Conserved Protein Domain Family
cyt_b5_reduct_like

?
cd06183: cyt_b5_reduct_like 
Click on image for an interactive view with Cn3D
Cytochrome b5 reductase catalyzes the reduction of 2 molecules of cytochrome b5 using NADH as an electron donor. Like ferredoxin reductases, these proteins have an N-terminal FAD binding subdomain and a C-terminal NADH binding subdomain, separated by a cleft, which accepts FAD. The NADH-binding moiety interacts with part of the FAD and resembles a Rossmann fold. However, NAD is bound differently than in canonical Rossmann fold proteins. Nitrate reductases, flavoproteins similar to pyridine nucleotide cytochrome reductases, catalyze the reduction of nitrate to nitrite. The enzyme can be divided into three functional fragments that bind the cofactors molybdopterin, heme-iron, and FAD/NADH.
Statistics
?
PSSM-Id: 99780
View PSSM: cd06183
Aligned: 142 rows
Threshold Bit Score: 114.586
Threshold Setting Gi: 46095632
Created: 31-Jul-2008
Updated: 17-Jan-2013
Structure
?
Program:
Drawing:
Aligned Rows:
 
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:FAD binding pocket [chemical binding site]
Evidence:
  • Structure:IB0: Rat cytochrome b5 reductase binds FAD, contacts determined at 4A
    View structure with Cn3D
  • Structure:1CNF: Zea mays Cytochrome B Reductase binds FAD, contacts determined at 3.5A
    View structure with Cn3D
  • Citation:PMID 7812715
  • Comment:FAD contacts primarily in the N-terminal region
  • Citation:PMID 7893687

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                        #              ##
1IB0_A         18 LRLIDKEILs---------------------hDTRRFRFALPsp---------qhILGLPIGQHIYLStrid-gnlVIRP 66
gi 116501623   77 ATVVSNLDSg---------------------pDTKLLRLALPpqvaa---faaqdPSSFKAVWSVFIKddd---iqVERP 129
gi 89309503     5 TTFVSVSTYyqnnl--------------nelqNEIFLKTVLDnp---------dqHLGTKVGQAVKLTlnsqkqnqISRY 61
gi 46095632   126 FPLISSSYYpepd----------------rasVHKLLHIGVPasvvgsdvrnedkLKQLLRVRSIYIKeps---lvIERA 186
gi 156843148   64 YRVSYKKPI-----------------------DGSHFLMELTpvvkqr--tnlwlKLESEKLWSVEVKqpe---imVARN 115
gi 58258325    81 HKLVTIKIPsss-----------------ygfFDKPYRLDGEva---------dvKGGEVVIQHVMVKspd---iqIERP 131
gi 2330719     45 FTVNKITELt---------------------sDASLFSLVPQsps--------ehLTTLEPIAKVTIRdps---mqVQRP 92
gi 146100590  942 VPVVVRESRasdq----------------fgvGSRVLRFNLPga---------tqTSGLTVGEFIGIRgdwd-gqqLIGY 995
gi 146177838   37 QFLIKKQISp----------------------TSSIYRFKMNde---------rkVLGIQLGENIQIGvpgnqqenHIAK 85
gi 60460036   963 ATILQKREIalsktswtsvvvresrsgeqfgtGSRVLRFNLPga---------lqRTGLNLGEFVAIRgewd-gqqLVGY 1032
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         ##                            ### #          #####                              
1IB0_A         67 YTPVSSDDDKg---------------fVDLVVKVYfkethpkfpaGGKMSQYLENMn---IGDTIEFRGPn--------- 119
gi 116501623  130 YTPLEGIDENg---------------eMVFWIKKYp---------RGEVGRWLHTKa---AGDVIELRGPl--------- 173
gi 89309503    62 YSLVSDPFSKg---------------yFDCIVKDLdksn--yaenSNPMVPQLLQLk---DGAKVVIEDPy--------- 112
gi 46095632   187 YTPLYDTLPGsysmalvg-asaeekqrVDLLVKRYp---------DGELGKMLHRAvpnpAVPQIEVRGPvdt-----ws 251
gi 156843148  116 YTPLPLKYDAqsddivplkdgdnaggkLFFYLKQYp---------DGEVSRWLFKMk---EGDQVELRGPi--------- 174
gi 58258325   132 YTLINDPNAAnge------------reMRMVVKRVr---------GGEVGRVVHTTk---EGEMLGVRGPi--------- 178
gi 2330719     93 YTPLYLDANE-----------------LKFFIRKYe---------EGPVSSYIHSKk---EGDTIELRGP---------- 133
gi 146100590  996 YSPINMPDDKg---------------rISILARGD----------KGNLQEWISSMr---PGDSVEMKACg--------g 1039
gi 146177838   86 VHPLTRIDDPa---------------iVDVLVDEQ----------IPEQQFFSNLK----EKQEVVIKSDq--------n 128
gi 60460036  1033 FSPITLPEDLg---------------tISLLVRAD----------KGTLKEWICALr---PGDSVEIKACgglridqdpv 1084
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                       #  ##                             
1IB0_A        120 GLLVYQGKGkfairadkk-----------snpvvrtvKSVGMIAGGTGITPMLQVIRAVLkdp-----ndhTVCYLLFAN 183
gi 116501623  174 TTWPWKDDTw---------------------------DEVVMISGGTGFTPFYQLFHSIIsnps---lspsTQFTFLHSS 223
gi 89309503   113 TQYIYHGLGnltfkkkqivqs-----gfnsefktgrfDKIGIIAHNSAITTFSQLIQGVIsar------dpTQINLLFHN 181
gi 46095632   252 FERDSAASGei-------------------------pDRIVMIVGGTGITPAYQLLTNLFgrpctsatstaDKLSVSLFS 306
gi 156843148  175 TEYELPRDSderkrdrgflfdsktiknhdvetfrwrpFDISFYAAGTGIAPALQLTLSEDpf--------kGKLQLFYSC 246
gi 58258325   179 PTFSIYPQRy---------------------------DKIIMISTGTAVSPFLQLLAKLSps-------naPRLELIQAL 224
gi 2330719    134 FKTTKLDCTk--------------------------yPRIVAIVAGTGIAPIYQLAQSVK-----------SPVDIVYCS 176
gi 146100590 1040 LRIELKPHQkqmvy------------------rktviRKLGLIAGGSGVAPMLQIIKAALnrpy---vdsiETIRLVYAA 1098
gi 146177838  129 KKFVYDGFGkikifnqg-------------qvtqkkvDYLGIIVQGYHISKVFSLIEGIStng------dkTNISILYVN 189
gi 60460036  1085 KKCLLFRNRp--------------------------iTRFALVAAGTGVAPMLQVIRAALkkpy---vdtlESIRLIYAA 1135
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
1IB0_A        184 QSEkd----------iLLRPELEELRneh----------------ssRFKLWYTVDKapdaw-----------dysqGFV 226
gi 116501623  224 RTPeelp------pasLLNPLITFAQen-----------------paRLKVNLFVDQneskss--------gydlsvTRI 272
gi 89309503   182 HKIqd----------eILLDEMTRFFls------------------hNMKYSTVLQNiiy--------------qpsKFI 219
gi 46095632   307 EKLagspsalspaelsALGPQLTASSsgrswlsslw--gksrsaldpQLRLTSSQSPttti------------pvyeSRI 372
gi 156843148  247 KQLee---------lgPLAKYLELLQqn------------------gRIELNLYESSkgnslrkdtkklldtipapsKYV 299
gi 58258325   225 PALsss-------tppFSHESLDRAApikwdwvdtnedpsflpslekKFGEKLKITRfpqg------------avpaGAV 285
gi 2330719    177 RPGqp----------pLLKEELEKECp--------------------NVRVKSVQNRlvn---------------ihDIL 211
gi 146100590 1099 EDEye----------lTYRSLLKQYRadn----------------pdKFDCGFVLNNppegw-----------tegvGYV 1141
gi 146177838  190 SNLde----------sLFIDELTWYAe--------------------EKKIHLGVLFekvpdgvp----vmkgkfqkHHV 235
gi 60460036  1136 EEYdt----------lTYRSILQRFAeef----------------pdKFVCNFVLNNppegw-----------tggvGFV 1178
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
1IB0_A        227 NEEMIrdhlpp-----------------pgeetlILMCGPPPMIq-------------fACLPNLERvgh---------- 266
gi 116501623  273 NADVIkrsigldkegkswwnwlygkkpeapkrrlFLVCGPDPMVaaiagpf-grnmsqgQLGGALSKlgf---------- 341
gi 89309503   220 QKKAKsdyygfngdvcers--iiqtlpsprenyvIMLQGPRPFKe--------------KVYQLLLKmg----------- 272
gi 46095632   373 VQQHIeqllspikp------------qqktgrtlILVSGPDGMVeaiagp----ksrdgQHQGSLSVssklsvytpaffr 436
gi 156843148  300 PKATFksnsnas----------------iispvlSLVCGPDEYIstiagpk--ylqaqgQTGGLLKQkgw---------- 351
gi 58258325   286 QAALEgmnr---------------------ervlVLVCLPPWLMrplcggm-trnldqgPVTGVLRElgl---------- 333
gi 2330719    212 DWDNVtvpl---------------------kdtlCIVCGSQKFVstiagpkadygarqgEVKGLLSNnp----------- 259
gi 146100590 1142 DRATLqsllpp-----------------pskgllVAICGPPVMQr--------------SVVADLLAlgy---------- 1180
gi 146177838  236 SDFMPpvdq----------------------efhLIVAGSNTFQd--------------ECLGHLKSlgy---------- 269
gi 60460036  1179 NKKSLqkvlqp-----------------psseplIVVCGPPVMQr--------------DVKNELLSmgy---------- 1217

                  ....*...
Feature 1                #
1IB0_A        267 pkERCFTF 274
gi 116501623  342 ksQDVYKL 349
gi 89309503   273 ydSERIAY 280
gi 46095632   437 lpSRRTLF 444
gi 156843148  352 neENVYKL 359
gi 58258325   334 gsKQVYKL 341
gi 2330719    260 -fGKVWKL 266
gi 146100590 1181 naEMVRTV 1188
gi 146177838  270 knENITLY 277
gi 60460036  1218 dkELVHTV 1225

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap