Conserved Protein Domain Family
REX1_like

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cd06145: REX1_like 
DEDDh 3'-5' exonuclease domain of RNA exonuclease 1, -3 and similar eukaryotic proteins
This subfamily is composed of RNA exonuclease 1 (REX1 or Rex1p), REX3 (or Rex3p), and similar eukaryotic proteins. In yeast, REX1 and REX3 are required for 5S rRNA and MRP (mitochondrial RNA processing) RNA maturation, respectively. They are DEDDh-type DnaQ-like 3'-5' exonucleases containing three conserved sequence motifs termed ExoI, ExoII and ExoIII, with a specific Hx(4)D conserved pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis. REX1 is the major exonuclease responsible for pre-tRNA trail trimming and may also be involved in nuclear CCA turnover. REX proteins function in the processing and maturation of many RNA species, similar to the function of Escherichia coli RNase T.
Statistics
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PSSM-Id: 99848
View PSSM: cd06145
Aligned: 70 rows
Threshold Bit Score: 169.974
Threshold Setting Gi: 159116309
Created: 11-Mar-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1           #### #                                                                       
gi 38604664  145 YALDCEMCYTTHG-----LELTRVTVVDaDMRVVYDTFVKPDNEIVDYNTRFSGVTeaDVAKTSITLPQVQAILLSFFSA 219
gi 124513490 465 YSIDCEMCETTNHq----RELTKITVVDaYMNIIYDSYVIPDNKITNYLTLYSGINesTLENVTTKLKDVQEHLKKFLNK 540
gi 163773539 270 FAVDCEMVRCGSR-----YALARISVVDqDEVTVMDEFVVPDEPVTDYVTRFSGITpeLLANATSRLADIQHRLAQLLRP 344
gi 123478273 194 IAIDCEMVETKLG-----SELARLSVTDfEGKPLLDQLFKPTNEILDYRTPFSGISeeTLANVTVTPDQALKILSKYASR 268
gi 79547863  146 LSIDCEMVTCEDGs----QALVRVGAVDrDLKVVLDKFVKPDKPVIDYKTDITGVTaeDLERATLSVADIQKKLRRFLSV 221
gi 156088805 275 FAIDCEMVTAGGV-----TALARITIVDsLLNTVFDALVKPEGDIQDYRTPYSGITaeSLEDVTIRLSDIQECLNMLIGP 349
gi 115460984 143 LAIDCEMVLCHDGt----EALVRVCVVNsNLEVKLDELVNPLKAVADYRTSITGLSkkDLKGVTCSLVDVQKSLKKLLSK 218
gi 154421722 194 IALDCEMIETTSEdgakhDELARLSVVNeKGEVIIDEYFKPIHPVSDLRTHVSGITqeHLDNAKLTSEDGVSALSAVADK 273
gi 162690144 170 VSLDCEMVTCEGDv----KELVRVCAVGsDYNTLVDELVVPNGKVTDYLTSITGVSekDLQRVTLSQAGVQKLVLDLLTP 245
gi 157345187 125 LAVDCEMVLCEDGt----EGLVRVCVVDrNLQVKLNELVKPHKAVVDYRTEITGVSakDFDETTSSLVDIQRSMKKLLSH 200
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1              ## ###                                                           #    #   
gi 38604664  220 QTILIGHSLESDLLALKLIHSTVVDTAVLFPHYLGFpy--krSLRNLAADYLAQIIQds---------qNGHNSSEDANA 288
gi 124513490 541 KSILIGHSLENDLHALKIAHNYVIDTSIIYCNSGYYpk---pSLFQLSKKHLNIIMKr----------eNGHNSIDDAKI 607
gi 163773539 345 HDILVGHSLENDLGVLQRSHPHVIDTAVLLAREGRYk----qKLSMLTKKHLRYEIQna---------aDGHNSVEDALA 411
gi 123478273 269 KTIIVGHSLENDFRSLKLIHHRCIDTALLYNSETNGvk--kpSLFLLYKKYINKPFRan---------eSGHDSYEDARA 337
gi 79547863  222 GTILVGHGLHNDLQVLRIDHARVIDTSYVFEFVDAPktq-rpSLNNLCKSVLGQEVRmd---------gAAHNCVHDAAA 291
gi 156088805 350 DTILVGHSLDNDLKACEIAHFNVLDTALQYIAPRRHnk---pSLKSLVKQHIGIELVr----------dSGHDSYVDAST 416
gi 115460984 219 GKILIGHSLSKDLHALKFDYSRVIDTAYIFKYANLPtta-saSLNSLCKSVLGYAVRee---------gEPHNCLKDAEA 288
gi 154421722 274 ETIIVGHGLENDFKALLLFHTKVVDTSLIYNNERGVtyprkpKLSNLFQKYFKKEMRdq---------tKPHDSIDDARA 344
gi 162690144 246 GTILVGHSLHYDLRALQIDHKRVIDTSLLFRDPSWPpsy-spSLSNLCQIFDKIIGHqsilkykfreddKPHDCLDDSIV 324
gi 157345187 201 GAVLVGHSLHNDLKALKLDHARVIDTAFIYKYENQPinr-rpSLNNLCKARVGFFLYiiadg--flfhlLGGEYFPFCSV 277

                 ....*.
Feature 1              
gi 38604664  289 CLQLVM 294
gi 124513490 608 SMFLAL 613
gi 163773539 412 CLRLAK 417
gi 123478273 338 AMDLVN 343
gi 79547863  292 AMKLVL 297
gi 156088805 417 TMFLAM 422
gi 115460984 289 AMNLVL 294
gi 154421722 345 ALELSK 350
gi 162690144 325 PMRLVH 330
gi 157345187 278 HFELLF 283

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