Conserved Protein Domain Family
flavohem_like_fad_nad_binding

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cd06184: flavohem_like_fad_nad_binding 
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FAD_NAD(P)H binding domain of flavohemoglobin. Flavohemoglobins have a globin domain containing a B-type heme fused with a ferredoxin reductase-like FAD/NAD-binding domain. Flavohemoglobins detoxify nitric oxide (NO) via an NO dioxygenase reaction. The hemoglobin domain adopts a globin fold with an embedded heme molecule. Flavohemoglobins also have a C-terminal reductase domain with bindiing sites for FAD and NAD(P)H. This domain catalyzes the conversion of NO + O2 + NAD(P)H to NO3- + NAD(P)+. Instead of the oxygen transport function of hemoglobins, flavohemoglobins seem to act in NO dioxygenation and NO signalling.
Statistics
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PSSM-Id: 99781
View PSSM: cd06184
Aligned: 129 rows
Threshold Bit Score: 222.433
Threshold Setting Gi: 172041370
Created: 31-Jul-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 13 residues -Click on image for an interactive view with Cn3D
Feature 1:FAD binding pocket [chemical binding site]
Evidence:
  • Structure:1GVH: E. coli flavohemoglobin binds FAD, contacts determined at 3.5A
    View structure with Cn3D
  • Structure:1CQX: Cupriavidus necator flavohemoglobin binds FAD, contacts determined at 3.5A
    View structure with Cn3D
  • Comment:FAD contacts are primarily in the N-terminal sub-domain

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                   #               ####             # # 
1GVH_A       147 GGWEGTRDfr--iVAKTPRSalITSFELEPVDGgavaEYRPGQYLGVWLkpegfphqeIRQYSLTRKPDgk-gYRIAVKR 223
gi 158424375  32 SGWRAFTV-----AERHPETaqITSFHLVPADGgappVHKAGQHLTLRLsdp---srlIRNYTISTAPNgh-hWRISVKR 102
gi 23006958  152 GGWNGWRDfv--vESVTPESeiIRSFVLVPADGgpvlRHEPGQYLGFLLdlpg-rgvlKRNYSISCAPNdr-aYRITVKR 227
gi 149909197   3 ETFKSFTI-----LKKHKEStlVTSFHLTPTDGsslwDAEPGQYLTLRIptgt--gfvLRSYSISGDVEnlqsYRISVKR 75
gi 162146318 155 GGWTGWRPft--vRRRTRESetVTSFELVPVDGapvmRHRPGQYLSFRLdvpg-lgsqRRNYSISSKPDdg-sYRISVRR 230
gi 90023074  327 RVYKQFKI-----IKKIKESrdVTSFYIRPLDGsalsSYLPGQFLPISIkqphtsstcLRTYTLSQAPLsn-aYRFSVKR 400
gi 161383291 152 GGWKGWRDfv--iAGIIGESasVKSFVLRPVDGgpvlRHQPGQYLAFDFdhpd-tgkaRRNYSISCAPNge-yYRISVKR 227
gi 58039327  155 GGWTGWRPfr--iARAVPECanVTSLELEPVDGkpviSAVPGQYLGVDLdipd-rgrtRRNYSITSSPNrk-gYRISVRH 230
gi 150017144  11 NKWKGFKDflvykTVKEDEV--VTSFYLKSLDGsklpEFIAGQFITVRIknedntftkPRQYTLSMNSNee-fYRISVKR 87
gi 92112585  149 GGWEGTRTfr--iAAKRPESavITSFVLAPVDGgpvaAHRPGQYIGFKLtidg--eivHRHYSLSASPDgk-tYRVSIKR 223
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                    ####                                         #                      
1GVH_A       224 Ee---------gGQVSNWLHnhANVGDVVKLVAPAGDFFMavaddTPVTLISAGVGQTPMLAMLDTLaka----ghtAQV 290
gi 158424375 103 E-----------GAASTWLHdaLRVGDTLEVAGPGGDFVLpssaeRPLVLVSAGVGVTPMVAMLEEAvaq----gwsGPL 167
gi 23006958  228 Eakte----hpaGLVSNWLHdaAIAGTVLKVAAPAGDFFLdrssnAPVVLVSGGVGLTPMVSMLESItet----tpeRPT 299
gi 149909197  76 EtanvdwegaldGIGSCWLHdlAKIGDEIDISAPRGQFVLdnnsvRPIVLLSAGVGLTPLVSMLHSLkk------reNDV 149
gi 162146318 231 Ip---------gGAVSGWLHddVREGTVLQVSAPAGDFTLadplpPAIVLLSAGVGLTPMISMLDALaar----adaPSI 297
gi 90023074  401 Ek---------fGLASRILHdtINTGDSLYVSQPEGKFTLqt-nkKTNVLLSAGIGITPMIAMLQGLirevekgekpQDV 470
gi 161383291 228 Ep---------gGVISGWLHevATEGTVLRVAAPAGDFVLkdrpeGEVVLLSAGVGLTPMVAMLETLaa------ndRSA 292
gi 58039327  231 Vs---------nGAVSGWLNsgKCEGMEVLLSPPAGNFVLpekveGPLAFVCAGIGITPMIGMLEALaqp----gvtTPV 297
gi 150017144  88 Ee---------nGYLSKKLCdeIKAGDNLQITAPLGNFILkd-seKPLVLIGGGIGITPMLTMAYDAvn------sdRKI 151
gi 92112585  224 Ee---------gGKVSRHFHdrLAEGDTLELLPPAGHLTLde-gtSPVMLISAGVGQTPMVPLARQAla------agRRV 287
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1GVH_A       291 NWFHAAENGdvHAFADEVKELGqs--------lpRFTAHTWYRQpseadra-kgqfdseGLMDLskleg------afsdp 355
gi 158424375 168 HFIHCTRDAahQAFAEHVADLLak--------lpAGRSDIFYSRapqagl---ganqhgGRVTLdflrt------qtplg 230
gi 23006958  300 WFVHSALNGrvHALREHVRNLAan--------rdNLRVRIFYTApeaqdrv-geaydipGPVTAewlva------ntpse 364
gi 149909197 150 WFIHVCDNSdvHAFKSEVNNIAves-------lgRIRTHLIYRHpthq-------deelGLFDSqdvidrqliqsllpld 215
gi 162146318 298 HYIHATRTPdtEAFGRHVRRLAae---------gRIRGDVFYSRrapadgedagtahhtGRMTMdwlra-------nlaa 361
gi 90023074  471 YFIHSTQNSetHTFANELKHLNkr--------hkWLQLYTIYSQpklndkl-gqhydvsGRISLdllrk------ilptd 535
gi 161383291 293 TYLHAAVDGdnLAMEGLSKSLAkrsvifletpsdADRAAARYDVe--------------GRITPdwlaa------ntnta 352
gi 58039327  298 HVVQIAHSAetAPFAERLAELShns-------agRIRVVTRLTEk-------------dGHPTAawvae-------qlpg 350
gi 150017144 152 HFIYSIPNSthHSFKEETVKLHn----------nNFKSNVFYTRpkenekl-gkdfdvqGRISEkwmid-------nlpk 213
gi 92112585  288 AYLHAARDGavHAFADELARLEady-------paLLEVITVYSQpraed-----apthgGHVDAnllsr-------ylpr 348
                        250       260       270
                 ....*....|....*....|....*....|....*....
Feature 1                                          #    
1GVH_A       356 TMQFYLCGPVGFMQFTAKQLVDLGvkqenIHYECFGPHK 394
gi 158424375 231 DADYFLCGPADFLRDMARGLHEAGvdrarIHYEFFGPPE 269
gi 23006958  365 QATYYLCGPKSFLASLANGLVRAGipaerVRFEFFGPAD 403
gi 149909197 216 DYDLYLCGTESFMSAMFQLFRSIGlpterIFYEFFGKAK 254
gi 162146318 362 DATYYVCGPDGFMRDAVSTLKAAGvpagrVRHEFFGSAH 400
gi 90023074  536 NFDVYLCGSESFMRSSYTNLKAMGiaesrIFYEFFDDGS 574
gi 161383291 353 LSDYYICGPKGFMAMAIAGLRAANvdmdrIHFEFFGPAE 391
gi 58039327  351 GVSTYLCGPTGFMHDMVHGLPAAGvpaerLRYETFGPDT 389
gi 150017144 214 DGDFYFCGPVPFMKTVYHNLISMAiekenINFEMFEAGV 252
gi 92112585  349 DAQCYLVGPAGFMRNMEAALSALGvaasrRHHEFFGPAE 387

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