Conserved Protein Domain Family
Egl_like_exo

?
cd06148: Egl_like_exo 
DEDDy 3'-5' exonuclease domain of Drosophila Egalitarian (Egl) and similar proteins
The Egalitarian (Egl) protein subfamily is composed of Drosophila Egl and similar proteins. Egl is a component of an mRNA-binding complex which is required for oocyte specification. Egl contains a DEDDy-type DnaQ-like 3'-5' exonuclease domain possessing three conserved sequence motifs termed ExoI, ExoII and ExoIII, with a specific YX(3)D pattern at ExoIII. The motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis. The conservation of this subfamily throughout eukaryotes suggests that its members may be part of ancient RNA processing complexes that are likely to participate in the regulated processing of specific mRNAs. Some members of this subfamily do not have a completely conserved YX(3)D pattern at the ExoIII motif.
Statistics
?
PSSM-Id: 99851
View PSSM: cd06148
Aligned: 16 rows
Threshold Bit Score: 192.114
Threshold Setting Gi: 124800774
Created: 14-Apr-2008
Updated: 17-Jan-2013
Structure
?
Aligned Rows:
 
Feature 1:catalytic site [active site]
Evidence:
  • Comment:The catalytic site is characterized by the presence of four negatively charged residues (DEDD) that serve as ligands for the two metal ions required for activity.
  • Comment:Most DEDDy exonucleases contain a conserved tyrosine at the ExoIII motif which is important in catalysis.
  • Citation:PMID 9396823

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                 # #                                                    
gi 45551159  541 FVTDIILKYaak-----neSIVVSLDCEGINLGLKGEITLIEIGtt-----------rgEAFLFDVQSCpa----mVTDG 600
gi 124800774  55 KECVDEIKNgnynllkdfnMKMIGLDIEGYKIGKYGIVSIIQICy-------------eDIYIFDIYKCdn---vyLFIN 118
gi 66811680  175 NYAIHEIKK----------EKLIGLDIEAIEMGKKGDISLVQIStp-----------ngRIYLFDIIKMgan--vtPFKY 231
gi 124512300  86 KECEEAAEEin-------rSEIIAIDFEGTNLGRYGKVCLMQVYvekknmneqsdnilqKYYIFDLLKT-------SVIK 151
gi 115943126 167 DQAIADMEQ----------QSAIGLVLKGSRLGRKGKLSLVLVLcd------------eQVYMFDVLAVp-----sLFTR 219
gi 158258485  89 GAAILHIKK----------QNVLSVAAEGANVCRHGKLCWLQVAtn------------cRVYLFDIFLLgs----rAFHN 142
gi 147902392 111 GPAIRHLQN----------QKVISIGAVGQNICRHGKLSWLQFAtr------------sRVYLFDVLVLgs----kVFKN 164
gi 116060187 180 KTCVEAMRE----------ADVVAVDCEGVMMSRTGPITVLQCAtr------------dKIYLIDIQALgvkafgaRGSG 237
gi 47230240   18 GAAVIHIKQ----------QCVIGVGAGGLKMSEHGRLCWLQIAtk------------kRVYLFDILLLgs----mAFRN 71
gi 170588753 311 VNAEIDSME----------ERVIGVDFKTVTLGVQGEEFLSLVVlat----------tmQIVVFDVVHSdt----iLLES 366
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                             #                                                          
gi 45551159  601 GLKTVLEH-DQVIKVIHDCRNDAANLYLQFGILLRNVFDTQAAHAILQYQEsg-kqVYKAKYISLNSLCEQYNAPCNp-- 676
gi 124800774 119 YIKDILEC-DDIIKVTHDCREDCSILYNQYNIHLKNILDTQVAYNLLLKNNn---nYTNTYQISYDDLLKKYLFINNn-- 192
gi 66811680  232 GLKEVLES-VKILKVVHDCRRDSEILFHRYQVALAHVYDVQIAHALVQKKIqgnipIRRYGFNELIDLYTSRKYSEIci- 309
gi 124512300 152 SAQKIIEN-KKTLKLIHDCREDSSALYNQLGMKLENVYDTSRAHLLLMEKQk----KNDIYQVSFAQLINDYLGINDa-- 224
gi 115943126 220 KFIDILQA-TNITKVIHDCRFVSDLLYHHYGIELNSVFDTQVGDILIKRRQym--gDFPRNVSGTTQCILEYLEISIhd- 295
gi 158258485 143 GLQMILED-KRILKVIHDCRWLSDCLSHQYGILLNNVFDTQVADVLQFSMEtg--gYLPNCITTLQESLIKHLQVAPky- 218
gi 147902392 165 GLQMVLED-KGILKVIHDCRWLGDILSHQYGIILNNVFDTQVGDVYLFSMEtg--gFLPHGTRTLEECLIHHLSMLPsk- 240
gi 116060187 238 GMRDLLESrEAPLKLMFDCRMDSDALFHQYDVRLENVMDVQILDLATRRALg----LMIDRVAGIAKCTDKHLTEAEtav 313
gi 47230240   72 GISSILES-KEILKVLHDCREIAGFLMGQFGVKLNNVFDTQVADLMCFHSAtg--gFFPDTVSSLEQALSSHLQVPSs-- 146
gi 170588753 367 GVKEILES-EKIIKVIHDAKRVATLLAHRYAINLRKIFDTQVAHAILQYEKfg-kaVSDMRTMSFVNLRRVYYPQSI--- 441
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                          #   #                                         
gi 45551159  677 ----IKDQLKQIYRRDQKFWAKRPLT---REMMLYAAGDVLVLIHDQLFGNLArqik------penrALFSELCTEQILM 743
gi 124800774 193 ----HKIYFHKMITLDNYIYLKRPIM---KELISYAIQDVIYLKPLILCILDKfiik-------qkkKEEQEKNKYVNDK 258
gi 66811680  310 ---dIKFKVKQQFENQPEVWAKRPLP---KDFIDYASLDAACLLPIYYHIKPKlqsnfd---rrflkTHFNEQLTYFKDS 380
gi 124512300 225 ----SLSFIKKEMYKNEKIWETRPLS---NISIIYALKNVKYLYKLYKIFDKLlpkklvl-ekskefVNYCYLNYEYKLP 296
gi 115943126 296 --iaLHLENTQRIEEDESSWFQRPLP---KVNIRCALLDTVYLLRLREAITEQlmae-------ftcAIDIYLTVERDRL 363
gi 158258485 219 --lsFLEKRQKLIQENPEVWFIRPVS---PSLLKILALEATYLLPLRLALLDEmmsd-------lttLVDGYLNTYREGS 286
gi 147902392 241 --vsFLAHRQTLTKEYHDIWFDRPMD---PTLLKLLSLEVTYLMPLRSAMLDAmfsd-------ftlLVDGYLNAYRRGT 308
gi 116060187 314 aadlKVRVRKLYAVEESQLWAERPLT---EDARRYAALDVWLLIKLYDKMKFDl-------------RDDKDDWTSRSLK 377
gi 47230240  147 ----HLLALQTQSQLTQGAFALPPELrelERIPQERREQAINRYPVNEQGLLArfspkaqpgpqtspAAQKPIHTEASSS 222
gi 170588753 442 ----MMSDVTPRKLTHLPSWGLRPIT---EDFLVTVVEEAHCLVTVLYRMISAmlpk-------hllALFEEKCLKALIP 507

                 .
Feature 1         
gi 45551159  744 Q 744
gi 124800774 259 Q 259
gi 66811680  381 I 381
gi 124512300 297 N 297
gi 115943126 364 E 364
gi 158258485 287 A 287
gi 147902392 309 A 309
gi 116060187 378 E 378
gi 47230240  223 S 223
gi 170588753 508 N 508

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap