Conserved Protein Domain Family
CYPOR_like

?
cd06182: CYPOR_like 
Click on image for an interactive view with Cn3D
NADPH cytochrome p450 reductase (CYPOR) serves as an electron donor in several oxygenase systems and is a component of nitric oxide synthases and methionine synthase reductases. CYPOR transfers two electrons from NADPH to the heme of cytochrome p450 via FAD and FMN. CYPOR has a C-terminal ferredoxin reducatase (FNR)- like FAD and NAD binding module, an FMN-binding domain, and an additional conecting domain (inserted within the FAD binding region) that orients the FNR and FMN binding domains. Ferredoxin-NADP+ (oxido)reductase is an FAD-containing enzyme that catalyzes the reversible electron transfer between NADP(H) and electron carrier proteins such as ferredoxin and flavodoxin. Isoforms of these flavoproteins (i.e. having a non-covalently bound FAD as a prosthetic group) are present in chloroplasts, mitochondria, and bacteria and participate in a wide variety of redox metabolic pathways. The C-terminal domain contains most of the NADP(H) binding residues and the N-terminal domain interacts non-covalently with the isoalloxazine rings of the flavin molecule which lies largely in a large gap betweed the two domains. Ferredoxin-NADP+ reductase first accepts one electron from reduced ferredoxin to form a flavin semiquinone intermediate. The enzyme then accepts a second electron to form FADH2, which then transfers two electrons and a proton to NADP+ to form NADPH.
Statistics
?
PSSM-Id: 99779
View PSSM: cd06182
Aligned: 15 rows
Threshold Bit Score: 241.087
Threshold Setting Gi: 13518089
Created: 12-May-2008
Updated: 17-Jan-2013
Structure
?
Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 11 residues -Click on image for an interactive view with Cn3D
Feature 1:FAD binding pocket [chemical binding site]
Evidence:

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                    #    
1AMO_A        222 AVTANRKLNQgte----------------------rhLMHLELDisd-------------------skIRYESGDHVAVY 260
1DDG_A         15 SLSVNQKITGrns---------------------ekdVRHIEIDlgd-------------------sgLRYQPGDALGVW 54
1GAQ_A         41 RCLLNTKITGdda---------------------pgeTWHMVFSte--------------------gkIPYREGQSIGVI 79
1TLL_A        255 RLLSRQNLQSpks---------------------srsTIFVRLHtng------------------nqeLQYQPGDHLGVF 295
2QTL_A        118 PISKAVQLTTnda---------------------iktTLLVELDisn-------------------tdFSYQPGDAFSVI 157
gi 117298     666 NVVASKELQQpgs---------------------arsTRHLEIElp--------------------keASYQEGDHLGVI 704
gi 2981043    511 WSLARRECLNpgsq--------------------gesTWLLDLRapa------------------nsaIQWRAGDLLEIV 552
gi 13518089   286 SSASSTSTTPspd---------------------pekVRLLEACassetgpqll-----rslsksstpLCYPSLVDVLEV 339
gi 116055646  674 AITGNGARMN---------------------------TVWLKFRliha----------------reevQYYKPGDHVAVW 710
gi 148547954  451 LETVNRQCPQafarwghalgdvlgqalalvhtpqqprTHSLELTeriaygeqvnapthvlrfnargglPNFLAGDLVGIL 530
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
1AMO_A        261 PANdsalvnqigeilgadldvimslnnldeesnkkhpfpcpttyrtaltyylditnpprtnvlye------laqyaseps 334
1DDG_A         55 YQNdpalvkelvellwlkgdepvtvegktlplnealqwhfeltvntaniveny--------------------------- 107
1GAQ_A         80 ADGvdk-------------------------------------------------------------------------- 85
1TLL_A        296 PGNhedlvnalierledappanhvvkvemleerntalgvisnwkdesrlppctifqafkyyldittpptplqlqqfasla 375
2QTL_A        158 CPNsdsevqsllqrlqledkrehxvllkikadtkkkgatlpqhipagxslqfiftwcleiraipkka--flralvdytsd 235
gi 117298     705 PRNyegivnrvtarfgldasqqirleaeeeklahlplaktvsveellqyvelqdpvtrtql-------------ramaak 771
gi 2981043    553 PHQapvrirewlqrhnldgqarvavegveqsl------------------------------------------------ 584
gi 13518089   340 FSFvqipldr---------------------------------------------------------------------- 349
gi 116055646  711 PQTseararyfaahfgltfrdvlelvpksdallvkksidpaipnvvsvehlftrvldingeasa--------allsalah 782
gi 148547954  531 PPG----------------------------------------------------------------------------- 533
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                 ####                 # #
1AMO_A        335 eqehlhkmasssgegkelylswvvearrhilailqdypslrppidhlcellpRLQARYYSIASSSkvh---pnsVHICAV 411
1DDG_A        108 ---atltrsetllplvgdkaklqhyaattpivdmvrfspaqldaealinllrPLTPRLYSIASSQaev---eneVHVTVG 181
1GAQ_A         86 -------------------------------------------------ngkPHKVRLYSIASSAigdfgdsktVSLCVK 116
1TLL_A        376 tnekekqrllvlskglqeyeewkwgknptmvevleefpsiqmpatllltqlsLLQPRYYSISSSPdmy---pdeVHLTVA 452
2QTL_A        236 saekrrlqelcskqgaadysrfvrdaxaclldlllafpscqpplslllehlpKLQPRPYSCASSSlfh---pgkLHFVFN 312
gi 117298     772 tvcpphkveleallekqaykeqvlakrltmlellekypacemkfsefiallpSIRPRYYSISSSPrvd---ekqASITVS 848
gi 2981043    585 -----------------------eqalagrllpdsfehlvglhpqalldaliPLSVRQYSIASLQsd-----gdLQLIVR 636
gi 13518089   350 ----------------------------------------------llevsgPLQTRRYSLANWIpatl-ppspLQLCMR 382
gi 116055646  783 yafnedarqdwaemtgarifsifehfptlstlhrgdkavgldmlrdiilkipKLRPRYYSVSSSPqhag--nnvFALTVG 860
gi 148547954  534 ----------------------------------------------------SPIPRFYSLASGSqd-----gvLEICVR 556
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                    ####                                 
1AMO_A        412 AVEYeaksg------------------------------rvnkGVATSWLRAKepag----------------------- 438
1DDG_A        182 VVRYdveg-------------------------------raraGGASSFLADRve------------------------- 205
1GAQ_A        117 RLIYtndag------------------------------eivkGVCSNFLCDLq-------------------------- 140
1TLL_A        453 IVSYhtrdge----------------------------gpvhhGVCSSWLNRIq-------------------------- 478
2QTL_A        313 IVEFlstatt----------------------------evlrkGVCTGWLALLvasvlqpnih------------ashed 352
gi 117298     849 VVSGeawsg-----------------------------ygeykGIASNYLAELq-------------------------- 873
gi 2981043    637 QEQHadg----------------------------------slGICSGWLTEYlp------------------------- 657
gi 13518089   383 EVCArrsanlpaatavgadaqrvadmlnraaqdasrdhsdfffGHTSHPLCCAarsmtrsaraagqrgmyvsfslfgnsl 462
gi 116055646  861 RVTYksgag-----------------------------armhlGFCSDFLATLp-------------------------- 885
gi 148547954  557 KHSG---------------------------------------GMCSEFLHGLd-------------------------- 571
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
1AMO_A        439 -enggRALVPMFVRKs-QFRLPfk-stTPVIMVGPGTGIAPFMGFIQERawlr---eqgkEVGETLLYYGCRRsdEDYLY 512
1DDG_A        206 ----eEGEVRVFIEHndNFRLPan-peTPVIMIGPGTGIAPFRAFMQQRaa-------deAPGKNWLFFGNPHftEDFLY 273
1GAQ_A        141 ----pGDNVQITGPVgkEMLMPkd-pnATIIMLATGTGIAPFRSFLWKMffekh--ddykFNGLGWLFLGVPTs-SSLLY 212
1TLL_A        479 ----aDDVVPCFVRGapSFHLPrn-pqVPCILVGPGTGIAPFRSFWQQRqfdiq--hkgmNPCPMVLVFGCRQskIDHIY 551
2QTL_A        353 sgkalAPKISIFPRTtnSFHLPdd-psIPIIMVGPGTGIAPFIGFLQHReklqe-qhpdgNFGAMWLFFGCRHkdRDYLF 430
gi 117298     874 ----eGDTITCFISTpqSEFTLpkdpeTPLIMVGPGTGVAPFRGFVQARkqlk---eqgqSLGEAHLYFGCRSphEDYLY 946
gi 2981043    658 ----lGAALTLRLRRnaGFHLPed--dVPLILIGNGTGLAGLRSLLRASia--------aGRKRNWLLFGERNfaHDYYC 723
gi 13518089   463 farqlQAGCTALCNPaqAKSLC-----SQLFLIGCGTGIAPLIAAVTQLmlrrastaagsAPFPCWVFYGARTk-AELLY 536
gi 116055646  886 ----iGANVTVEFRPapSFRLPrs-pqAPIIMVAGGTGIAPFKGFVEHFatl-----npgERGEAWLIAGCRSq-DNQLY 954
gi 148547954  572 ----iGAQIDAFIQPnpQFRPAsg--tHPVILIGAGTGIGPLAGFIRNNk----------ARHPMHLYWGGRNpaSDFLY 635
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
1AMO_A        513 REELARfhk-dgaLTQLNVAFSREQah------kVYVQHLLKrd-rEHLWKLIHEGgaHIYVCGDAr---nMAKDVQNTF 581
1DDG_A        274 QVEWQRyvk-egvLTRIDLAWSRDQke------kVYVQDKLReq-gAELWRWINDGa-HIYVCGDAn---rMAKDVEQAL 341
1GAQ_A        213 KEEFGKmkerapeNFRVDYAVSREQtnaa--gerMYIQTRMAey-kEELWELLKKDntYVYMCGLKg----MEKGIDDIM 285
1TLL_A        552 REETLQakn-kgvFRELYTAYSREPdr-----pkKYVQDVLQeqlaESVYRALKEQggHIYVCGDVt----MAADVLKAI 621
2QTL_A        431 RKELRHflk-hgiLTHLKVSFSRDApvgeeeapaKYVQDNIQlh-gQQVARILLQEngHIYVCGDAk---nMAKDVHDAL 505
gi 117298     947 QEELENaqs--egIITLHTAFSRMPnq-----pkTYVQHVMEqd-gKKLIELLDQGa-HFYICGDGs---qMAPAVEATL 1014
gi 2981043    724 RAELEQala-rgeLQRLDLAFSRDQae------kIYVQDRLHea-aDELRRWIDEGa-ALYVCGSLq---gMAGGVDRVL 791
gi 13518089   537 DETLQEalr-tgaIAKYEYALSREEdnk---kqgRYVTDLVKrn-rLMVTGSLQNEg-QLFVCGPAkallsVRQLVKCDL 610
gi 116055646  955 ATEFNEavh-lghLTKYLVGFSRQPgv-----pkTYVDTVLReh-aDDLMDLISRGa-HVYVCGDVrietsVRGAMLDIL 1026
gi 148547954  636 EPELNQyls-drrLTQLHAAFSQVQe-------rSYVQDRLIsd-aLALRRLIEKGa-QVLVCGSRe----MAKGVMQAL 701
                         490       500       510
                  ....*....|....*....|....*....|....*
Feature 1                                            
1AMO_A        582 ydivaefgpmehtqaVDYVKKLmt--kgRYSLDVW 614
1DDG_A        342 leviaefggmdteaaDEFLSELrv--erRYQRDVY 374
1GAQ_A        286 vslaek----dgidwFDYKKQLkr--gdQWNVEVY 314
1TLL_A        622 qrimtqqgklseedaGVFISRLrd--dnRYHEDIF 654
2QTL_A        506 vqiiskevgvekleaMKTLATLke--ekRYLQDIW 538
gi 117298    1015 mksyadvhqvseadaRLWLQQLee--kgRYAKDVW 1047
gi 2981043    792 rev----------lgEEALQRLad--egRYRRDVY 814
gi 13518089   611 laepdd----ddsvqEQRLLMLed--rgRLNFDIW 639
gi 116055646 1027 g-------------gEAAFNELek---sRYHLDIF 1045
gi 148547954  702 dev----------laPLHLSVLilkaqgRYREDVY 726

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap