Conserved Protein Domain Family
DEDDh_RNase

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cd06137: DEDDh_RNase 
Click on image for an interactive view with Cn3D
DEDDh 3'-5' exonuclease domain of the eukaryotic exoribonucleases PAN2, RNA exonuclease (REX)-1,-3, and -4, ISG20, and similar proteins
This group is composed of eukaryotic exoribonucleases that include PAN2, RNA exonuclease 1 (REX1 or Rex1p), REX3 (Rex3p), REX4 (or Rex4p), ISG20, and similar proteins. They are DEDDh-type DnaQ-like 3'-5' exonucleases containing three conserved sequence motifs termed ExoI, ExoII and ExoIII, with a specific Hx(4)D conserved pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis. PAN2 is the catalytic subunit of poly(A) nuclease (PAN), a Pab1p-dependent 3'-5' exoribonuclease which plays an important role in the posttranscriptional maturation of pre-mRNAs. REX proteins are required for the processing and maturation of many RNA species, and ISG20 is an interferon-induced antiviral exonuclease with a strong preference for single-stranded RNA.
Statistics
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PSSM-Id: 99840
View PSSM: cd06137
Aligned: 17 rows
Threshold Bit Score: 166.302
Threshold Setting Gi: 82231230
Created: 9-Jan-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #### #                                                         #   #         
1WLJ_A          8 VAMDCEMVGLGph---------------reSGLARCSLVNv----HGAVLYDKFIRP--EGEITDYRTRVSGVTp----- 61
gi 82231230   970 VGLDAEFVTLNeeeaelrsdgtkstikpsqMSVARITCVRgqgpnEGVPFIDDYIST--QEQVVDYLTQYSGIKpgd--- 1044
gi 116182686  197 VVLDCEMAGTSa-----------------gDYPIAVSVVDf---fTGAVLVDALVRPgaGVVVQDWRSGITGIGaa---- 252
gi 154296804  222 VTVDCEMAGTSgg----------------tGEVVMLCVIDy---iTGAVLLHRFVCP--REKITQMRSSIHGISkstl-d 279
gi 145243646  310 VVIDCEMVQAEkg----------------rRVIAYLAAVDf---iTGEVLINSYVDP--QRRIFNWCTRYSGITlkam-k 367
gi 46114532    84 IALDCEMAGVRng----------------nDEIISICVIDf---fTGQVLINSLVEP--HEPILEWRTDIHGIGpati-a 141
gi 154270618  365 VAIDCEMGQAAsg----------------dSELIRITLIDy---fSSAVLIDSLVYP--SVKMEHYRTRFSGVNrrdm-e 422
gi 169608982  398 VAIACSKVVLAdg----------------lQEVVKIAAVDv---vTCRILMNHLICNngTARVKNWRSDETGLYswddme 458
gi 67528318   369 VVLDCEMVGVVgpnn------------sevSEVVRLAAVDf---lSGEVLVNTFVEPaaDQRVINWRTRVSGVNksll-s 432
gi 85079405   150 VAIDCEMGVSSdf----------------eSELIRLSLIDy---fSGQVLIDRLVRP--NVPMQHMNTRYSGVTrqdl-q 207
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                ## ###                                   
1WLJ_A         62 -qHMVGATPFA---------VARLEILQLLKG--KLVVGHDLKHDFQALKedmsgYTIYDTSTDRLLWREakldh----- 124
gi 82231230  1045 ldAKISSKHLTt-------lKSTYLKLRFLIDvgVKFVGHGLQKDFRVINlmvpkDQVIDTVYLFHIPRKrm-------- 1109
gi 116182686  253 riADAGRAGSGdgeplagreGAREAVLRFVDGe-TVVVGQAVRFDLRALGiv--hGRVVDSAVLAAEASGrfggvagggg 329
gi 154296804  280 naNLQGQALSGw-------eGARSELWKYIDDh-TILVGHALQHDLDALRii--hPRIVDSGILSRNAVGirr------- 342
gi 145243646  368 kaVANGDTLRGw-------mGARRALWDFIDSd-TVLIGHDIKHDLNCLGii--hPRIVDSTILTAEAVFrprwetq--- 434
gi 46114532   142 iaASQGQVLRGw-------eAARQELFKHINMq-TILVGQSLYHDLKVLRvs--hEKIFDTAIHTAEMVFgtdaa----- 206
gi 154270618  423 aaKTAGSCIMGr-------dNARFAVWRYVGPe-TVVVGHSAHNDLESLRwi--hTAVVDTYIIEDLLEKkkncesk--- 489
gi 169608982  459 qaRKLGYKIFKgw------cAARSALSKFIDKd-TIILGHDLRSDLDCLRmi--hGRAVDIAKVAEKAAKgpls------ 523
gi 67528318   433 emKKQGQTVQGw-------eAARDLLWMFVDKq-TILIGHSLHNDLAVLGmv--hQRIVDSAILTRDAVAgake------ 496
gi 85079405   208 naIRNGTCIIGgl------eEARKEVWKFVGPq-TVVIGHSVRNDLSALRwi--hNRCVDSLVVEEGVRKkikakeeeee 278
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
1WLJ_A        125 -----------------------------------------------------crrvSLRVLSERLLHKSIQns------ 145
gi 82231230  1110 --------------------------------------------------------iSLRFLAWYFLDLKIQg------- 1126
gi 116182686  330 ------------------------------------------------vhrkikwwaGLEELCRELLGMRIRgg------ 355
gi 154296804  343 ------------------------------------------------------iqwGLQTLCSELLNIKIRnk------ 362
gi 145243646  435 ---------------------------------------------------rfrrtwSLKTLSCVFLDRFIQgg------ 457
gi 46114532   207 ----------------------------------------------------fgrrwSLQSLCMDLLGLRIRqn------ 228
gi 154270618  490 ---------------------------------------------------kdadktKDGKVAVETDKLLLLagepagne 518
gi 169608982  524 -----------------------------------------------------kpqlSLDSLCRDFNGVVLKsd------ 544
gi 67528318   497 ------------------------------------------------------dcaRVRKLAKTFFELDIQt------- 515
gi 85079405   279 kekekvrekqkeerlleislhcgpgmaeetrrmmeeeeikekekmkkmrgkrgedgmSLKALTKKKLGRVIQda------ 352
                         250       260
                  ....*....|....*....|..
Feature 1                #    #         
1WLJ_A        146 ----lLGHSSVEDARATMELYQ 163
gi 82231230  1127 -----ETHDSIEDARTALQLYR 1143
gi 116182686  356 ---egQSHDSLEDVLATRELVL 374
gi 154296804  363 ---kgGIHDCFEDVLATRDVVL 381
gi 145243646  458 ----sNGHSALEDAVATKDVVM 475
gi 46114532   229 ----sSTHEALEDTMAAREVAL 246
gi 154270618  519 hehehELHEGEESEEMKGSTEQ 540
gi 169608982  545 ---peYGRDMLMNAFAVREMGL 563
gi 67528318   516 ----gKGHDCLEDTFAAREVVL 533
gi 85079405   353 ---gkKGHDSVEDAVAARDLIH 371

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