Conserved Protein Domain Family
O2ase_reductase_like

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cd06187: O2ase_reductase_like 
Click on image for an interactive view with Cn3D
The oxygenase reductase FAD/NADH binding domain acts as part of the multi-component bacterial oxygenases which oxidize hydrocarbons using oxygen as the oxidant. Electron transfer is from NADH via FAD (in the oxygenase reductase) and an [2FE-2S] ferredoxin center (fused to the FAD/NADH domain and/or discrete) to the oxygenase. Dioxygenases add both atoms of oxygen to the substrate, while mono-oxygenases (aka mixed oxygenases) add one atom to the substrate and one atom to water. In dioxygenases, Class I enzymes are 2 component, containing a reductase with Rieske type [2Fe-2S] redox centers and an oxygenase. Class II are 3 component, having discrete flavin and ferredoxin proteins and an oxygenase. Class III have 2 [2Fe-2S] centers, one fused to the flavin domain and the other separate.
Statistics
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PSSM-Id: 99784
View PSSM: cd06187
Aligned: 32 rows
Threshold Bit Score: 205.905
Threshold Setting Gi: 158430640
Created: 21-Feb-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:FAD binding pocket [chemical binding site]
Evidence:
  • Structure:1KRH: Acinetobacter sp. Benzoate dioxygenase reductase binds FAD, contacts determined at 4A
    View structure with Cn3D
  • Structure:1TVC: Methylococcus capsulatus Methane monooxygenase binds dihydroflavin-adenine dinucleotide, contacts determined at 4A
    View structure with Cn3D
  • Comment:contacts lie primarily in the N-terminal region

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                       #           ####               ### #   ###       
1KRH_A       113 LARVENLSdsTITFDIQLddgqpdihFLAGQYVNVTLPGttetRSYSFSSQPgn---rLTGFVVRNVpQGKMSEYLSvqa 189
gi 119898141 109 VSRIESLTptIKGVWVKLdepe-gmrFQAGQYINLHLPDgigtRAFSIASAPsa--agEIELNIRIVaGGQGTAYVHekl 185
gi 1840137   119 IVATEPLThdILKVTIQTdrp---vhYLAGRYANVRVPGwprfRCYSFANAPqrkgrnVLEFYIRKVpAGEFTEALFrge 195
gi 2828014   103 VTAIDEAThdIRRLRIKLakp---leFSPGQYATVQFTPec-vRPYSMAGLPsd---aEMEFQIRAVpGGHVSNYVFnel 175
gi 3914147   103 VVAVESPThdIRRLRVRLskp---feFSPGQYATLQFSPeh-aRPYSMAGLPdd---qEMEFHIRKVpGGRVTEYVFehv 175
gi 152996681 102 IVAYDVLSndVRSLKLKInkg---feYSPGQFANLTFWRedgtRSYSMAGVTed---dLLEFHIRLVpNGRVTSKLDehi 175
gi 54024197  110 VVSLDPIArdTRRLVLEVdga---ldFLPGQYAQLHVPGtecwRPYSMANSPar--phRLEFHIRYSdGGVATGWIFhgl 184
gi 153011369 108 VIGLYDLThdIKLIRIAPadgs-slpFTAGQYAQIRFTGtp-vRSYSMANRVge---gSLEFHVRRVaGGITSEHIHgal 182
gi 159184661 109 VEEAVDAThdIKLIRIRLedra-qftFKPGQYVRLLYPDss-pRDYSIASRVde---eLIEFHIRHVpGGMTSGRIFsla 183
gi 33469579  109 VVTLEALThdIRRVRIRLadeq-plmFFAGQYADIKFGQap-aRSYCMANRPge---aELEFHIRRVpGGVASAYVHtvl 183
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                       #  #                                             
1KRH_A       190 KAGDKMSFTGPFGSFYLRdv-kRPVLMLAGGTGIAPFLSMLQVLEQkgseHPVRLVFGVTQdcDLVALEQLDALqqk-lp 267
gi 119898141 186 KAGDAIRITGPYGRFFVKksakVPVVFMAGGSGLSSPRSMILDLLEegfdLPITLVYGQRSraELYYHDEFLALaqr-hp 264
gi 1840137   196 LDGQPLEMEAPQGTFHLHgg-dAPMLCIAGGSGLAPLVSILEHARAnrikRDCILLFGARTegDLYQLEAIGNIaanwqg 274
gi 2828014   176 SVGASVRISGPLGTAYLRrthtGPMLCVGGGTGLAPVLSIVRGALEsgmsNPIHLYFGVRSeqDIYDEERLHALaar-fp 254
gi 3914147   176 REGTSIKLSGPLGTAYLRqkhtGPMLCVGGGTGLAPVLSIVRGALKsgmtNPILLYFGVRSqqDLYDAERLHKLaad-hp 254
gi 152996681 176 EIGASIKLNGPLGASYLRrknnDPMLCVATGTGLAPILSIVRGALEsgmkNDIHLVFGARTeeDLYGLGYLDHLate-yt 254
gi 54024197  185 TVGDRVELRGPLGDFVLDda-dHPLLLLAGGTGLAPLSAMLRAALErghrSPIHLYHGVREeaDLYDVEWLSELsrs-hp 262
gi 153011369 183 KSGDKVELEFPLGSSYLRqnhsGPMLCIAGGSGLAPIKSIVETALAhgmkQPIHVYFGARSecDLYLVEHFQSLaeq-ya 261
gi 159184661 184 RAGDPVTIVGPFGSSFLRekhcGPILGIAGGSGLAPVKAVVEAALAtgreRPVHVYFGARAerDLYMLDRFQDLttr-hg 262
gi 33469579  184 EPGERVTLELPKGTSYLReghgGPMLCIAGGAGLAPIKSIVETALAsgmsQAIHVYFGVREgrDLYGLEEFRALard-yp 262
                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
Feature 1                                                                             # #
1KRH_A       268 WFEYRTVVAHaes---qheRKGYVTGHIeydwl-nggevDVYLCGPVPMVEAVRSWLDTQGiqpaNFLFEKF 335
gi 119898141 265 NFRYVPALSHepegsdwngFRGFVHEAAkaafdndfrghKAYLCGPPLMIDACITTLMQGRlferDIYMEKF 336
gi 1840137   275 EFRFIPVLSHepehsdwtgARGLVTEHIpadf---cegaEGYLCGPPPMIDAAIARLLDNRlpleKIFYDKF 343
gi 2828014   255 NLKVNVVVATgpag--pgrRSGLVTDLIgrdlp-nlagwRAYLCGAPAMVEALNLLVARLGivpgHIHADAF 323
gi 3914147   255 QLTVHTVIATgpin--egqRAGLITDVIekdil-slagwRAYLCGAPAMVEALCTVTKHLGispeHIYADAF 323
gi 152996681 255 NFQYLIALDHanpn--stsFRGLVTDAIaahfp-elknwRIYLAGAPAMVEAASLACTKRGadieRIYADAF 323
gi 54024197  263 DFHYTPCLSRgew----ngRTGYVGDAVladfd-nlrdySGYLCGPPAMVEAAGRALKRRRmaprRIFREKF 329
gi 153011369 262 NLTFVVVLSEaea---tqyRCGFVTKAVaddlm-dldgwKAYVAGPPGMVDAAIQLALARGlrgeDMHADVF 329
gi 159184661 263 NLSFVPVLSNedh---aniRRGYVGAAVaddfd-dldgwKAYIAGPPAMIEATVPQLLARGmrtaDIHADVF 330
gi 33469579  263 TLLFTPVVSGipv---ahcSRGLVTDIAgseqa-dlrgwKVYVAGSPTLVDATIGMALKRGlkiqDLHAQIY 330

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