2GPJ


Conserved Protein Domain Family
siderophore_interacting

?
cd06193: siderophore_interacting 
Click on image for an interactive view with Cn3D
Siderophore interacting proteins share the domain structure of the ferredoxin reductase like family. Siderophores are produced in various bacteria (and some plants) to extract iron from hosts. Binding constants are high, so iron can be pilfered from transferrin and lactoferrin for bacterial uptake, contributing to pathogen virulence. Ferredoxin reductase (FNR), an FAD and NAD(P) binding protein, was intially identified as a chloroplast reductase activity, catalyzing the electron transfer from reduced iron-sulfur protein ferredoxin to NADP+ as the final step in the electron transport mechanism of photosystem I. FNR transfers electrons from reduced ferredoxin to FAD (forming FADH2 via a semiquinone intermediate) and then transfers a hydride ion to convert NADP+ to NADPH. FNR has since been shown to utilize a variety of electron acceptors and donors and has a variety of physiological functions including nitrogen assimilation, dinitrogen fixation, steroid hydroxylation, fatty acid metabolism, oxygenase activity, and methane assimilation in a variety of organisms. FNR has an NAD(P)-binding sub-domain of the alpha/beta class and a discrete (usually N-terminal) flavin sub-domain which vary in orientation with respect to the NAD(P) binding domain. The N-terminal moeity may contain a flavin prosthetic group (as in flavoenzymes) or use flavin as a substrate. Because flavins such as FAD can exist in oxidized, semiquinone (one-electron reduced), or fully reduced hydroquinone forms, FNR can interact with one and two electron carriers. FNR has a strong preference for NADP(H) vs NAD(H).
Statistics
?
PSSM-Id: 99790
View PSSM: cd06193
Aligned: 143 rows
Threshold Bit Score: 84.6223
Threshold Setting Gi: 149276609
Created: 29-Apr-2008
Updated: 17-Jan-2013
Structure
?
Program:
Drawing:
Aligned Rows:
 
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:FAD binding pocket [chemical binding site]
Evidence:
  • Structure:2GPJ: Shewanella putrefaciens Siderophore-interacting protein binds FAD, contacts determined at 3.5A
    View structure with Cn3D
  • Comment:contacts lie primarily in the N-terminal region

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                 ####   
2GPJ_A        13 VIRSTYITPh--XLRITLGGagla--gfpaDQESAYIKLLFPqag-----------------------erPLXRTYTIRQ 65  Shewanella putr...
ZP_01882752   24 ITNVEFISQt--VKKIHLNGdfk----dlsFPVGCYIDFRVSd---------------------------TEARRYTVAK 70  Pedobacter sp. ...
ZP_01883177   25 VSSVTLLTDt--LCQLNLSGdfq----gldFHPGYAIDFRVNd---------------------------NDFRHYTPSA 71  Pedobacter sp. ...
YP_881575     24 VSEIEALTPr--MRRIRFSGprlh---glgWTPGQHVRLQVEslrqsv----------------lrlhpyPVLRTYSIYA 82  Mycobacterium a...
YP_678588      2 VISKEQIADn--VFHLIIAGkalq---nlaYIPGQQVRIFIGmdkk--------------------lglrEKIRTYSIWN 56  Cytophaga hutch...
YP_715755      7 VAAVEPLARr--TRRIVLAGeplt---glaWTPGQNVTVFLAdptslas---------------wrhgprDLKRSYSVWN 66  Frankia alni AC...
YP_001342377  20 VIKLKDLSSt--LRRVTLQGedfqgfgfhpLAPEAHVKLFFPnaaddvlrmpevlddgnadwqgaaegrfSAFRDYTIRS 97  Marinomonas sp....
ZP_01089606   16 VDSIEELNPk--FRLITLNGdalk---nvvWTPGDKIQIQLGg---------------------------WVQRTYTPLD 63  Blastopirellula...
YP_001196358  23 VNVTTIETPspnIKIINFKGnfp----hikFKLGQAVVIRIDd---------------------------TNYRNYTPSK 71  Flavobacterium ...
YP_661831     10 VTRVESLSPh--LRRIVFSSeela--gfpqGKEGAHVKVLVPhpgeqfpe------------lafdapkpAFKRSYTIRS 73  Pseudoalteromon...
Feature 1                  # # #       ####                                     #                
2GPJ_A        66 qr--dDEIDVDFVLHDt----dGPASSWAKTaQVGELIQIGGPGlkkli-nfeaDWFLLAGDXTALPAISVNLAKLpnn- 137 Shewanella putr...
ZP_01882752   71 adrenGFLEFIVHVHGa-----GSGSQFMQElKPGDKIKMNKPRternyydrsaNRFIVFGDETSLALACSFLPVLkqn- 144 Pedobacter sp. ...
ZP_01883177   72 fdaqeGTCEILIHLHGn-----GPGSDFISRlKVGEVLKMVIPRgrkly-qpdhQFHFFFGDETALGLFKLLREEIhaa- 144 Pedobacter sp. ...
YP_881575     83 adpdrGALDIVMVDHDgdpkgaTPARRWAMAaTLGDHVRMTRPQgkfvi-rddaPYHVFAGEETASVAFAAMLRSLppt- 160 Mycobacterium a...
YP_678588     57 yhsgnGTMNLTICAHSg-----GPGSNWIKSvKAGEEIYLTAPQgnfll-nvhaSEYLFIGDATTLAHFYAIRRNLpdtk 130 Cytophaga hutch...
YP_715755     67 yd-paGRLELAVYDHGg----eSPGASWSRTvQAGDGVIVTKPDgrlva-rpgaSLHLFAGDDTAAVPLGAILRSLpet- 139 Frankia alni AC...
YP_001342377  98 fdadlLTVDIDFVLHDv-----GVGGPWAKQaKVDDLLGIFGPRsvrlp-pldaLKYVFFVDETSLPALARWLEILpes- 170 Marinomonas sp....
ZP_01089606   64 wepdeGRTRILVYLHAe-----GPGTRWACSlRVGDACPLFGPRrsinl-tqlrRPAVFFGDETTFGLARALRATTset- 136 Blastopirellula...
YP_001196358  72 wdsenGTFEIIFHIHHn-----GPGSAFINNlKPSDQITVGMPRgfkif-nneaQYHFFFGDETCISVFKSMKDEInrt- 144 Flavobacterium ...
YP_661831     74 fdagsSELTIDFVVNRh----aGPATNWASAaKVGDFAAIAGPGapkls-nyneDNYLLIGDLTSINAVNGFARYIpkq- 147 Pseudoalteromon...
Feature 1                                                                                        
2GPJ_A       138 -avGYAVIEVLSe-----------aDIQPLVhpe----------------hvELHWVInpeadpegrplve-----riaq 184 Shewanella putr...
ZP_01882752  145 nqdFHFYFELDDqnk-------napELLGLEnyttfs---------kngsfrNEEWIAelplfqt--------------- 193 Pedobacter sp. ...
ZP_01883177  145 dqhYTGVLELSE-------------ENLALParl----------------gmRLKTVTknpddpgryaaasl--hamktg 193 Pedobacter sp. ...
YP_881575    161 -aeVYGVVEAATe-----------aDHLPLArpleqverggapaansavladALRRLPlpd------------------- 209 Mycobacterium a...
YP_678588    131 imrGIIYAEKKSqlftdl-dgllpfEFYELPhnp----------------ieQIKELIelkppv---------------- 177 Cytophaga hutch...
YP_715755    140 -arCFAVFECNEp-----------aDEIPLPga------------------sTPTWVHrhgasaassallp----aavag 185 Frankia alni AC...
YP_001342377 171 -anIEAWIEVHSa-----------sSEISLPahv----------------gaNLHWLHrnneqenhlygrllldaldnlp 222 Marinomonas sp....
ZP_01089606  137 -egVEFLFEVSTls----------eSVQTLEhlg---------------ianAQHCVRadddahlpelet------rmlg 184 Blastopirellula...
YP_001196358 145 ennYLGILELDEvsadipkklglivDIVPKSvnk---------------aewAIKSLHnldteiw--------------- 194 Flavobacterium ...
YP_661831    148 -anLKSIITVPTr-----------aDVIAMDage----------------hlNIEWHIedesshtleqhv------erva 193 Pseudoalteromon...
Feature 1                                                 ###
2GPJ_A       185 lpwlagepAVWIACEfnsXRALRRHFKQAHalpkshFYTSSYWK 228 Shewanella putrefaciens
ZP_01882752  194 --adfqkaNFVLTGNaksAQSFRKVAKGKGaq---kTYLHGYWL 232 Pedobacter sp. BAL39
ZP_01883177  194 yphifhqgIFYLIGNaasIQHVKRTLRHMGvav-knIRTEAYWM 236 Pedobacter sp. BAL39
YP_881575    210 -----hpgVAYLAGEartVQALRQILITERgwkrnqVRTKPFWS 248 Mycobacterium avium 104
YP_678588    178 ---ntadaIVYIGGDgriCVALNTYFRKFKswktknIRIKPFWM 218 Cytophaga hutchinsonii ATCC 33406
YP_715755    186 ldlpaepgVAYVAGEartVQAVRDHLVNDRgwprrdVLTQPFWT 229 Frankia alni ACN14a
YP_001342377 223 sgwacektWVWAATEahaIVQLRRKLMNIDalnkdhLDLVAYWK 266 Marinomonas sp. MWYL1
ZP_01089606  185 lldsqqpaQFVLAGRstaIQRIRQALRQRScss-aqFHTRAYWA 227 Blastopirellula marina DSM 3645
YP_001196358 195 --elwkkgCFYLMGNaksIQAFRKALREKGvsn-knIHTQPYWA 235 Flavobacterium johnsoniae UW101
YP_661831    194 kimpcdntQVFFGLEarsVRTIKGILLDDLgyermnLFATGYWK 237 Pseudoalteromonas atlantica T6c

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap