 NKIIRPAYESIL 430
gi 118725346 428 QKIIRPAYKSIS 439
gi 52217902  425 KRIIRPAYKNVL 436
gi 158439952 424 GRIMRPATKYVG 435
gi 156869319 428 NRIIRPAYKSLV 439
gi 154499087 432 NKIIRPAYKAVA 443
gi 139438890 450 HRIIRPAYRPVI 461
gi 158432279 422 GRIVRPATKYVG 433
gi 167772631 436 GKIVRPAFKSIV 447
gi 167750122 424 KRIMRPAYRTLI 435

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