Conserved Protein Domain Family
ERI-1_3'hExo_like

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cd06133: ERI-1_3'hExo_like 
Click on image for an interactive view with Cn3D
DEDDh 3'-5' exonuclease domain of Caenorhabditis elegans ERI-1, human 3' exonuclease, and similar proteins
This subfamily is composed of Caenorhabditis elegans ERI-1, human 3' exonuclease (3'hExo), Drosophila exonuclease snipper (snp), and similar proteins from eukaryotes and bacteria. These are DEDDh-type DnaQ-like 3'-5' exonucleases containing three conserved sequence motifs termed ExoI, ExoII and ExoIII, with a specific Hx(4)D conserved pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis. ERI-1 has been implicated in the degradation of small interfering RNAs (RNAi). 3'hExo participates in the degradation of histone mRNAs. Snp is a non-essential exonuclease that efficiently degrades structured RNA and DNA substrates as long as there is a minimum of 2 nucleotides in the 3' overhang to initiate degradation. Snp is not a functional homolog of either ERI-1 or 3'hExo.
Statistics
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PSSM-Id: 99836
View PSSM: cd06133
Aligned: 115 rows
Threshold Bit Score: 92.6685
Threshold Setting Gi: 116494067
Created: 28-Feb-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #### #                                                  #                   
1W0H_A        12 ICIIDFEATCeegnp----pefvhEIIEFPVVLLNTHTle-iEDTFQQYVRPEintQLSDFCISL--TGITQDQVDRADT 84
gi 89894542   16 FLVVDFEFSVpqsygk--prawfpEIIEVGATVLDSNGklalDKTFNAFVKPRfwpRLAEESYGI--TGIRQEDVDQGIL 91
gi 2231221     6 LLIIDFEFTMpdgkys--pqnffpEIIEAGIVKSIDDEv---VETFSSYVRPKkfpKLTKRCKSF--LKITQKQVDEGMR 78
gi 163814599  11 YVFVDFEMNLidsehkqekkicksEIIEIGAVKLDDEYn--eIDSFKSYVKPAy-gNMASRIIKL--TGITDEMLETAPS 85
gi 118443420  18 LLAIDFEFITtkivenk-akkyfqEIIEVGVIFKSESL----TQKYSKVIKPRyflNSKDKSKSIygGRFSYDDIKNGEE 92
gi 168695851   4 KLFIDFEFNIlddnknkpkeyngaELISIGGVLVDNEFn--tIDNYYSLVKPKynkILSNKCKNL--TKLNQLDINNAPN 79
gi 168697180   3 RVYIDFEMNMpnsksk--ravfnsDIIAIGAVMYDEKTk--nIDKFKSLIRPVsneELYPHIQEL--THISSEELKSAPS 76
gi 118727703   2 LIFVDFEATCwdknek--ykqphaEIIEIGAVATGKDLk--eIGKFSAVVKPTiepLLSDYCKEL--TGLSQTDVENGIE 75
gi 163816457  12 HIVVDLEMNWldfkyenerkistnEVIEIGAVTMNEKFe--eIGAFKMYVKPQfneKVVKKVEKL--TGITMDKLQDSPV 87
gi 164686250   3 KIYMDFEMNMnntknk--regfkaDLIAIGAIKYDTKTk--kIEKFKSLIKPIltkTVYPHIEEL--THITTEDLENAPT 76
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                   #####        #                                       
1W0H_A        85 FPQVLKKVIDWXKLkelgtkykYSLLTDGSWD-----XSKFLNIQCQLSrlk-------ypPFAKKWINIRKSYGNFYkv 152
gi 89894542   92 LEEAIQHLQKLAHPe-------AYVVAWGDAD-----RKILGNVCEKYGlk--------ypFVWENYIDLAEQYKHYRsl 151
gi 2231221    79 FEDFIRKLNELDPEk------nSTIITWGNMD-----MKVLKQNCMFNHip---------fPFKGEMRDLSLEYKNFFgd 138
gi 163814599  86 YEDAVGRFIEWCADa-------DVIYAWSEND-----LRQLRGETRLKQythp-----eleRVFAKWQDFQKEYARMIgt 148
gi 118443420  93 INKVFLELQQLYTPk------eTLWISWGKAE-----YDILKTVCNKYGin--------lpFLKEDYLDLSLEFKEFYgi 153
gi 168695851  80 LLYVMDDFYKWFCK--------FDDVTIYNWGdf--dITGLLTSFRVYKytg------kclELFNMMIDIQPFISQHIty 143
gi 168697180  77 YEEVMRKFKKWLGIfs----diKGIYTFGNLDltcfnNTDMRSAKKNNHprfvnnirdlfvDIKEKYINCGMRCMNYI-- 150
gi 118727703  76 FNRAIDLFYQWIIQyq-----dTDTPILYTWG-----NYDGFLLKRNLRrhrnknkdflkiIQKGGVANLQEKFLEFTkl 145
gi 163816457  88 FADAFDAFIKWCISf-------GEEVVMYGWS-----DSDELQLRREMKlknipw-kedvdKVFRQWHDFQKEFSDLVgi 154
gi 164686250  77 YESVMRSFKHWLGDfn----eiDGIYTFGNLDltcfkNTDRISSQKNNHprf-------lnNIQNFFVDIKEKYLEYGvk 145
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....
Feature 1                                  #    #            
1W0H_A       153 pr----sqtKLTIXLEKLGXdydgrpHCGLDDSKNIARIAVRXL 192
gi 89894542  152 d-----hliSLKKAIEENSIeqigilHSALDDAINAAQVMARMM 190
gi 2231221   139 r-----tltGLWKAAEEYGDsgtgthHKAHDDALTAYKLFKLVE 177
gi 163814599 149 s-----rriSLSDAVFYLGEnfqgaeHDALWDARNTAEVFKLSK 187
gi 118443420 154 g-----qnaSLDKALNYLNIpii-nrHYAIFDAQALMSIIHKMF 191
gi 168695851 144 nnrilskqiSLLNMKKIFSVegd-ikHNSLSDAVDLMNVCKCFF 186
gi 168697180 151 ---------SLKNLLEFVNLefsgdaHDPLNDAYNLFILDEAIT 185
gi 118727703 146 pc----sscSLTKALQIIGEdyrgikHRSINDAANMIKLYKYLG 185
gi 163816457 155 g-----riiTLSQAVDYAGIdfagqkHDACCDARNTAYLLALTK 193
gi 164686250 146 cm----nyiSLTHLLELSNMkfsgdaHDPLYDAYNLHILDEILE 185

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