Conserved Protein Domain Family
SiR

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cd06199: SiR 
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Cytochrome p450- like alpha subunits of E. coli sulfite reductase (SiR) multimerize with beta subunits to catalyze the NADPH dependent reduction of sulfite to sulfide. Beta subunits have an Fe4S4 cluster and a siroheme, while the alpha subunits (cysJ gene) are of the cytochrome p450 (CyPor) family having FAD and FMN as prosthetic groups and utilizing NADPH. Cypor (including cyt -450 reductase, nitric oxide synthase, and methionine synthase reductase) are ferredoxin reductase (FNR)-like proteins with an additional N-terminal FMN domain and a connecting sub-domain inserted within the flavin binding portion of the FNR-like domain. The connecting domain orients the N-terminal FMN domain with the C-terminal FNR domain.
Statistics
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PSSM-Id: 99796
View PSSM: cd06199
Aligned: 31 rows
Threshold Bit Score: 454.378
Threshold Setting Gi: 78486232
Created: 14-May-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 11 residues -Click on image for an interactive view with Cn3D
Feature 1:FAD binding pocket [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                             #                                          
1DDG_A        15 SLSVNQKITGrns--ekdVRHIEIDLGDsGLRYQPGDALGVWYQNDPALVKELVELLWLkgdepv-----------tveg 81
gi 21244055  258 KGPGFRVYAApg----krVRHLEFSLQGsGLSYEPGDALGIRHRNPPALVDAVLQTLRLdgdaav-----------tige 322
gi 46445860   15 TIRNRYQLSKsgs--qknTQHLVLDLRGsQLTYEAGDSIGVYPKHDPELINKTLIAARAsgeeyiq---------skqtg 83
gi 163851659 246 EVIDHVNLNSsrs--dkeTIHLALEFEDgAPAYEPGDSLEIFPENDPQLVDEILRATGLsg------------------- 304
gi 126668341 233 EITELIQLNSsqs--ekaTIHLELGFDGaAPPYKPGDSLDIYPENDAAYVDDLLKAAGLaa------------------- 291
gi 148261784 235 EVAALHPITSsrs--eseTLHLELDLTGsGLSYEPGDTLGIAPTNAPSLADAILGATGLag------------------- 293
gi 183220576 241 TIVTNLVLNDiga--tksTRHIEIKSSL-PVDYLPGDSAGFLAYNREDEVNRILGLLQTdretrv-----------tykg 306
gi 115681586 258 TLLVKEELTNcnvpadkeTIHCELTIEGsKLMYEAGDAVGIYPRNRMEDVEWVIQASGLsrddmcsvps---sayqpqpv 334
gi 159490572 306 TVLALEGLCTlsgpddknTVRMELDLGDsGIGYLPGDALGLYPTNQPQAVEELLRVMGAsasesvpvpswhyeeegvapg 385
gi 66799961  253 KLLVKRVLTKgd----kvGIHLEFELGDsELKYVPGDALAILPDNAASEVSAIISLLKLspsfkvstpgwhyqegeqpnp 328
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1DDG_A        82 ktLPLNEALQWHFELTvn-------taNIVENYAtltrsetll--------------------------------plvgd 122
gi 21244055  323 qtLELHAWLATQRELTkl-------srPFLTAHAeragaqelqallap---------------------tqtaglaslla 374
gi 46445860   84 etISLRDFFSKQGNITdv-------sqKLLKELYnrqtnldkkhqlelll-----------------dennrvalkayla 139
gi 163851659 305 -dEGLRKALLAERDITtlsp---ttveRFAKATGhadaqkfve-------------------------------sgevka 349
gi 126668341 292 -dAALRAELTKSRDVTtl-------slKTLESYAattghqevkalle------------------------sadarawia 339
gi 148261784 294 -dAAAREALIRERDITtl-------tpKLIADYAaltgeaklaala--------------------------adeaarta 339
gi 183220576 307 etWMLYDLLRKKVSIRfl-------pdRVIQKYVslskke-------------------------------------ips 342
gi 115681586 335 snVTLQESLHKYYDLKnv-------rvSLLQSMLrhsvgivkekleillen---------------gtsrsnqalmgyle 392
gi 159490572 386 gsMPLAQALTKCYDIRspkpellrllvAALDGTAndhaanghanghanghaingdaaavppqqrlrdalkdqaaieayle 465
gi 66799961  329 sqITLTHILTKCFDIHnckp----ellQLLKDNVknqqekekltnllaqg-----------------tgksntqlvefle 387
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                              ####               # #            ####    
1DDG_A       123 kakLQHYAAttpivdmvrfspaqLDAEALINLLRPLTPRLYSIASSQae-vENEVHVTVGVVRYDvegraRAGGASSFLA 201
gi 21244055  375 dhqVIDVLRrwp---------adWDHAGLLAALRPLTPRLYSVASSRkr-vGDEVHLTVDEVTYQahghaHLGAASGFLA 444
gi 46445860  140 lheVWDLLLfhv--------evhFTPQELADLLMPLLPRFYSISSSQky-vGEEVHLTIAHLEYEtnghkRRGVCTHYLC 210
gi 163851659 350 wieGRHLIDlier------fpttLTAEHLNTVTRPLPPRAYSIASSRke-vGDEVHLTIAAVRYEthgraRAGVASVHVA 422
gi 126668341 340 grqLIDLLQsfp---------irLSADQLRQITRPLAPRAYSIASSRre-vGEEVHLLISAVRYQsfgraRKGVASGHVA 409
gi 148261784 340 fiaGRQPLDlfea------fphrLDAGQLAGLLRKLPPRYYSIASSQkl-vGDAAHLAIAKVAYEsagrtRLGVASGMVA 412
gi 183220576 343 gklDLDVLLtlyp------sdtkLEIQSIVDILEPIVPRYYSIASSPsahgEEEVHLTVAEVEIEtftgiKTGFCSGFLS 416
gi 115681586 393 taeVGDVLEdfp---------vnLPQTELLANLRPLQPRYYSISSSPvi-dPNKISVTAAIIRCSsrgrsRVGVATTFLQ 462
gi 159490572 466 prhVVDVLAdaa--------pakLTSSQVLSCLRQLQPRLYSISSSPle-aPARVQVTVAEVKYLslgkgRVGVCSTMLS 536
gi 66799961  388 nhhLIDILKmfs--------sarPPIDDLLAQLAKLLPRYYSIASSMse-nKLAVSLCVAVVKYDlhgseRVGIASTHMA 458
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1DDG_A       202 DRve-eEGEVRVFIEHNDNFRLPanpeTPVIMIGPGTGIAPFRAFMQQRaa-------dEAPGKNWLFFGNPHFtEDFLY 273
gi 21244055  445 ALg--eGDTAPVYIEPNERFRVPadadRDILMIGPGTGVAPFRGFVQERae-------tGAKGRNWLFFGAQHFnTDFLY 515
gi 46445860  211 ELvklhNPEVPVFIQPSHSFRLPedlhAPLVMVGPGTGVAPFRAFLQERvl------hhQTKGKHWLFFGERNRsSDFFY 284
gi 163851659 423 DRik-nGAKLRVRVKPNKHFRLPsdpsTDIIMVGPGTGVAPFRAFVQERra-------tEAPGRSWLFFGDRHFtHDFLY 494
gi 126668341 410 DQlk-kGHRLRVKLRPNRHFALPpp-eRDIIMVGPGTGIAPFRAFVQERga-------tGASGRSWLFFGDRQFtHDFLY 480
gi 148261784 413 ERlr-eGDTLPIHIKANPHFRLPedssAPIVMIGAGTGIAPYRAFLQERea-------tGASGRTWLIFGHRRFtHDFLY 484
gi 183220576 417 SLk--eGDTVPFFIQKNNSFRLPsp-dTDIIMIGPGTGIAPFRSFLFEReq-------sGGNGKNWLFFGERNFvSDFYY 486
gi 115681586 463 DRlh-lHDLCPVFISPNPDFRLPqdgtRDIIMIGPGTGIAPFRAFMQERvl-------lGASGKNLLYFGCRHKgKDFIY 534
gi 159490572 537 ERlk-vGARVPVYVHKNPDFRLPadaaQPIIMVGPGTGLAPFRSFILERlleaqasngkKAPGEMVLYFGCRRSdQDYLY 615
gi 66799961  459 DRmn-vGDRVSIFINNNPDFRLPedptTPILMVGPGTGIAPFVSFIQERka-------lGHTGENHLYFGCRRSdEDFLY 530
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1DDG_A       274 QVEWQRYvkeGVLTRIDLAWSRDQkEKVYVQDKLREqGAELWRWINDGAHIYVCGDAnRMAKDVEQALLEVIAEFGGmdt 353
gi 21244055  516 QAEWQLAlrrGELHALDLAFSRDQaEKLYVQHRLRArGAEVYAWLQGGAHVYVCGAT-SMGKDVHAALLDIVATHGAqda 594
gi 46445860  285 EEDWRLFe-qHGHLRLDAVFSRDQeEKVYVQHKMFEhGEELFKWLEEGAYLFVCGDAeRMAKDVEITLLAIIQEFGKkdf 363
gi 163851659 495 QLEWQDAledGSLAKIDVAFSRDQpEKIYVQDRIDAhAAEVVEWLDGGAHFYVCGDAkNMAKDVRAAVVRAFESVKGlss 574
gi 126668341 481 QLEWQDAlkdGSLTRMDVAFSRDTpEKLYVQHLIWAqRRDLVDWLDNGAYFYVCGDAkAMAKDVRAALVRAYADVKAqsl 560
gi 148261784 485 QLEIQDWlksGVLNEIDLAFSRDQpEKRYVQHVLWErRAALQAQLAEGATLYLCGDAkAMARDVDATLAHILGGDDAa-- 562
gi 183220576 487 QTELLELmdtGVLHKLNTAFSRDTkEKVYVQDRMGEnAEELLKWIQNGAVIYLCGSKdPMSKDVDRKLIEILSERTFdtg 566
gi 115681586 535 QKELEQMv-aEGHLKLRTAFSRDGeEKIYVQHLILQdGQMIWNMIQGGAHVYVCGDAkHMAADVHHALLSVIKTEGCmae 613
gi 159490572 616 GDVLEGWa-aEGKIKLFTAFSRQQaQKVYVQNRLTEsADLVWGLLEKGAHFYVCGDAsSMAGSVEKALLQLIGERLEagp 694
gi 66799961  531 SKELQQYh-nDGLIKLYTAFSRETsQKVYVQNRLLEnSQQICDLINAGGHIYICGDAkSMAPQVHETLSLIITKHMSide 609
                        410       420
                 ....*....|....*....|.
Feature 1                             
1DDG_A       354 eAADEFLSELrvERRYQRDVY 374
gi 21244055  595 eAAAAYLTQLqvEGRYARDVY 615
gi 46445860  364 qEAREYVKRLrqQKKYLRDVY 384
gi 163851659 575 aDAEAHVASLerARRYQQDVY 595
gi 126668341 561 eAGEQAVNDLdgEHRYLQDTY 581
gi 148261784 563 -KGQAALDALiaAGRYKKDVY 582
gi 183220576 567 kEASDYLKELeeNGRYIKDVY 587
gi 115681586 614 eCALSYIKELeeTHRYQKDVW 634
gi 159490572 695 aGADAYLQKLsdTGRYQRDVW 715
gi 66799961  610 aDAQALLHKLekEKRYQKDVW 630

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