Conserved Protein Domain Family
FNR1

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cd06195: FNR1 
Click on image for an interactive view with Cn3D
Ferredoxin-NADP+ (oxido)reductase is an FAD-containing enzyme that catalyzes the reversible electron transfer between NADP(H) and electron carrier proteins such as ferredoxin and flavodoxin. Isoforms of these flavoproteins (i.e. having a non-covalently bound FAD as a prosthetic group) are present in chloroplasts, mitochondria, and bacteria in which they participate in a wide variety of redox metabolic pathways. The C-terminal domain contains most of the NADP(H) binding residues and the N-terminal domain interacts non-covalently with the isoalloxazine rings of the flavin molecule which lies largely in a large gap betweed the two domains. Ferredoxin-NADP+ reductase first accepts one electron from reduced ferredoxin to form a flavin semiquinone intermediate. The enzyme then accepts a second electron to form FADH2 which then transfers two electrons and a proton to NADP+ to form NADPH.
Statistics
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PSSM-Id: 99792
View PSSM: cd06195
Aligned: 53 rows
Threshold Bit Score: 210.114
Threshold Setting Gi: 41614847
Created: 23-Mar-2005
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 13 residues -Click on image for an interactive view with Cn3D
Feature 1:FAD binding pocket [chemical binding site]
Evidence:
  • Comment:contacts lie primarily in the N-terminal region
  • Structure:1A8P: Azotobacter vinelandii FNR binds FAD, contacts determined at 3.5A
    View structure with Cn3D
  • Citation:PMID 9865948
  • Structure:2BGI: Rhodobacter capsulatus FNR binds FAD, contacts determined at 3.5A
    View structure with Cn3D
  • Structure:1FDR: Escherichia coli FNR binds FAD, contacts determined at 3.5A
    View structure with Cn3D
  • Citation:PMID 9149148

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                        #                              ####             
1A8P_A         8 RVLSVHHWNDt--LFSFKTTrnp-slrFENGQFVMIGLevd-----------------grpLMRAYSIASPNye------ 61
gi 68550654   24 TVVGKIMVTPd--LMIFRIHtdeareeFEAGQNLLLGLygfekrssnse-pelvpaegeklIRRPYSLASEKtet----- 95
gi 168698110  17 TVVSLRLVNPd--LMILRVKpdaprpeHRPGQYCTLGLgywerrtegcqaeslsegdftkvVRRAYSLSCGIldddgdll 94
gi 32476445   23 TIIKRMDLTEd--LARFRIQcdepvmpFEPGQYVAIGLgnweprlrgtqpedvpikksrklVRRAYSISCPMlhdaespk 100
gi 149179292  26 SVMDLRMPHDh--LMIVRIKpdedvprFSGGQYTTLGLgswehrvdgg---plaelekpklIRRAYSISCPMldlqgdll 100
gi 41614847    4 KILKREFIDKd--HYLLWVErpk-dynYKPGQFTYIEStin-----------------nnkVRRFYSFASHPee------ 57
gi 160872630   9 TLEKSEQIAPdvkHFTFYYEegg-cfqFIPGQFITLHIptd-----------------ekiLRRSYSIANKTypa----- 65
gi 58424599   30 KLVDRRMIAPtvaHCQFVRDdgq-pldFQPGQFIQIHFdyad----------------gtpTKRSYSLATIHdhalg--- 89
gi 88812917    9 VLQAARMITPrvrELTFVREdrs-pldYTPGQFITLFIesg-----------------gerLRRSYSIASIPgse----- 65
gi 52840716   13 TLEESFMISPkvkHFIFNCElsp-pfeYLPGQFITIHFehd-----------------gknLKRSYSIANEPkkd----- 69
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                       # # #          # ##                                       #      
1A8P_A        62 ----------ehLEFFSIKVQn--------GPLTSRLQHLKeGDELMVSrKPTGTLVTSdllpgKHLYMLSTGTGLAPFM 123
gi 68550654   96 ----------rqLEFYISQVKs--------GQLTSRLFNLNvGERLYVGtKITGIFRLDetpdgSDIVMVATGTGIAPYI 157
gi 168698110  95 -----rledsdwLEFYIVLVRenpd--grvPALTPRLFALSeGDRIYLGdRVTGHYTLDpvrpcDTVLFLGTGTGEAPHN 167
gi 32476445  101 agelapvdqidyLEFYITLVRqgataaskpPALTPRLFGKGeGDRICVErKITGRYLLGdipadENVLMLGTGTGEAPHN 180
gi 149179292 101 -----andeidyLEFYITLVLrpd---tddPPLTPRLFRLKeGDRLHLGkKPVGTYTLKpvqpgDNVIFAGTGTGEAPHN 172
gi 41614847   58 ----------enLLFYIKRVPn--------GLMSNYLAEIPlGSTIEIG-NPMGHFTLDra-dtDRVIFIGAGTGIAPIR 117
gi 160872630  66 ---------tnkIEFAASYVPs--------GVASQLLFTLKpGDKVMAT-GPFGRLTLRee-mpERYILVATGTGVTPYR 126
gi 58424599   90 --------pgeaVEIAVSFVPg--------GSATALFEGLEiGDQLQAS-GPYGRFCLQpgdhnQRYVLIATGTGVTPYR 152
gi 88812917   66 ---------seeIRIAATYVEg--------GRATARLFKIEpGERIQAM-GPFGRLVLRed-ppGRYLLVATGTGVTPYR 126
gi 52840716   70 ----------nrIEFAAGYFEg--------GPGTELLYNLKpGDVIHIN-GPFGRLIFRde-tpGRYILVATSTGTTPYR 129
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1A8P_A       124 SLIQDpe---vyERFEKVVLIHGVRQvNELAYQQFITEHlpqseyfgeavkeKLIYYPTVTRESFh---------nQGRL 191
gi 68550654  158 SFLRShi---veRPESKMVVIQGAAHrWDLGYYSELTFLeks--------faNFFYVPTLTDADDrwd------gyRYCI 220
gi 168698110 168 YMTWEll---srRHTGKIVNVCCVRYaRDLGYHNTHQLLaqk--------fpNYSYIPLTTRELGnt--------rKLYI 228
gi 32476445  181 AMATTll---sgGHRGKVVIATSVRYqSDIAYQSEHDELmrr--------fpNYCYLPLTTREPKnlepnrpdyvgKQYL 249
gi 149179292 173 SMSIEll---krGHTGRIVSMTCVRYkGDLGYLAQQEQLqkq--------fsNYRYGAFTTREPEnmdenhpsyvgKQYL 241
gi 41614847  118 PLTIEa-----vKQNKKVIVVHQERYkHLLVFRDLFEQL-------------PIIYIPILSKEQSe---------yHGHV 170
gi 160872630 127 TMLAElehrfklRPCFKVVLLFGVRRpEDLLYRDEFIAFsek--------naWFEFHAYYSRVKLnk-------alSYEH 191
gi 58424599  153 SMLPLlae-aiaTRGVQVVLLQGARNpTELLYGDDFRAFada--------hpQFRYVPCFSRDVP-----------EQPH 212
gi 88812917  127 AMLPEler-ridLEGFHVELLLGVRGpEELIYGDEFTAFasq--------cnAFTFRACYSREQPer-------agEFEH 190
gi 52840716  130 AMLNElgqriekHPDLQVVILQGVQRsEEILYPDDFQAFakk--------ypQASFRPYLSRVQK-----------QDLK 190
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1A8P_A       192 TDLMRs--GKLFEDIGlppinpqddrAMICGSPsMLDESCEVLDGFGLkisprm------------------gepgdYLI 251
gi 68550654  221 EELLRk--DVLQNEFNispdp-erthFFVSGQPeMVGHVSEWLSDFGYtrhhp-------------------ddpgeLYI 278
gi 168698110 229 QDLVQs--GELERHLGteldp-atthVFLCGNPrMIGVPVHDRDSGEVrypspvgtveileargftadvaarklkgnVHF 305
gi 32476445  250 QTMFTt--GKLAELVGdplap-gnthVFLCGNPdMIGYVPPGGDVPDRpgmlpllraagf------hdatetpgpgtIRF 320
gi 149179292 242 QDIIQp--ERFQETFGwspap-dkthVFLCGNPsMIGLPEKNDRGELVfpdskgmvellae---qgytlstpknpgnIHF 315
gi 41614847  171 QDYID---RLIYNDAT----------YYICGSRqFVIEIANLLKSKGIn---------------------------kIVI 210
gi 160872630 192 MGYVLnafGEIQPNAKqd-------iVYLCGNPnMVDEVFTLLVKNYGfpt------------------------diIRR 240
gi 58424599  213 ADVRH---GYVQQHLAefvpdaahdiAYLCGNPdMVDICLEALKAAGLps-------------------------aqIRR 264
gi 88812917  191 SGYVQ---GILPNMALnpe----rdiVYLCGNPtMIDEATAFLTENGFsm-------------------------rnVRR 238
gi 52840716  191 DNEYS---GYVQHAFPelnlnptrdmVYLCGNPgMIDEAFNYLKEHGFsm-------------------------qhIVR 242

                 ...
Feature 1          #
1A8P_A       252 ERA 254
gi 68550654  279 EEF 281
gi 168698110 306 EEY 308
gi 32476445  321 EKY 323
gi 149179292 316 EKY 318
gi 41614847  211 EAF 213
gi 160872630 241 EKY 243
gi 58424599  265 EKY 267
gi 88812917  239 EKY 241
gi 52840716  243 EKY 245

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