Conserved Protein Domain Family
PDR_like

?
cd06185: PDR_like 
Click on image for an interactive view with Cn3D
Phthalate dioxygenase reductase (PDR) is an FMN-dependent reductase that mediates electron transfer from NADH to FMN to an iron sulfur cluster. PDR has an an N-terminal ferrredoxin reductase (FNR)-like NAD(H) binding domain and a C-terminal iron-sulfur [2Fe-2S] cluster domain. Although structurally homologous to FNR, PDR binds FMN rather than FAD in it's FNR-like domain. Electron transfer between pyrimidines and iron-sulfur clusters (Rieske center [2Fe-2S]) or heme groups is mediated by flavins in respiration, photosynthesis, and oxygenase systems. Type I dioxygenase systems, including the hydroxylate phthalate system, have 2 components, a monomeric reductase consisting of a flavin and a 2Fe-2S center and a multimeric oxygenase. In contrast to other Rieske dioxygenases the ferredoxin like domain is C-, not N-terminal.
Statistics
?
PSSM-Id: 99782
View PSSM: cd06185
Aligned: 197 rows
Threshold Bit Score: 151.096
Threshold Setting Gi: 88184847
Created: 24-Mar-2005
Updated: 17-Jan-2013
Structure
?
Program:
Drawing:
Aligned Rows:
 
Conserved site includes 11 residues -Click on image for an interactive view with Cn3D
Feature 1:FMN-binding pocket [chemical binding site]
Evidence:
  • Structure:2PIA: Burkholderia cepacia Phthalate dioxygenase reductase binds FMN, contacts determined at 3.5A
    View structure with Cn3D
  • Citation:PMID 1280857
  • Comment:PDR flavin/NAD binding domain homologous to ferredoxin reductase, but FMN binds rather than FAD.
  • Citation:PMID 7589982

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                            ## #                    ### 
2PIA_A        14 ASKEKIARd---IWSFELTDPQGap--------lpPFEAGANLTVAVPnGSRRTYSLCNDSqe-------rnRYVIAVKR 75
gi 28950015  258 VERRQETSr---IVSFILEAIDPivpppg-deksiLLKPGAHARVKLGnGLVRAYSIVGPSgysssstmtinHFQLGIAL 333
gi 145230708 444 VSHTAETPr---IVRLELEAVRRsap------spsAILPGSHARLKLPsGLRRAYSIVHGDt---------dRFTLGVAL 505
gi 111058363 261 VEKRQETSr---VTAFVFEAVDDian-------psPVEPGSHVRLKLGgKLVRAYSVVAGDs---------sRFELGIQL 321
gi 83770862  258 LTKVKETPr---IVSLVLRAITPsev-------psPIVTGSHVRVRLNeGLIRPYSVVTGDs---------nQFCLAVAL 318
gi 94313039   11 VVVSAIDEplpgVKRFVLSDQDHwp--------lpPFTPGAHIDLHLGgGLVRTYSLCNEPsd-------rnRYVIAVKH 75
gi 56708961   13 KETRDIAPg---VRLLRLSDPDGwd--------lpPFSPGAHIHIHLPdGLVRPYSLCGDAad-------rsSYFIAVQK 74
gi 88184847  276 VERTRETGr---VEGFVLEAVRKlgegkegegeevMLKPGAHVKVRLGnGLVRAYSVVGGDr---------nRFELGVAL 343
gi 145610963 253 VQKRRETAr---ITSLELEALQTad--------epELKPGAHVKLKLGnGLVRAYSIVGGDr---------nRFELGVAL 312
gi 171691781 241 VERKEQTKr---ITSFVLEAVDKfaeg-----eekELSPGAHAKIKLG-KGLVRAYSIVDGdk--------eRFQLAVAL 303
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            # ##                                                    #                   
2PIA_A        76 DsngRGGSISFIDDTseGDAVEVSLP-RNEFPLDKr-----------aKSFILVAGGIGITPMLSMARQLRae------- 136
gi 28950015  334 EphsRGGSAYFHSHVfeGHTLQVSTTfTNALSGNSag---------giSHHIYVAGGVGLTAFLTSLEYLQs-------- 396
gi 145230708 506 DersRGGSSYIHQTLkqGDTILVASEiRQGLTIDNm-----------aSHHIFIAGGIGITALIAMMKRLKd-------- 566
gi 111058363 322 SpesRGGSKYIHEQAkvGDVLIVGRF-TASFPLATd-----------aDEHVIISAGIGITAFLPAFKYLQq-------- 381
gi 83770862  319 DeasRGGSRYIHREVqpGDMLQCGPI-TASFPLSTv-----------aDHHVFIAGGIGITAFIAAAQHCEr-------- 378
gi 94313039   76 EvagRGGSAFVHERLdvGASIGVSLP-RGGIRTTDt------------GMNVFIAGGIGVTPFISTIRDLElr------- 135
gi 56708961   75 EpdgRGGSRQVHALQq-GERILVSLP-KNLLPLTNa------------PHVILIAGGIGITPFMPMIHEMSr-------- 132
gi 88184847  344 DeksRGGSRYLHETAvvGHEVEVGGF-TGAVPLREcgepscssttrprPSDLDIRRIIEVIPWNSQLYFCGpkrlmdea- 421
gi 145610963 313 AengRGASKYIHENVneGDVLTVGRF-TNGVPMVSa-----------aSSHVFVAAGVGITAFLPMIETMAa-------- 372
gi 171691781 304 EeksRGGSKYLHHEVkvGDVLQVGAI-TAAVPTVSa-----------aSHHVFVAGGVGVTAFLAMVCMSWkcsfrsqpt 371
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
2PIA_A       137 --------------gLRSFRLYYLTRDPe--gTAFFDELTSdewrSDVKIHHDHgdp---tkaFDFWSVFEKSKp--aQH 195
gi 28950015  397 --------------iHVSCELHYAVRSDe--dIPFRDRLSQln-pGTVKIYDKAkg-----erLNIEEIVKNIPw--nTM 452
gi 145230708 567 --------------tNQDYSLHYAVRSTt--dIAFQPLLIAl--qSHVTIYDKSqg-----qrLDIAKILRNRIw--nSH 621
gi 111058363 382 --------------tNQKFHLHYAHTGE----VPYASQIDAl--gSNATIYDKTqs-----qrLDVDKIISRTQd--rSH 434
gi 83770862  379 --------------lGYPYHLHYLVRSAe--dIALKEYLSGl--gSNVTIYDKSsg-----kvFNAKHTMEQIHe--gTH 433
gi 94313039  136 --------------gQTNYVLHWSSAGV----PSLVDMLGDaiaaGRVRLYDTRve-----saPDMDAILNACGe--nAK 190
gi 56708961  133 --------------qGRTFELHYASRQSd---AAFLPELEAlc-aGRMTRYDAErg-----qrLRLDDVISAIDa--tGH 187
gi 88184847  422 -------aketrargIAEGEMHFEIFEAdvsgDPFEAVVTNk---GGKPIRVGEeet----llECLQKEFGDIDs--sCC 485
gi 145610963 373 --------------iNYSVTLHYAVRSAe--dVPFRERVEKl--gDAVVLYDKTkgqrlkignIALRETQAAGIdekeVH 434
gi 171691781 372 rltkifykvefyksiHYSVVLHYAVRSAd--dVPFRDRIEElkkeGQLVLYDKAag-----qrMDIRQIIQSRGw--nSQ 442
                        250       260       270       280
                 ....*....|....*....|....*....|....*....|.
Feature 1                                               # 
2PIA_A       196 VYCCGPQ------ALMDTVRDMtg----hWPSGTVHFESFG 226
gi 28950015  453 VYFCGPKs-----LMQEAARQVqk---ygVPEGEVHFEAFE 485
gi 145230708 622 VYVCGPQ------RMIDDVIQTaag--vdMSSDEVHYELFN 654
gi 111058363 435 IYTCGPE------RLMDDVGNTakk--ygVPEACVHLEQFT 467
gi 83770862  434 VYCCGSE------RLQDSVLTVass--lgVSSSRLHFETFK 466
gi 94313039  191 AYCCGPM------RMLDAFERIve----gWPDARKHIERFA 221
gi 56708961  188 LYCCGPE------RMIGELLAKt-----gHMRDRVHVEYFG 217
gi 88184847  486 VGNCGTCrvslkgGRVDHRGTAlte--eeKATSMLACVSRG 524
gi 145610963 435 FEAFEADas--gdPFEATVVNKkggvtlkVGPDETLLEVMQ 473
gi 171691781 443 LYFCGPK------RLMDQAEREvke--lgVYQKEVHYEAFE 475

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap