Conserved Protein Domain Family
DNA_pol_III_epsilon_like

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cd06130: DNA_pol_III_epsilon_like 
an uncharacterized bacterial subgroup of the DEDDh 3'-5' exonuclease domain family with similarity to the epsilon subunit of DNA polymerase III
This subfamily is composed of uncharacterized bacterial proteins with similarity to the epsilon subunit of DNA polymerase III (Pol III), a multisubunit polymerase which is the main DNA replicating enzyme in bacteria, functioning as the chromosomal replicase. The Pol III holoenzyme is a complex of ten different subunits, three of which (alpha, epsilon, and theta) compose the catalytic core. The Pol III epsilon subunit, encoded by the dnaQ gene, is a DEDDh-type 3'-5' exonuclease which is responsible for the proofreading activity of the polymerase, increasing the fidelity of DNA synthesis. It contains three conserved sequence motifs termed ExoI, ExoII and ExoIII, with a specific Hx(4)D conserved pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis. The epsilon subunit of Pol III also functions as a stabilizer of the holoenzyme complex.
Statistics
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PSSM-Id: 99834
View PSSM: cd06130
Aligned: 55 rows
Threshold Bit Score: 186.563
Threshold Setting Gi: 126725765
Created: 3-Mar-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #### #                                                                      
gi 158335553  10 FVAIDFETADYqrDSACAIGVIRVENDQIVDRSHFLIRPP--RQKFQFTYIHGITWSDVKHHPTFAELWPNLQPKFAGsD 87
gi 154493796   4 FAAIDFEIANGkrSSICSIGVAIIEDNKVIDSVYMLVRPYpnFYTRFTTAIHGITMQDTIDSPDFEEAWTRIAGRLQD-I 82
gi 34540075    4 FAAIDFETANAerTSVCSVGIIVVRGGQIVHRYYSLIRPEpdYYNYHNTRVHGLTAADTESARIFPDVWAEVEPLIAG-L 82
gi 149199563   3 FVAIDFETATAkrNSACAVAIITVEKGVIVDEYSELIQPPnnEYSWRNIDVHGIEPRHTMYKEDFEDLYPEIRKRLTG-K 81
gi 87302916   26 FVALDFETANYsrDSVCSLAIIRVESLKVVSRTYRLIRPP--SPHFMFTDIHGITWGDVKSEPTFKELWHEFRPLFEGiS 103
gi 144898742   3 FIALDFETADNgrDSACSLALAVVKNGTVAETWHRLIRPP--RASQMFTHIHGITWDMVKDQPDFGHFWPEIRDLIDAaP 80
gi 94267095    4 FAAIDFETANYrrESACAVGVVLVEQGRIARRIYHLIRPP--SSWFVFTHIHGLGWEDVRRAPSFGEIWPEIAAELAGvE 81
gi 110636436   3 FTALDFETANThkTSICAVGMAIVEDGKVVKTISKLIKPVpdFYSYWNVQIHGITPDMTADAPYFIDVWQELKQYIDY-S 81
gi 168702572  18 FVAIDFETADHgpDSACAIGLARVENNKVVRREAVLIRPP--RPRVMFTEVHGITWPMVQNASPFGDVWRGLLPIIEGaK 95
gi 150025263   3 FTAIDFETATA--FHPCSVGIVTVQNGVIVDEFVTLIKPPnnQYSPFTIAVHGIRPRDTVNAKSFLEVYPEIKKRLQN-K 79
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            ## ###                                                         #    #       
gi 158335553  88 FVAAHNaNFDRSVLNACCQAAELDPM----PLPFLCTVKLAREVWNi-RPTKLPNVCEFLDIPLQ-HHQALSDAEACAKI 161
gi 154493796  83 PLVAHNsSFDEGCLRAAHEAYDLAYP----KYQFYCTCRLSRRMYPflANHQLQTVAAHCGYDLTyHHHAMADAYACAHI 158
gi 34540075   83 PLVAHNkPFDEGCLKAVFRMYRMDYP----DYPFFCTLQAARRQLRhlSDHRLPTVAQCCGYTLAqHHQALADAEACAAI 158
gi 149199563  82 LVVAHNeSFDRSVMTRSMEYYDLDYAdlgvADRWVCTVKLYKAQGF--KPAKLNILCDHFGIPLK-HHDALSDARACAEL 158
gi 87302916  104 FIAAHNaSFDSSVMRACCDTYRIVPP----GAPYLCTVKLARQTWQl-RPANLPSVCAYLGLHLE-HHNALSDAEACANI 177
gi 144898742  81 FLAAHNaPFDKGVLAECCRAHGHGAP----LTPFVCTVRVARDTWNl-RPTKLPDVCRFLAQPLN-HHDALSDAVACAEI 154
gi 94267095   82 FLAAHNaSFDRGVLNACCHAHGLAPP----SLPFVCTVQLARQRWNi-RPTKLSDVCRRLDIALN-HHQADSDAEACARI 155
gi 110636436  82 TLVAHNaAFDVGALRSILTTHEVSFP----KTDYFCSCSVSRKVFKeiENHKLSTVCRHIDVPLN-HHEAESDAIGAAQI 156
gi 168702572  96 FLAAHNaSFDRRVLHACCIAAGLDVP----ALPFVCTVQIARRKWAl-KPANLPAVCRRLGIGLI-HHHAGSDAEACARI 169
gi 150025263  80 TVVAHNeSFDRNVLAKSMALYGLDYEtlniSNRWECTLKIYKAKGF--KPTKLSDCCQEMNIKLN-HHEALSDARACAKL 156

                 ...
Feature 1           
gi 158335553 162 VIA 164
gi 154493796 159 AAT 161
gi 34540075  159 ALK 161
gi 149199563 159 YKR 161
gi 87302916  178 VRA 180
gi 144898742 155 MLA 157
gi 94267095  156 VIA 158
gi 110636436 157 IIK 159
gi 168702572 170 ILA 172
gi 150025263 157 YLL 159

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