Conserved Protein Domain Family
S2P-M50_SpoIVFB_CBS

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cd06164: S2P-M50_SpoIVFB_CBS 
Click on image for an interactive view with Cn3D
SpoIVFB Site-2 protease (S2P), a zinc metalloprotease (MEROPS family M50B), regulates intramembrane proteolysis (RIP), and is involved in the pro-sigmaK pathway of bacterial spore formation. In this subgroup, SpoIVFB (sporulation protein, stage IV cell wall formation, F locus, promoter-distal B) contains two tandem repeats of the cystathionine beta-synthase (CBS pair) domain. SpoIVFB is one of 4 proteins involved in endospore formation; the others are SpoIVFA (sporulation protein, stage IV cell wall formation, F locus, promoter-proximal A), BofA (bypass-of-forespore A), and SpoIVB (sporulation protein, stage IV cell wall formation, B locus). SpoIVFB is negatively regulated by SpoIVFA and BofA and activated by SpoIVB. It is thought that SpoIVFB, SpoIVFA, and BofA are located in the mother-cell membrane that surrounds the forespore and that SpoIVB is secreted from the forespore into the space between the two where it activates SpoIVFB. It has been proposed that the CBS domain may play a regulatory role, although its exact function is unknown.
Statistics
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PSSM-Id: 100085
View PSSM: cd06164
Aligned: 58 rows
Threshold Bit Score: 179.657
Threshold Setting Gi: 156743100
Created: 23-Jan-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
active siteputative
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                  ##  #                 
3B4R_A         8 FKIMGIPIELHITFILFLVVIIGLSim------------nnSIFWAVLFILLFVSVVLHELGHSYVAKKYGVkIEKILLL 75
gi 72162214   21 GRPWGIPVYITPSWVVIAVLLTFLYqpvvdsi--lhlgaasYLVALLFAVLLYVSVLIHELAHCVAARHYGLpVRSITLY 98
gi 119960770  21 GRIAGIPVYLAYSWFVIAAFTVIVYgpvlmdym-pylnnwaYFVALCIALLLALSVLIHELAHALSAKAFKWpTEKIVLN 99
gi 117928388  21 GYVFGAPVIVGPSWLLFLAWITWQFapvvadav-pgiregrYLVSACFGVFLGLSVLAHELAHLVAARWFGIpSDRIVLT 99
gi 134098812  20 CRVAGVPVLLSPSWWVGAAVIVLLYtplvgrllpgatsltsGLVAAAFTVLLALSVLLHELGHCLVALRLGLpVRRVRLF 99
gi 84496463   18 ARIGGVPVQIGASWLLLAAVITYIVgsgape-----lgavaYVVGVAYAISLLVSVLVHEGAHAGMARALGFpVHRVVAD 92
gi 119716862  17 GTIAGSDVLVSSSWFLVAGLIAIIVapvvdqaq-pglgawkYVAGVAFAVILYLSVLLHEASHAIAARHFGFpVSSITLH 95
gi 62426229    4 GTVLGAPVILAWSWFLAAIVITILFapwvntvr-pdlgvwaWFVAFAYAVLLFASVFLHELAHGVAGQFYGLkVAAIELN 82
gi 81550102    2 GRPFGVPVYVAPSWFLVAVLITWVFggqldrvl-pelgaarYLVSLFFAVAFYASVLVHELAHTVAALRFKLpVRRIQLQ 80
gi 163840783   2 GRIGGVPIILAYSWFIIAGFTMLVFgpqlseny-ptlgvgaYLVAFVYALLLLFSVLVHELAHALAARAYHWpTQKIVLN 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                    #   
3B4R_A        76 PIGGVAMMDKIP-------KEGELRIGIAGPLVSFIIGIVLLIVSqffdin-----ingypLLYTLSLLNLMLGGFNLIP 143
gi 72162214   99 LLGGVSEIDREAe-----qPRQEFWIAFSGPLLSLVLAGLGFLCYllvdpa-----tvlgvLIWQLWVANLLVGVFNLLP 168
gi 119960770 100 LWGGHTQFENFTa-----sPGRSVVVALAGPASNFLVAGVMWAVMstdlls-----gvsatLGDILMRANLLIGIFNILP 169
gi 117928388 100 ALAGHTALSREPd-----rPRRMFVVALSGPLANLLIAAAAAAAHrvlpah-----tvsavLTAGLAWTNGIVGVYNLLP 169
gi 134098812 100 LLGGITEISRTPp-----rPGQEGVVAVAGPAVSIALAGLTGVAWlalppg-----gavwlLVAQTCVANLAVGVFNLLP 169
gi 84496463   93 LWGGHTAYDARLs-----tPGRAAAIAVVGPVANGALALVAWIASlavsa------dvpsrILFGIAFVNLLLAGFNLLP 161
gi 119716862  96 FLGGMTAIEGEAr-----kPRQEFFIAVVGPLTSIAVGAIALGLRpltpe------glllmAVEGLAGANLLVGVLNLVP 164
gi 62426229   83 IWGGFTRFEPQAdnprdkaALTSFVISIVGPIVNIVLALLGWWCLsavspt-----svpwlLLIAVTFANVALGAINLLP 157
gi 81550102   81 FFGGVSEIEKEAe-----tPGREFVLAFVGPLLSLVLSGVFYAALlpvepd-----svagvLLAGLMVSNLIVAVFNLLP 150
gi 163840783  81 LWGGHTQFENFTa-----tPWRSLVVAFSGPLANFVLAAVGYLVYlslpdgvgtpdrvlnlLLNIFVWANLLIGAFNVLP 155
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #                                                                           
3B4R_A       144 AFPMDGGRILRAILSkky-gylKSTKIAANIGKSLALIMLLFGLls------------------mnIILILVSLFVYFGA 204
gi 72162214  169 GLPLDGGRILRAAVWaasgrplTGTTAAAWGGRILAILVIALPFvyswllwr------aapsivtvLWTTLLGAFIWRNA 242
gi 119960770 170 GMPLDGGRLVESAVWkatgsqdKGTIAAGWSGRIIVVVLVILFIgvplisga-------wpdltfiVITVLVCGFLWMGA 242
gi 117928388 170 GLPLDGGQMLRALVWaitrrprLGVLVGAWSGRLVGLATVLFGAylftrre--------pnaqldgLWALLIGGMLLMSS 241
gi 134098812 170 GLPLDGGRMLRALTWavtgrrhAGTTAGVVGAGAVAVGLIVWALvgllgna--------ddqwlrlGVCVLLAWFVVAGA 241
gi 84496463  162 GLPLDGGQLVDALVWkltgrreKGLIAAGWTGRVVTLGVIGWFVvrplvegs-------qpsltsiIWMGLICVFMWQGA 234
gi 119716862 165 GLPLDGGRVLKSLVWrltgsphRGTIVAGWGGRLTAAAVLFWPAfqepvlgt-------epdildfVLAFVIAAFLWSGS 237
gi 62426229  158 GIPLDGGWALQALMWrltgsqyLGTIVASWVGRVIAIGFIGWSVitpllage-------rpdpltvAWMSLIAIMLWFSA 230
gi 81550102  151 GLPLDGGRMLRAVVWkltgkpmSGTIAAAWVGRALAIAVLIGLPlltqslgsdaaddvgmdtvmdaLLAAILAAIIWTGA 230
gi 163840783 156 GLPLDGGRLVESIVWritgsqeKGTVAAGWAGRVVVVLLVFTVLgvpllvrq-------plniqtaLFSLFIAAFLWVGA 228

                 ....*..
Feature 1               
3B4R_A       205 EQESRVV 211
gi 72162214  243 SVALHTA 249
gi 119960770 243 SSSIHHA 249
gi 117928388 242 GAVLRQQ 248
gi 134098812 242 GAEHNAE 248
gi 84496463  235 TGAIRTG 241
gi 119716862 238 TAAMASA 244
gi 62426229  231 GDAATHA 237
gi 81550102  231 GNSLRMA 237
gi 163840783 229 SQAITTA 235

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