Conserved Protein Domain Family
ExoI_N

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cd06138: ExoI_N 
Click on image for an interactive view with Cn3D
N-terminal DEDDh 3'-5' exonuclease domain of Escherichia coli exonuclease I and similar proteins
This subfamily is composed of the N-terminal domain of Escherichia coli exonuclease I (ExoI) and similar proteins. ExoI is a monomeric enzyme that hydrolyzes single stranded DNA in the 3' to 5' direction. It plays a role in DNA recombination and repair. It primarily functions in repairing frameshift mutations. The N-terminal domain of ExoI is a DEDDh-type DnaQ-like 3'-5 exonuclease containing three conserved sequence motifs termed ExoI, ExoII and ExoIII, with a specific Hx(4)D conserved pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis. The ExoI structure is unique among DnaQ family enzymes in that there is a large distance between the two metal ions required for catalysis and the catalytic histidine is oriented away from the active site.
Statistics
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PSSM-Id: 99841
View PSSM: cd06138
Aligned: 21 rows
Threshold Bit Score: 245.251
Threshold Setting Gi: 91762381
Created: 18-Mar-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1           #### ##                                            ##                        
2QXF_A        12 LFHDYETFGTHPALDRPAQFAAIRTDsEFNVigEPEVFYCKPADDYLPQPGAVLITGITPQEARAk-GENEAAFAARIHS 90
gi 17430149   18 FWHDYETFGAVPRRDRPAQFAGIRTDaELNEigEPVELFCQPSNDWLPDPMSCLITGITPQQCRRq-GIPEHRFAQAIER 96
gi 21242349    6 LFYDLETFGQDPRRTRIAQFAAVRTDaQLRVieEPISFFVQPADDLLPSPYATMVTGITPQHALRe-GVTEAEAFARIAE 84
gi 148927622  10 FFYDLETSGLDPRRDRIMQFAGQRTTmDLDPigEPFNILVRLNDDVLPSPDAVMVTGITPQKTHEe-GIAEAEFIKLFHN 88
gi 151572297   8 LFYDLETSGINNSFDQVLQFAAIRTDlDFNEi-ERFNFFVKLNPDTTPSPMATITHHISIAQANT--GIAEYQAIRKIHK 84
gi 86139894    4 VFYDLETTGISPEFDQPLQFAAIWTDeNFVEk-DRVNIRCRLAPHILPSPQALVVTRVTPDQLIDpsLPSLFEFSQQVAE 82
gi 124008030  24 VFYDTRSSGDNPVFDQILRADTYVTDhAFNLl-ETHHIEIKKRPDVVPSPEALIANNWLPSDLAK--GSTEYEAMRHIHE 100
gi 88800886   12 LWYDYETWGTVPSRDRIAQFAAVRTDmDLNIieEPINLFCKPDIDTVIDPGAVQITRLSPLELEQt-GLSEWEFSQAVHN 90
gi 153872365   6 LFYDIETTGLNKTFAQILQFAAIRTDmQFNEi-ERHDILIKLRPDVIPSPKATITHRISIAQSMQ--GLCEFEAIMQIHR 82
gi 29654660    9 LFYDLETTGLNKCFDQIIQFAAIRTDeALNEl-NRHEILIRLNPDVIPSPEAMIIHRFSIDQMLS--GKAEIEAIREIHQ 85
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                    ##  ###                                                             
2QXF_A        91 LFTV-PKTCILGYNNVRFDDEVTRNIFYRNFYdPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPEND----DGLPSfr 165
gi 17430149   97 ELAT-PGTIGVGYNSIRFDDEITRHLFWRNLIdPYAREWQNTCGRWDLLDVVRTTWALRPDGVEWPKNG----DGKPSfk 171
gi 21242349   85 QMGR-PQTCTLGYNSIRFDDEFIRCGLFRNFYdPYEREWRGGNSRWDLLDVLRLVHALRPDGIVWPQRE----DGATSfk 159
gi 148927622  89 QVCT-PGTVTVGFNNIRFDDEFIRFALWRNFHdPYEWAWKDGRSRWDLLDVVRMTRALRPGGIVWPVDK----SDKPTnr 163
gi 151572297  85 IINA-PGTISIGYNTLGFDDEFLRFAFYKNMLpPYSHQFKNGCSRADLFPIVTCYYLFCNEVLNWPKAIdedgQEKTSlk 163
gi 86139894   83 FTERwAPAIWVGYNTMKFDEEVLRQTFYQNLSpNIYATQFNGNTRFDILPAVYAAYVRHPGLLVWPTDD----TGRRSfk 158
gi 124008030 101 LFNT-PHSYNVGYGNLNNDDLLVRFGFYRNGLtPYTHQWQRSCRRVDLRTIVTLYYLQAPNALVFSKNA----AGKVSle 175
gi 88800886   91 AMAQ-PNTCTTGYNSIRFDDECSRFLFYRNLInPYEREWKNGNSRWDLLDVVRMTHALRPDGIEWPRRD----DGSPSfr 165
gi 153872365  83 LMNQ-SGTISLGYNTSSFDDEFLRFCFHRNLLpPYTHQYKDGCRRSDLLPITIMYYLYKKEVLENWPEI----DGKPTmr 157
gi 29654660   86 LVNT-PGTISVGYNTLGFDDEFLRFSFYRNLLpPYTHQYDNFCGRMDLYPLTILYYLFKPNALNWPDPF----DLKLEn- 159
                        170       180       190
                 ....*....|....*....|....*....|.
Feature 1                        #    #         
2QXF_A       166 LEHLTKANgieh-snaHDAMADVYATIAMAK 195
gi 17430149  172 LEHLSQANglqh-eaaHDALSDVRATIALAR 201
gi 21242349  160 LEHLADANdvre-gdaHEALSDVYATIGMAR 189
gi 148927622 164 LELLTSENnleh-sqaHDALSDVYALIAITR 193
gi 151572297 164 LENLNQNNslysggraHDAITDVIVTVELAK 194
gi 86139894  159 LDRLAPANgfna-hnaHDALGDVEATIHIAR 188
gi 124008030 176 LDDIAQANgltvgkgaTNAQTIMELAKLLHN 206
gi 88800886  166 LEHLTAANglsh-anaHDAVSDVKATIALAQ 195
gi 153872365 158 LEKLSEVNqlai-gqaHNAIVDVEATVELAR 187
gi 29654660  160 LSKANQLAeg----qaHNAMVDVEASVALAK 186

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