Conserved Protein Domain Family
NOX_Duox_like_FAD_NADP

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cd06186: NOX_Duox_like_FAD_NADP 
NADPH oxidase (NOX) catalyzes the generation of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. ROS were originally identified as bactericidal agents in phagocytes, but are now also implicated in cell signaling and metabolism. NOX has a 6-alpha helix heme-binding transmembrane domain fused to a flavoprotein with the nucleotide binding domain located in the cytoplasm. Duox enzymes link a peroxidase domain to the NOX domain via a single transmembrane and EF-hand Ca2+ binding sites. The flavoprotein module has a ferredoxin like FAD/NADPH binding domain. In classical phagocytic NOX2, electron transfer occurs from NADPH to FAD to the heme of cytb to oxygen leading to superoxide formation.
Statistics
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PSSM-Id: 99783
View PSSM: cd06186
Aligned: 243 rows
Threshold Bit Score: 72.3374
Threshold Setting Gi: 50292097
Created: 7-Sep-2007
Updated: 17-Jan-2013
Structure
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Aligned Rows:
  next features
Feature 1:FAD binding pocket [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                               #                  ####  
gi 8134597   297 ITKVVMHP-----------SKVLELQMnkr-----------------gfSMEVGQYIFVNCpsi-----slLEWHPFTLT 343
gi 149248738 250 SLKIINVPs----------SSLQIVKIplpt--------------qnfpVWSPGSHIRINYsyani-kswiGPTHPFTLS 304
gi 146419762 222 FIKFQTYSatpvslkevpnSSLVLVSFpwpd--------------hiatSFKPGSHVRINHsnrsw-kswvFASHPFTSA 286
gi 50424351  240 ISDLKVISye--------gSNLIVVRIqrdl---------------kiaDWPSGSHIRLTYpltny-rswiFPTHPYTIA 295
gi 150863738 255 LHDISVVE-----------SPYSKMQIiripr------------petfpSYLPGSHIRLGYsatni-sawlYATHPFTIA 310
gi 50292097  256 TSRASLIEkied----rknTNLVVLKFprka---------------lpdFFNPSSHIRLSAyrklnplywiLPSHPYTLA 316
gi 1723936   248 IVFAKSLMilnkn-anyskTNLVHVRLprai---------------lpdYFEPGSHIRISPyrrinplywlLPSHPYTIA 311
gi 50312151  249 NVTQRVKDd----------TSLTYVKLdrs----------------airDWLPGSHLRLSKykkknplywlMPTQPYTIA 302
gi 71004640  325 ASRLRLAPsass----slvSSYMNLEDatkqcstsssrslcngpmlagaRIGAGDDVRITIprl-----qwVGDHPFSVF 395
gi 68469960  247 SLKVIDIPt----------STLQIIKIpqpn---------------kfpNWLPGSHIRLNYtiskf-kswtTATHPFTIV 300
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                         ###        ####                                                
gi 8134597   344 Sapee-------dffSIHIRAA------GDWTENLIrafe---------------------qqysPIPRIEVD------- 382
gi 149248738 305 Stye--------dqnKFLLLIM------RKTANFQFk----------------------------PEKTYLLT------- 335
gi 146419762 287 Tlpg--------tseTLDLVVKk-----GTFIFAKD-----------------------------TQYNLSSPyt----- 319
gi 50424351  296 Sles---------deMLDLIIS------KSNRFILH-----------------------------PQMPYSWT------- 324
gi 150863738 311 St------------nDDSESTLd-----LVMNKPVNfp-------------------------idITSPYTMT------- 341
gi 50292097  317 Tlpsd-------eivELIVNEHtv--pqFNTSSFVVe----------------------------LGYSYTIQ------- 352
gi 1723936   312 Slaed-------nsiDLIIKETstaepgSQIESLRS-----------------------------NPKSFHLDqektytl 355
gi 50312151  303 Slpde-------sqiDLIIREN------LTPYVVVGe---------------------------yTVVDVYST------- 335
gi 71004640  396 AvgrcktgnpdmgyvDLVIQRQa-----GLTQKLSQlaqelstyttdesrrpsdmylqqavrrkgQRVRVVID------- 463
gi 68469960  301 Tiped-------sanNLTLIVK------KPTQFVVY-----------------------------PLDSYLLT------- 331
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                 #                                                      
gi 8134597   383 -GPFGTas--edVFQYEVAVLVGAGIGVTPFASILKSIwykfqcadhnlktkkIYFYWICRETga-----------fsWF 448
gi 149248738 336 -GPYPAlpa-pfFSTLQVVNILCGGSGISFGLPLFQYFksts-------pntvINLVWSVRNKad------------lFI 394
gi 146419762 320 sLSFDDn----lISRFDQSVIICGGSGISLAIPVFKYLitk----------ldVTMIWCTRSK---------------AD 370
gi 50424351  325 -GPFTSfsk-elLQTIDNVDIICGGSGISFALPVFRNLkqk---------ashVKLIWCVRSN---------------ND 378
gi 150863738 342 -GPFPSlpp-qfYTTARHVNIICGGSGISFGLPIYKYFvnsn-------rsipIRLIWCIRSSdd------------tFI 400
gi 50292097  353 -NPHNPsvpdvcLQNANRIAIVCGGSGIAFGLPLFRFFksmk-------nisyLRLVWLTKNSsqlsi-----itksdSF 419
gi 1723936   356 iNSYPPsvpeecYSQGTNIAIICGGSGISFALPLFRHFfnke-------nvkyLKMIWLIKDYseyelvldylktnglTF 428
gi 50312151  336 -VPPSL------LYDSQRVAIVVGGSGISFGLSIFKYLqtrn--------ldyLKFIWLIRER---------------ED 385
gi 71004640  464 -GPFGRsp---sLEGAQHAILVAGGIAITFCYPLLVRAargef-----nsletCKLVWIVRNEgi-----------ldVL 523
gi 68469960  332 -GPYPSlap-pfFTTANIVNIICGGSGISLGLPIYHHFrsin-------stvpVKLVWTVRNQnd------------tFI 390
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 8134597   449 NNLLts--leQEMEelgkv-gflnyRLFLTGwdsnivghaalnfdkatdivtglkqk----------------------- 502
gi 149248738 395 RNSV------DLTDv----------QVYVTStidegetdenvnstqfvvedehdeenygllnndnndnnnneeevveeeq 458
gi 146419762 371 LFVLr----hYSLLdk--------vQVYITGndslsvedeseghglmhetielenleesskn------------------ 420
gi 50424351  379 LHVLr----tLNFNee--------iIIHITGnlqdstsntifdeedyglldydnnenfeleslpssetpsrtv------- 439
gi 150863738 401 LDHLlp---eRSIDv----------QIYITSntgslnqqqsastifdeeadallegdsttniemanlat----------- 456
gi 50292097  420 RALI------SEDGkid------nlDIYLTNkttaptssgteetendieledfelnttedlte----------------- 470
gi 1723936   429 EKKL------SNNKr---------iSVFISGeytaetrldeittniddenseyemgsfnnededlsisnfnsenadsnd- 492
gi 50312151  386 MHILnhcnfeTNDL-----------EVYVTRslppddtqtksmnangssefddidfeletmdesgal------------- 441
gi 71004640  524 RDSLse--llDEIRnrggsrcslsiDIYTTVkasapslggsiavdmks-------------------------------- 569
gi 68469960  391 MNQL------DMTGv----------QVYVTSigdtnsesqenqqhqavplfvieeeeeeeghgllnnesengielqnmpe 454
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 8134597   503 -------------------------------------tsfgrpmwdnefstiatshpksvvgVFLCGPrtlaKSLRKcch 545
gi 149248738 459 qqegielhtlhkqssgtdasttskegtkllkvgenywlgrpkldevfalhnptlthdpkcswVVACGP----DGLIKdak 534
gi 146419762 421 -------------------------------seatkqpeilrgrpdlnnvcaslettpnsscVISCGP----RSLVKdce 465
gi 50424351  440 --------------------nddslsqdtrpkkeskfiivqgrpdfgntfeslvqvnssnkwIIACGP----RSLVNsaq 495
gi 150863738 457 -------------------------dkeekktdhfntihdgrpnfddafgnlaaavdvdekwLIACGP----RKLIDdcr 507
gi 50292097  471 -------------------------------ykklcnitegermdwttelsslveedtsktwLLACGP----EGLVTdgk 515
gi 1723936   493 ---------------ntpetshsptkengsmievkskhsftlsnelksfnnesaqvnqnetwLFSCGP----PSLLQlsk 553
gi 50312151  442 --------------------------ltndskspslvhfgrrldwqvdlaqfieshalqnswLVCCGPe---SLLTDger 492
gi 71004640  570 ----------------------------------------------aaqlpsrpeatqlrskLMSAGStssiAASSKe-- 601
gi 68469960  455 sn----------eensetntinsknnkdneerkeyfkfgrpkfdevfaiddpttthdldnswVIACGP----DELISdak 520
                        410
                 ....*....|....*....
Feature 1                           
gi 8134597   546 ryssldprkvqfyFNKENF 564
gi 149248738 535 twa----kdhnyqFFSEKY 549
gi 146419762 466 nwc----rnhkieSVTEIY 480
gi 50424351  496 ewa----senkvnFVSEIY 510
gi 150863738 508 kwt----kgkdieFYSELY 522
gi 50292097  516 sya----nangihFASEVY 530
gi 1723936   554 kyc----nderinFVCETY 568
gi 50312151  493 yak-----qnycnFVKEFY 506
gi 71004640  602 -------------FGSSHQ 607
gi 68469960  521 rws----kdrgyrFYYEKY 535

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