Conserved Protein Domain Family
PAN2_exo

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cd06143: PAN2_exo 
DEDDh 3'-5' exonuclease domain of the eukaryotic exoribonuclease PAN2
PAN2 is the catalytic subunit of poly(A) nuclease (PAN), a Pab1p-dependent 3'-5' exoribonuclease which plays an important role in the posttranscriptional maturation of pre-mRNAs. PAN catalyzes the deadenylation of poly(A) tails, which are initially synthesized to default lengths of 70 to 90, to mRNA-specific lengths of 55 to 71. Pab1p and PAN also play a role in the export and decay of mRNA. PAN2 contains a DEDDh-type DnaQ-like 3'-5' exonuclease domain with three conserved sequence motifs termed ExoI, ExoII and ExoIII, with a specific Hx(4)D conserved pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis.
Statistics
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PSSM-Id: 99846
View PSSM: cd06143
Aligned: 23 rows
Threshold Bit Score: 254.463
Threshold Setting Gi: 1730942
Created: 28-Feb-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #### #                                                                       
gi 82231230   970 VGLDAEFVTLNeee-------------------------aeLRSDGTKSTIkp---------sqMSVARITCVRgq---- 1011
gi 146093454  846 IAIDAEYVVLKw----------------------------aSRDEGNEMFYvsqr------kphMGLARVSCILss---- 887
gi 74685515   916 VAIDAEFVALQqee-------------------------meFRSDGTKNILrp---------shMSLARVSVLRge---- 957
gi 1730942    909 VGLDAEFIKIKtd---------------------------lLEFDGKTVQM-------------RAVGRASCVDs----- 943
gi 1175466    887 VGIDSEFVALQqee-------------------------teVRSDGTKSTIkp---------skLSLARVSVLRge---- 928
gi 67609563  1704 VALDSEYVALDveq-------------------------svVRSDGTKEILkk---------sqLSLARVSIVRcgkisl 1749
gi 1709565    907 VAIDAEFVSLQsel-------------------------ceIDHQGIRSIIrp---------krTALARISIIRgee--- 949
gi 156088433   86 VALDVEGVKTGvetlegsryrklpdafssprtmtmnresfvYSADETEEQElstlaryvskhvpRLLNRVSAVRgc---- 161
gi 84995078   939 FALDIEYVKTDlknt-----------------------pvnDSSNEKSSFIvnede----nnylYSLARVTAIRand--- 988
gi 158514332  894 IAIDAEFVVSEpeq-------------------------leIRYTGTRKLIkp---------kkLTLARVSVLRgn---- 935
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                          #   #                                      ## #
gi 82231230  1012 --------gpNEGVPFIDDYIST---QEQVVDYLTQYSGIKPGDLDAKISSKHLTTLKSTYLKLRFLIDVGVKFVGHGLQ 1080
gi 146093454  888 --------kdGDERTIVDDYVHI---PEEIEDYVTQYSGIHPGDLDPLESSKSLTSLKSTYLKLRALVDGGVVFVGHGLA 956
gi 74685515   958 --------geMEGKPFIDDYIHT---SEAVVDYLTEFSGIKAGDLDPNNSPHTLVPLKVAYKKLRLLVDLGCIFVGHGLS 1026
gi 1730942    944 ---------tGERIIFDDHVKLTd--DVEVVDYLTKFSGIVKADLCPTTSEKYLTTHKRLLLRMHVLIQRGVTFVGHALH 1012
gi 1175466    929 --------gpNKGLPFIDDYVAT---DDKVTDYLTEYSGIHPGDLDPDRSPYNVVPLKVAYKKLRLLVNAGCIFVGHGLQ 997
gi 67609563  1750 tgandnveidNKKGLIMDHYVSYgsnSQQPRDYLTKYSGVRPGDLDPKTSSHFLTSKSCILKKLQFLVDAGVVFIGHALP 1829
gi 1709565    950 --------geLYGVPFVDDYVVN---TNHIEDYLTRYSGILPGDLDPEKSTKRLVRRNVVYRKVWLLMQLGCVFVGHGLN 1018
gi 156088433  162 --------geAFGIPFLDHYIHR---REPPKDYLTRFSGIHQGDLDLKSSLHWLTTRKSIYMKIRYLVDAGCKILGHGLE 230
gi 84995078   989 --------dnLHGVPFLDHYILN---NKPPKDYNTRFSGIRSGDLELKSSLHWLTTHKLIYLKLRYLIDNGCKVIGHGLE 1057
gi 158514332  936 --------gpNIGVPFIDDYIVW---TGHIEDYLTSFSGIEPGDLDPEVSKKALVTLQTVYRKLWLLLNMGCVFVGHGLQ 1004
                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
Feature 1         ##                                               #    #         
gi 82231230  1081 KDFRVINLMVPKDQVIDTVYLFHIPRKr-mISLRFLAWYFLDLKIQgETHDSIEDARTALQLYR 1143
gi 146093454  957 QDFRVCNIVVPRKQIIDTLEIFHKPGSr-yLSLRFLAYHVLGESVQeDEHDSIEDARTSLRLYR 1019
gi 74685515  1027 KDFRTINIFVPPEQVMDTVLIYTLPGSqrkLSLRFLAWYLLHQDIQtNSHDSIEDAHFALLLCK 1090
gi 1730942   1013 NDFTVLNVHVAESQIIDTVTLMRLGTQr-mLSLQFLVKEILGETIQmEAHDSVVDARYALKLYR 1075
gi 1175466    998 KDFRIINLLVPPEQVVDTVDLFFLSSRqrkLSLKFLAWYLLDEEIQlTEHDSIEDALTALKLYD 1061
gi 67609563  1830 SDFKIINIYVPPFQIIDTVEIYRLPDEr-yISLKFLAKFVLNKNIQtEVHDSIVDAKTALELFL 1892
gi 1709565   1019 NDFKHININVPRNQIRDTAIYFLQGKRy--LSLRYLAYVLLGMNIQeGNHDSIEDAHTALILYK 1080
gi 156088433  231 QDFKMLNIVVPKSQVIDTVELYRLPGRr-yISLQFLAAHILNIRIQdGEHDSIEDAKAALYLYR 293
gi 84995078  1058 QDLRILNICVPSGCVIDTLELFWIPGKr-yMSLQFLSYHILNQKIQvSEHNSIEDAKTSLHLYK 1120
gi 158514332 1005 NDFRCINLVVPKTQVRDTADLFYLPEFkrkLSLKFLAYVLLKEKVQtGNHDSVEDAYTALMLFQ 1068

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