Conserved Protein Domain Family
EcCS_like

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cd06114: EcCS_like 
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Escherichia coli (Ec) citrate synthase (CS) GltA_like. CS catalyzes the condensation of acetyl coenzyme A (AcCoA) and oxalacetate (OAA) to form citrate and coenzyme A (CoA), the first step in the citric acid cycle (TCA or Krebs cycle). The overall CS reaction is thought to proceed through three partial reactions and involves both closed and open conformational forms of the enzyme: a) the carbanion or equivalent is generated from AcCoA by base abstraction of a proton, b) the nucleophilic attack of this carbanion on OAA to generate citryl-CoA, and c) the hydrolysis of citryl-CoA to produce citrate and CoA. There are two types of CSs: type I CS and type II CSs. Type I CSs are found in eukarya, gram-positive bacteria, archaea, and in some gram-negative bacteria and are homodimers with both subunits participating in the active site. Type II CSs are unique to gram-negative bacteria and are homohexamers of identical subunits (approximated as a trimer of dimers). Some type II CSs including EcCS are strongly and specifically inhibited by NADH through an allosteric mechanism. Included in this group is an NADH-insensitive type II Acetobacter acetii CS which has retained many of the residues used by EcCS for NADH binding.
Statistics
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PSSM-Id: 99867
View PSSM: cd06114
Aligned: 33 rows
Threshold Bit Score: 666.206
Threshold Setting Gi: 15553143
Created: 1-Nov-2007
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 26 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                       #                                                
1NXG_A        18 LDVLKGTLGQDVIDIRTLgs-kGVFTFDPGFTSTASCESKITFIDGdeGILLHRGFPIDQLATDSn-YLEVCYILLNGEK 95
2H12_A        24 MPVLSGTLGPDVIDIRKLpaqlGVFTFDPGYGETAACNSKITFIDGdkGVLLHRGYPIAQLAENAs-YEEVIYLLLNGEL 102
gi 15838136   18 LPVLKPTLGNGCVDIAKLtketGLFTYDSGFTATASCKSAITYIDGekGVLLYRGYPIEQLAEHSs-FLEVAYLLMNGEL 96
gi 145495800  46 LPVYKGTKGPPMIDITQLysktGYFTLDPGFSSTGACMSTMTYIDGdkGELLYRGYPIETLAKYSs-YIEVCYLFIYGHL 124
gi 121608374  24 FPVLTPTNGPEMLDIGDLhkstGVCTLDIGLMNTGICKSAITYVNGdeGILRYRGYSIDDLVDNCd-YLDVAWLLLNGEL 102
gi 151571271  23 LPVYSPSLGNDCIDVSSLvk-hGIFTYDPGFMSTAACESKITYIDGgkGVLLHRGYPIEEWTQKSn-YRTLCYALIYGEL 100
gi 152993967  19 YDIMDGTRGPSVVDLRSFfadtGMFTYDPGYTSTASCSSQITFIDGakGELRYRGIPIEKLATEHs-YLETCYLLLNGEL 97
gi 98376125   16 LPILYDTEGNEVLDITSLykntGVLTYDPGFMSTAACESKITFIDGnkGILRYRGYDIADLVYNNksFIEVVYLLFYGVF 95
gi 58584992   17 LPVLSGTMGYDVLNIKDLykvtGLLTYDPGFVSTASCSSAITFIDGdeGVLRYRGHDIADLAQSNd-FTAVIYLLLYGKL 95
gi 15553143   16 LPVLRGTDGTPVVDITTLyrenNVLTYDPGFMSTAACRSEITFIDGnkGTLRYRGIDIENLIGTPnsFSSIVYLLLNGTL 95
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1NXG_A        96 PTQEQYDEFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYhdsldvnnprhrEIAAFRLLSKMPTMAAM 175
2H12_A       103 PNKAQYDTFTNTLTNHTLLHEQIRNFFNGFRRDAHPMAILCGTVGALSAFYpdandiaipanrDLAAMRLIAKIPTIAAW 182
gi 15838136   97 PRKDEFAKFDDEITHHTMMHESLKNFLGGFHYDAHPMAMLAASVASLSAFYhdtldlndleqrRLAAIRLIAKVPTLAAA 176
gi 145495800 125 PSQKELQLFEETMVSEMMINEKLIEFYKGFASDSHPMAIMVGVVGALSAFMhkdfdvndprdrEYIAIKLVAKMPTLAAY 204
gi 121608374 103 PTPSQKAEFQYNITYHSMVNEQMAYFLRGFRRDAHPMAVMCGMTGALSAFYhdeldifnaehrKIVAHRLIAKMPTIAAM 182
gi 151571271 101 PTDEQVKSFRQEIINKMPVCEHVKAAIAAMPQHTHPMSSLIAGVNVLAAEHihngq---kesqDEVAKNIVAKIATIAAM 177
gi 152993967  98 PSKEALQDFDLELRHRAYIHENLRLLFDAFPNNAHPMATLSATITALASFHkehltldndadyHTMASRLLAKMPTIAAF 177
gi 98376125   96 PNAEQLQEFILHISQEYVLPQMVRDVITSFPQNSHPMAILIASFSSLAAYYcdqk-----tdgELECKLAVAKVASIVAL 170
gi 58584992   96 PNLEQHKKFFLKIQELSKVPEQVVNVIKAFPKTAHPMSILIACFATLSALYherygnnvsnenLDFGISAIAQVPAIIAM 175
gi 15553143   96 PSETEHEEFARILGAEYDVPEQVMNVIRSFPRDSHPMAILIASFSALAANYhasr-----idpLTGAIIAIAKVPGIVAS 170
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                      #  #              
1NXG_A       176 CYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPcep--------yeVNPILERAMDRILILHADHEQNASTSTVRTAGSSGA 247
2H12_A       183 AYKYTQGEAFIYPRNDLNYAENFLSMMFARMsep--------ykVNPVLARAMNRILILHADHEQNASTSTVRLAGSTGA 254
gi 15838136  177 AHRYSIGWPIRYPRNNLGYVERFLHMMFEVPsep--------lqLNPVVTKALDLLFILHADHEQNASTSTVRLVGSTGA 248
gi 145495800 205 AYRTALGLPIIYPNKKYSFIENFLYMMFSTPmnd--------fnVDKVIVKALDTIFMLHADHEQNASTSTVRIAGSSLA 276
gi 121608374 183 AYKYSIGQPLVYPRNDLDFVSNFMQMLFAVPcep--------fkLNPAGVRAFSAVMIVQADHEQNASTFTVRSVGSSRA 254
gi 151571271 178 AYRHNHGKKFLEPKMEYGYAENFLYMMFADDes---------ykPDELHIKAMDTIFMLHADHEQNASTSTVRLSGSTGN 248
gi 152993967 178 AYRHSLGIPFIQPDIDLSFTKNFLTMMRAYPggkmhlgpngheeIKEVEVRALDTIFTLHADHEQNASTTVVRGVASTGA 257
gi 98376125  171 IYRYITNQDFIQADVGLSYSKNFIHMMFDISsy----------kFTEIVDKALDVIFVLHADHEQNASTATVRMTGSSGP 240
gi 58584992  176 VYRHTNDQEFIDTDNELNYSKSFLRMMFGNAld---------ndKSTLFAKALDKIFTLHADHEQNASTAAVRVVGSAGS 246
gi 15553143  171 IYRHTANLDFIQADANLEYTHHFIRMMFGDMdd----------aHRDIMHKALDAIFIMHADHEQNASTSTVRMTGSSGA 240
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                        ### #  #                              ########  #    #          
1NXG_A       248 NPFACIAAGIASLWGPAHGGANEAALKMLEEIssvkhIPEFFRRAKDKnd--sfRLMGFGHRVYKNYDPRATVMRETCHE 325
2H12_A       255 NPFACIAAGIAALWGPAHGGANEAVLKMLARIgkkenIPAFIAQVKDKns--gvKLMGFGHRVYKNFDPRAKIMQQTCHE 332
gi 15838136  249 NPYASVAAGITALWGPAHGGANEAVLKMLEEIgdaknVDLVIAKAKTKdknspfRLMGFGHRVYKHFDPRAKIIREMAHT 328
gi 145495800 277 NPFACIAAGITALWGPYHGGANEAVLNMLKEIgnkenIPKYLQKAKSKdn--sfRLMGFGHRVYKNYDPRAKVMQEMCYN 354
gi 121608374 255 NPYACVAAGIASLWGPLHGGANEGVLHTLNEIgsienIPAIIKRAKDKnd--pyRLMGFGHRVYKTYDPRAKVLQRYCYE 332
gi 151571271 249 SPYAAIIAGITALWGPAHGGANEAVLKMLSEIgstenIDKYIAKAKDKdd--pfRLMGFGHRVYKNTDPRATAMKKNCEE 326
gi 152993967 258 HPYVAISSGIAALWGRAHGGANESVIAQLEMIgsvenVEKYIARAKDPdd--dfRLMGFGHRVYKNFDPRAKILKTLQDE 335
gi 98376125  241 NLFACLAAGAATLWGPAHGGANEAVINMLMNIgkpenVKQFIQKVKDSnk--stKLMGFGHRVYKNYDPRARIMCTICKE 318
gi 58584992  247 NLFACLSAGVATLWGPVHGGANESVINMLKEIeqsgnVDKFIEKAKDDkd--pfKLMGFGHRVYKNYDPRARILKDACDE 324
gi 15553143  241 GLFACLCAGVATLWGPAHGGANEAVIKMLAEIgspenVSSFIDKVKNNkg--ksRLMGFGHRVYKSYDPRARVLRTICKD 318
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                       # #  # #                    #   #                
1NXG_A       326 VLkelg-tkdDLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPssMFTVIAAMARTVGWIAHWSEMHSDg- 403
2H12_A       333 VLtelgikddPLLDLAVELEKIALSDDYFVQRKLYPNVDFYSGIILKAMGIPtsMFTVLFAVARTTGWVSQWKEMIEEpg 412
gi 15838136  329 VLgelg-vndPLLEVALKLEEAALKDDYFVQRKLYPNVDFYSGIIYKAINIPleMFTVMFAIARTAGWVSHWLEQQVDpe 407
gi 145495800 355 VLektk-ranDILELAIQLEQTALKDDYFIQRKLYPNVDFYTGIVYEALSIPtsMFTVMFAVQRSIGWICQWMEMMSEkv 433
gi 121608374 333 VLeaygkkddPLFKMALELERIALNDEFFIERKLYPNVDFYSGLILKTIGIPlsMFTAQFAVARTAGWISHWNEMITDpa 412
gi 151571271 327 ILaklghsdnPLLTVAKKLEEIALQDEFFIERKLFPNVDFYSGIILKAMGIPedMFTAIFALARTSGWISQWIEMVNDpa 406
gi 152993967 336 LKeelq-ldsRLMEIAGKIEEIALRDEYFVSRNLYPNIDFYTGIILTALQIPknMFTPIFVIGRTVGWITQWIEFKKDpq 414
gi 98376125  319 ILxdfg-lndPLLEVAVELENIALQDDYFIARNLYPNVDFYSGIVLKTIGIPvkMFTTLFALARTSGWSAQWYEMVSSkn 397
gi 58584992  325 ILnkle-qdsKLLKIAKELEKIALKDEYFVVRKLYPNVDFYSGIIMNAINIPpsMFTPIFALARTTGWVTQWYEMINDke 403
gi 15553143  319 VLdslg-rdeNLLAVAEELESQALQDEYFIERKLYPNVDFYSGIVLRAMGVPvdMYTAFFALARTSGWASQWYEMIQSde 397
                        410
                 ....*....|...
Feature 1          #  #       
1NXG_A       404 -mKIARPRQLYTG 415
2H12_A       413 -qRISRPRQLYIG 424
gi 15838136  408 -mKISRPRQIYTG 419
gi 145495800 434 -qRISRPRQLYVG 445
gi 121608374 413 -vKIVRPRQLYVG 424
gi 151571271 407 -qKIGRPRQLYTG 418
gi 152993967 415 -sKIARPRQLYTG 426
gi 98376125  398 -yKISRPRQLYTG 409
gi 58584992  404 -tKICRPRQLYVG 415
gi 15553143  398 gkRISRPRQLYTG 410

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