4D70


Conserved Protein Domain Family
Sortase_D_2

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cd06166: Sortase_D_2 
Click on image for an interactive view with Cn3D
Sortase domain found in subfamily 2 of the class D family of sortases
Class D sortases are cysteine transpeptidases distributed in Gram-positive bacteria (mainly present in Firmicutes). They anchor surface proteins bearing a cell wall sorting signal to peptidoglycans of the bacterial cell wall envelope, which is responsible for spore formation under anaerobic conditions. This involves a transpeptidation reaction in which the surface protein substrate is cleaved at the cell wall sorting signal and covalently linked to peptidoglycan for display on the bacterial surface. The prototypical subfamily 2 of class D sortase from Clostridium perfringens (named Cp-SrtD) recognizes the LPQTGS signal motif for transpeptidation. Its catalytic activity is in a metal cation- and temperature- dependent manner. The presence of magnesium appears to enhance Cp-SrtD catalysis towards the LPQTGS signal motif.
Statistics
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PSSM-Id: 320681
View PSSM: cd06166
Aligned: 121 rows
Threshold Bit Score: 120.661
Threshold Setting Gi: 917478082
Created: 14-May-2008
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
catalytic site
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                           #                            
4D70_A        58 GDGIALIDIEKIGVHTVIAEGSTLd-vLENNIGHFENTAMPGenGNFSIAGHRNtinnEVFRNIDKLqvGDEIKITTl-T 135
gi 941191636  88 EGCIGIVTVPSVGIQMQVYETDDAmedMKHGVAHFKSTSAWE--GNIGLSGHNYr---SQFGPLTRVkdGDIIAYETa-L 161
gi 933749725 245 SGSIGTISIPSINLKNAPVMGSVElsdLAKGTGHFENTPILD--GNVGIAGHNN----THFKYLANVdiGDEINYTVn-G 317
gi 524743353  90 QQIIGKITCDKIGIEYIVVEGADRd-nIRATIGHIKGTAGLGgkGNCVLAGHRGgyygTFFKNIDQLtkGDVVVVTDlyN 168
gi 291526663  72 GGVIGIIEIPGLNIRYPVMEGTASk-vLNAGIGHIEETAGIGesGNCVLCGHNGsrygTFFTPLSQIsiGDEIMITDknG 150
gi 507764662  82 GNVIGLIEIEALDLKYPIVEGADSk-qLAYGIGHISDTAAVGsmGNCVLAGHRGsrygTYFKYLNRLseGDTVKVMDkaG 160
gi 511031790  64 EGAAGMVEIPELKIKYPVFEGTGTv-qLNEGIGHMESTAPLCgeGNCVLAGHNGsrrgVFFTNLSSAkaGTEVKLTTkaG 142
gi 652830954  63 KDAIGIIEIDSLDIHYPIYEGATEa-qLSRGIGHLPETAGLLeqGNCVLCGHNGssrgLFFTTLSHIrkDAQVRITTkdK 141
gi 524321471  96 LTLIGAIKLPKIGVSENVVEGTGAe--LYYAAGHMRGTAEIGqeGNCVIAGHRDyvkmNPFSYLNKMeiGDDIILEDp-N 172
gi 933756475  94 LTVVGYFKFPKLGVSENIVLGSDEe--LMYGVGHVNGTAGLGqeGNCVLAGHRNyivmHPFRHLDKAavGDRVMVGDe-S 170
                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
Feature 1                                           #        #      
4D70_A       136 DIFQYEINEIFVTsPSDTDVLNQNl-dEKTMTIVTCTn--rGKDRYIVKAK 183
gi 941191636 162 GTRYYQVSAIVEIaDDDWSYLDRTd--DNRITLITCIvg-eDDKRLVVQGT 209
gi 933749725 318 VTRTYKVTDMRAIsDMDWGVFTDYg--DNRLTLITCEha-vPNRRIAVTAI 365
gi 524743353 169 RNYYYEVYEQQVVeADALWICEPVe-gKNTLTLLSCEd--hGTKRRIVRCQ 216
gi 291526663 151 LKHIYEVTETEIVnPYDNSIKTQGd--EKELTLFTCSq--kGTMRFVVRCI 197
gi 507764662 161 NVYLYEVVSWEVVgPYDNSVKAQGe--ETELTLLTCEn--sGTMRLIYHCI 207
gi 511031790 143 VTHCYTVEEMRVVnPYDGWVTEECd--TEVLTLFTCAe--hGTKRFACRCV 189
gi 652830954 142 RSRIYCVKSTRVVsPHDPSVRKKRd--EEVLTLFTCAy--hGTRRFVATCV 188
gi 524321471 173 YRYIYKVYRIETVlPSDTWVTEKQedkEKICTLITCTpritSTHRLIVFGE 223
gi 933756475 171 NVYTYEIYEILTVkPEDVYVTELQegeSHMLTLVTCTpvlnPTHRLVAWAR 221

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