Conserved Protein Domain Family
DHOD_e_trans_like

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cd06192: DHOD_e_trans_like 
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FAD/NAD binding domain (electron transfer subunit) of dihydroorotate dehydrogenase-like proteins. Dihydroorotate dehydrogenases (DHODs) catalyze the only redox reaction in pyrimidine de novo biosynthesis. They catalyze the oxidation of (S)-dihydroorotate to orotate coupled with the reduction of NAD+. In L. lactis, DHOD B (encoded by pyrDa) is co-expressed with pyrK and both gene products are required for full activity, as well as NAD binding. NAD(P) binding domain of ferredoxin reductase-like proteins catalyze electron transfer between an NAD(P)-binding domain of the alpha/beta class and a discrete (usually N-terminal) domain which vary in orientation with respect to the NAD(P) binding domain. The N-terminal domain may contain a flavin prosthetic group (as in flavoenzymes) or use flavin as a substrate. Ferredoxin is reduced in the final stage of photosystem I. The flavoprotein Ferredoxin-NADP+ reductase transfers electrons from reduced ferredoxin to FAD (forming FADH2 via a semiquinone intermediate) which then transfers a hydride ion to convert NADP+ to NADPH.
Statistics
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PSSM-Id: 99789
View PSSM: cd06192
Aligned: 30 rows
Threshold Bit Score: 157.874
Threshold Setting Gi: 150020171
Created: 22-Feb-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:FAD binding pocket [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                # ####             ###   
1EP2_B          9 VVSQREVay-----nIFEMVLKgtlvdemDLPGQFLHLavpn---------gamLLRRPISISSwdkrakTCTILYRIGD 74
gi 118047057    9 IRERRQFd------hLVWKSLIapeiartARPGQFVLVrvsp--------sfdpLLWSVFFLAGadphsgTVELLIDPRD 74
gi 159901242    5 ILDQQQLnq-----hYGLLTVAspq-lasAQPGQWAMLhhgls-------sdpfLRSRAWIIATnr--ssTATLALDLQD 69
gi 56416687   822 VTSVTYPar-----nIVELTIHspiaarkFKPGQFYRLqnfgsahpyakhtqllMEGVAVTGASvdkengTITVVVLDVG 896
gi 29653924   852 VVDVRRHtp-----dMVECRIRapmaarnFRAGQFYRLqnyeclspliadtrlqTEALAMIGVNesaeadVISFIVLERG 926
gi 88608202   937 LLKAEVLevialtnnIYELRICaplaarnFMPGQFYKLqnfh--------spfqIEAIALTGSCvdknngVISLIVIEVG 1008
gi 53756097   914 IVAVNRLtp-----tIIEVVVRapaaakhFQPGQFYRVqnyealapvvegtvlaTEGIALTGAWvdkdngLISLIALEMG 988
gi 30248862   864 VHKVERLts-----nIIEVVVHaplaarhFHPGQFYRFqnyaslagiseqtrlaMEGIALTGASvdvsngLVSLIALEMG 938
gi 83591905   850 VHAITRHtp-----tIVEITLHapqaarrFRPGQFYRLqnferqaarvdgttlaMEGLALTGAWvdreagLLSVIVLEMG 924
gi 58617045   747 ILDVEHLtk-----nITKLTILaplaaanFKPGQFYRLqnfeynslnientklsIESLALTGVSvdkdkgTISTIVLNAG 821
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1           ###                                         #                                 
1EP2_B         75 ETTGTYKLsklesGAKVDVMGPLG--NGFPVaevtstDKILIIGGGIGVp---PLYELAKQlektgcqMTILLGFASen- 148
gi 118047057   75 SAGGWLAGyp--vGTQLDVCMPVVp-AVTPNlt---tQTLLLAGVGPTLp---ALLFTARQyaa--rlSISLWLTGDe-- 141
gi 159901242   70 PLLASIGRlp--kGFELDCIGPLG--RPLSSdg---qKATLLIAEGNAIy---SLLLYAQRvtetgspVLLLASANDa-- 137
gi 56416687   897 GSSKLCSQlr--dGQLVSLMGPVG--TPTEIps---dENVMLVGGGVGNa---VLFAVGKAliangckVLYFAGYRSles 966
gi 29653924   927 GSSRLVATlk--pGQPISIMGPTGcyAKIPEgn---sKTIMIVGSSMAAahlrSLGPALRKag---hrVFYVALIEKae- 997
gi 88608202  1009 VSSYLCKFlk--vGERVVLTGPLG--TPTEIpv---nENVLLIGGGVGNa---VLFSVAEAmkkngcnVLYFSGYKKln- 1077
gi 53756097   989 SSSRLCAQwk--pGDPLVVMGVTG--APTEIps---gQTVALVGGGLGNa---VLFSIGKAlrsagnqVIYFAGYRNsa- 1057
gi 30248862   939 GSANLCALlq--pGEPVVLMGPTG--TPTEIes---hETVVLVGGGLGNa---VLFSIGAAaraagckVLYFAGYKKla- 1007
gi 83591905   925 GSSDLCAHlr--lGEPVVLMGPTG--SPTEIga---gETVLLAGGGLGNa---VLFSIGKAmraegckVLYFAGYKGla- 993
gi 58617045   822 GSSHLCNYlk--kNEPIIFMGPTG--TPTEIps---nKNVMLIGGGVGNa---VLFSIGQAllshnckVLYFAGYKKt-- 889
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
1EP2_B        149 -vkILENEFsnl-knvTLKIATDDGsygtkghvgml-----------------------mneidfevdaLYTCGA----P 199
gi 118047057  142 --gYLPPPFllp--atVEVQTSTGGaaalfnlltvs----------------------rvdnpirwadqIIFALP----P 191
gi 159901242  138 ---MRVPPFvlp--aeIEYLATADDvldllessak------------------------ldaplvwanrLLAAGSldlaS 188
gi 56416687   967 vfgIRSIEAas----hTTVWCCEDGaikherpedishhgniida------iqaysaaknstiklqsvdrIIMVGS----S 1032
gi 29653924   998 -elYCQEELeaa--sdSILWVSEKDehvdlhrpqdkamqgelis------alrnytlekpaiplesvdqVYVIGP----A 1064
gi 88608202  1078 -drFKADMIeaf--sdCVVWVCDEGkfpsnrtcdvslmgnmimal-----ekyirgeillpikagaldrIVTIGS----D 1145
gi 53756097  1058 -dvFKAEELeaa--sdLLVWTVDKRegntpipitrpddksfmgniveamlayakgelgptpihlddvdhMIVIGS----D 1130
gi 30248862  1008 -drYKVAEIeaa--adTIVWCSDEApgfqpsrlgdlsyvgnivqam---vayasgelgsapismqaadrIIAIGS----D 1077
gi 83591905   994 -drYRIDDIhaa--adTIVWCCDETpgfvperpgdftvtgtivdam---vayaegrlgtppialsevdrVIAIGS----D 1063
gi 58617045   890 --eDIFEPSsieksssNVIWCCNEKrieprrtqdqsyhgniieai----eqyqnhtsqgtniplhsidrIIMIGS----S 959
                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
Feature 1                                      ##                                      
1EP2_B        200 AMLKAVAKKydq--------lerLYISMESRMACGI-GACYACVEHdked----eshALKVCEDGPVFL 255
gi 118047057  192 QLFGMAVEAirtvrl--rwehgfAQVIVAGPLPCGL-GICRVCQIEtr-------hgWQRCCIEGPVFD 250
gi 159901242  189 RLSQRIRREryaw------qqgfAAFIVDQAYPCGI-GMCGGCWQStr-------qqPKLLCHHGPALD 243
gi 56416687  1033 GMMSAISSAvrgklrscfkeqikIVASINSPMQCMMgGICGQCIQRhinps-tgeesFVYSCVNQDQCA 1100
gi 29653924  1065 SLLRGIQKAregllkeyfkpkteFIASVYGPMQCMMkGVCAQCLQWqidpatgkrtkAVYACSWQHQPM 1133
gi 88608202  1146 SLMSAVQRFidkhrhi-lkpnlkAIASINSPMQCMMqGICAQCIQRrhvn---gkeeYVYSCVNQDQNM 1210
gi 53756097  1131 RMMEAVKHArhgllapylkphhaAIGSINSPMQCMMkGVCAQCLCKhvdpe-tgreyFVYSCYNQDQEL 1198
gi 30248862  1078 RMMAAVAAArhqqlapylkvehfAVGSINSPMQCMMkEICAQCLQPqkdpv-tgdisYVFSCFNQDQPL 1145
gi 83591905  1064 RMMRAIAEArhgvlaphlnsrhvAIGSINSPMQCMMkEICGQCLQPltdpq-tgetrIVFTCFNQDLPL 1131
gi 58617045   960 HMMDAVSYAifnqyrhffkqdikVIASINSPMQCMMkEICAQCLQKhinpi-tkeeyFIYSCKNQDQPA 1027

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